Experiment: Ying_Others16 rep B; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hyaB and hyaC are separated by 18 nucleotides hyaC and hyaD overlap by 4 nucleotides hyaD and hyaE overlap by 4 nucleotides hyaE and hyaF overlap by 4 nucleotides
b0973: hyaB - hydrogenase 1, large subunit (NCBI), at 1,032,477 to 1,034,270
hyaB
b0974: hyaC - hydrogenase 1, b-type cytochrome subunit (NCBI), at 1,034,289 to 1,034,996
hyaC
b0975: hyaD - protein involved in processing of HyaA and HyaB proteins (NCBI), at 1,034,993 to 1,035,580
hyaD
b0976: hyaE - protein involved in processing of HyaA and HyaB proteins (NCBI), at 1,035,577 to 1,035,975
hyaE
b0977: hyaF - protein involved in nickel incorporation into hydrogenase-1 proteins (NCBI), at 1,035,972 to 1,036,829
hyaF
Position (kb)
1034
1035 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1033.392 kb on - strand, within hyaB at 1033.419 kb on + strand, within hyaB at 1033.419 kb on + strand, within hyaB at 1033.517 kb on - strand, within hyaB at 1033.517 kb on - strand, within hyaB at 1033.529 kb on + strand, within hyaB at 1033.742 kb on + strand, within hyaB at 1033.742 kb on + strand, within hyaB at 1033.875 kb on - strand, within hyaB at 1033.960 kb on + strand, within hyaB at 1033.960 kb on + strand, within hyaB at 1034.059 kb on + strand, within hyaB at 1034.059 kb on + strand, within hyaB at 1034.191 kb on + strand at 1034.236 kb on - strand at 1034.252 kb on - strand at 1034.276 kb on + strand at 1034.276 kb on + strand at 1034.319 kb on + strand at 1034.319 kb on + strand at 1034.408 kb on - strand, within hyaC at 1034.577 kb on - strand, within hyaC at 1034.720 kb on - strand, within hyaC at 1034.720 kb on - strand, within hyaC at 1034.890 kb on - strand, within hyaC at 1034.895 kb on - strand, within hyaC at 1034.940 kb on + strand at 1034.940 kb on + strand at 1034.945 kb on + strand at 1034.947 kb on - strand at 1035.102 kb on + strand, within hyaD at 1035.201 kb on + strand, within hyaD at 1035.201 kb on + strand, within hyaD at 1035.291 kb on - strand, within hyaD at 1035.291 kb on - strand, within hyaD at 1035.310 kb on + strand, within hyaD at 1035.437 kb on + strand, within hyaD at 1035.437 kb on + strand, within hyaD at 1035.495 kb on + strand, within hyaD at 1035.503 kb on - strand, within hyaD at 1035.503 kb on - strand, within hyaD at 1035.803 kb on + strand, within hyaE at 1035.813 kb on + strand, within hyaE at 1035.892 kb on + strand, within hyaE at 1035.910 kb on - strand, within hyaE at 1035.910 kb on - strand, within hyaE at 1035.990 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Others16 rep B; time point 1 remove 1,033,392 - hyaB b0973 0.51 -0.0 1,033,419 + hyaB b0973 0.53 +1.0 1,033,419 + hyaB b0973 0.53 -0.4 1,033,517 - hyaB b0973 0.58 -1.1 1,033,517 - hyaB b0973 0.58 +0.1 1,033,529 + hyaB b0973 0.59 -0.7 1,033,742 + hyaB b0973 0.71 +1.1 1,033,742 + hyaB b0973 0.71 +1.5 1,033,875 - hyaB b0973 0.78 +0.4 1,033,960 + hyaB b0973 0.83 +1.5 1,033,960 + hyaB b0973 0.83 -0.7 1,034,059 + hyaB b0973 0.88 +0.2 1,034,059 + hyaB b0973 0.88 +1.0 1,034,191 + -0.2 1,034,236 - -0.3 1,034,252 - +0.5 1,034,276 + +1.6 1,034,276 + +0.2 1,034,319 + +0.7 1,034,319 + -0.8 1,034,408 - hyaC b0974 0.17 +0.2 1,034,577 - hyaC b0974 0.41 -0.1 1,034,720 - hyaC b0974 0.61 +0.5 1,034,720 - hyaC b0974 0.61 +0.5 1,034,890 - hyaC b0974 0.85 +1.3 1,034,895 - hyaC b0974 0.86 +1.0 1,034,940 + -0.6 1,034,940 + +0.4 1,034,945 + +0.1 1,034,947 - +0.7 1,035,102 + hyaD b0975 0.19 -0.8 1,035,201 + hyaD b0975 0.35 -0.2 1,035,201 + hyaD b0975 0.35 +1.8 1,035,291 - hyaD b0975 0.51 +1.1 1,035,291 - hyaD b0975 0.51 -0.7 1,035,310 + hyaD b0975 0.54 +0.1 1,035,437 + hyaD b0975 0.76 +0.2 1,035,437 + hyaD b0975 0.76 -0.5 1,035,495 + hyaD b0975 0.85 +0.1 1,035,503 - hyaD b0975 0.87 +0.4 1,035,503 - hyaD b0975 0.87 -1.6 1,035,803 + hyaE b0976 0.57 -0.5 1,035,813 + hyaE b0976 0.59 -1.1 1,035,892 + hyaE b0976 0.79 +0.8 1,035,910 - hyaE b0976 0.83 +0.2 1,035,910 - hyaE b0976 0.83 -1.9 1,035,990 + -0.4
Or see this region's nucleotide sequence