Experiment: Ying_Others16 rep B; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt leuC and leuB are separated by 2 nucleotides leuB and leuA overlap by 1 nucleotides
b0072: leuC - isopropylmalate isomerase large subunit (NCBI), at 79,464 to 80,864
leuC
b0073: leuB - 3-isopropylmalate dehydrogenase (VIMSS), at 80,867 to 81,958
leuB
b0074: leuA - 2-isopropylmalate synthase (NCBI), at 81,958 to 83,529
leuA
Position (kb)
80
81
82 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 79.883 kb on + strand, within leuC at 79.888 kb on + strand, within leuC at 79.896 kb on - strand, within leuC at 79.931 kb on + strand, within leuC at 80.049 kb on - strand, within leuC at 80.049 kb on - strand, within leuC at 80.077 kb on + strand, within leuC at 80.077 kb on + strand, within leuC at 80.092 kb on - strand, within leuC at 80.120 kb on - strand, within leuC at 80.120 kb on - strand, within leuC at 80.141 kb on + strand, within leuC at 80.141 kb on + strand, within leuC at 80.165 kb on + strand, within leuC at 80.210 kb on + strand, within leuC at 80.210 kb on + strand, within leuC at 80.218 kb on - strand, within leuC at 80.218 kb on - strand, within leuC at 80.236 kb on - strand, within leuC at 80.349 kb on + strand, within leuC at 80.387 kb on + strand at 80.387 kb on + strand, within leuC at 80.414 kb on - strand, within leuC at 80.438 kb on - strand, within leuC at 80.671 kb on - strand, within leuC at 80.671 kb on - strand, within leuC at 80.671 kb on - strand, within leuC at 80.671 kb on - strand, within leuC at 80.714 kb on - strand, within leuC at 80.714 kb on - strand, within leuC at 80.785 kb on + strand at 80.785 kb on + strand at 80.893 kb on - strand at 80.893 kb on - strand at 80.918 kb on + strand at 80.918 kb on + strand at 80.929 kb on - strand at 80.965 kb on + strand at 80.973 kb on - strand at 80.986 kb on - strand, within leuB at 81.032 kb on - strand, within leuB at 81.037 kb on + strand, within leuB at 81.114 kb on + strand, within leuB at 81.114 kb on + strand, within leuB at 81.122 kb on - strand, within leuB at 81.191 kb on - strand, within leuB at 81.191 kb on - strand, within leuB at 81.224 kb on + strand, within leuB at 81.284 kb on + strand, within leuB at 81.284 kb on + strand, within leuB at 81.287 kb on + strand, within leuB at 81.290 kb on - strand, within leuB at 81.292 kb on - strand, within leuB at 81.292 kb on - strand, within leuB at 81.314 kb on - strand, within leuB at 81.404 kb on + strand, within leuB at 81.409 kb on - strand, within leuB at 81.457 kb on + strand, within leuB at 81.480 kb on + strand, within leuB at 81.554 kb on - strand, within leuB at 81.580 kb on + strand, within leuB at 81.607 kb on - strand, within leuB at 81.692 kb on + strand, within leuB at 81.692 kb on + strand, within leuB at 81.910 kb on + strand at 81.910 kb on + strand at 81.922 kb on - strand at 82.051 kb on + strand at 82.135 kb on + strand, within leuA at 82.243 kb on + strand, within leuA at 82.243 kb on + strand, within leuA at 82.246 kb on - strand, within leuA at 82.425 kb on + strand, within leuA at 82.462 kb on - strand, within leuA at 82.462 kb on - strand, within leuA at 82.502 kb on - strand, within leuA at 82.506 kb on - strand, within leuA at 82.624 kb on + strand, within leuA at 82.632 kb on - strand, within leuA at 82.635 kb on + strand, within leuA at 82.637 kb on + strand, within leuA at 82.638 kb on - strand, within leuA at 82.638 kb on - strand, within leuA at 82.647 kb on + strand, within leuA at 82.671 kb on - strand, within leuA at 82.671 kb on - strand, within leuA at 82.671 kb on - strand, within leuA at 82.713 kb on + strand, within leuA at 82.860 kb on + strand, within leuA at 82.860 kb on + strand, within leuA at 82.870 kb on + strand, within leuA at 82.878 kb on - strand, within leuA
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Others16 rep B; time point 1 remove 79,883 + leuC b0072 0.30 -1.9 79,888 + leuC b0072 0.30 -0.7 79,896 - leuC b0072 0.31 -0.3 79,931 + leuC b0072 0.33 +0.5 80,049 - leuC b0072 0.42 -0.9 80,049 - leuC b0072 0.42 -2.1 80,077 + leuC b0072 0.44 -0.3 80,077 + leuC b0072 0.44 -0.5 80,092 - leuC b0072 0.45 -0.1 80,120 - leuC b0072 0.47 -0.9 80,120 - leuC b0072 0.47 -4.6 80,141 + leuC b0072 0.48 +0.2 80,141 + leuC b0072 0.48 +0.8 80,165 + leuC b0072 0.50 -0.3 80,210 + leuC b0072 0.53 -0.2 80,210 + leuC b0072 0.53 -0.5 80,218 - leuC b0072 0.54 -1.4 80,218 - leuC b0072 0.54 -1.4 80,236 - leuC b0072 0.55 -0.8 80,349 + leuC b0072 0.63 -0.8 80,387 + -2.1 80,387 + leuC b0072 0.66 -0.3 80,414 - leuC b0072 0.68 -0.1 80,438 - leuC b0072 0.70 -0.1 80,671 - leuC b0072 0.86 -0.7 80,671 - leuC b0072 0.86 -0.9 80,671 - leuC b0072 0.86 -0.2 80,671 - leuC b0072 0.86 -0.2 80,714 - leuC b0072 0.89 +0.1 80,714 - leuC b0072 0.89 -0.5 80,785 + +0.4 80,785 + +1.5 80,893 - -0.1 80,893 - -0.5 80,918 + -1.5 80,918 + -0.3 80,929 - -0.7 80,965 + -1.2 80,973 - -1.7 80,986 - leuB b0073 0.11 -0.6 81,032 - leuB b0073 0.15 -0.9 81,037 + leuB b0073 0.16 -0.5 81,114 + leuB b0073 0.23 -0.5 81,114 + leuB b0073 0.23 -0.1 81,122 - leuB b0073 0.23 -1.5 81,191 - leuB b0073 0.30 -0.3 81,191 - leuB b0073 0.30 -0.1 81,224 + leuB b0073 0.33 +0.1 81,284 + leuB b0073 0.38 -2.2 81,284 + leuB b0073 0.38 +0.0 81,287 + leuB b0073 0.38 +0.2 81,290 - leuB b0073 0.39 -1.1 81,292 - leuB b0073 0.39 -1.5 81,292 - leuB b0073 0.39 -0.1 81,314 - leuB b0073 0.41 -1.1 81,404 + leuB b0073 0.49 +0.0 81,409 - leuB b0073 0.50 -0.4 81,457 + leuB b0073 0.54 -1.2 81,480 + leuB b0073 0.56 -1.1 81,554 - leuB b0073 0.63 -2.3 81,580 + leuB b0073 0.65 -1.2 81,607 - leuB b0073 0.68 -2.0 81,692 + leuB b0073 0.76 +0.5 81,692 + leuB b0073 0.76 -0.4 81,910 + -1.1 81,910 + -2.2 81,922 - -0.7 82,051 + -0.1 82,135 + leuA b0074 0.11 +0.2 82,243 + leuA b0074 0.18 +1.4 82,243 + leuA b0074 0.18 -0.5 82,246 - leuA b0074 0.18 -0.6 82,425 + leuA b0074 0.30 -0.6 82,462 - leuA b0074 0.32 -0.3 82,462 - leuA b0074 0.32 +1.2 82,502 - leuA b0074 0.35 -1.0 82,506 - leuA b0074 0.35 -0.3 82,624 + leuA b0074 0.42 -1.5 82,632 - leuA b0074 0.43 -1.2 82,635 + leuA b0074 0.43 -0.3 82,637 + leuA b0074 0.43 +0.3 82,638 - leuA b0074 0.43 -0.7 82,638 - leuA b0074 0.43 -0.4 82,647 + leuA b0074 0.44 -1.1 82,671 - leuA b0074 0.45 -0.8 82,671 - leuA b0074 0.45 -0.5 82,671 - leuA b0074 0.45 -1.4 82,713 + leuA b0074 0.48 -1.3 82,860 + leuA b0074 0.57 -1.4 82,860 + leuA b0074 0.57 -2.1 82,870 + leuA b0074 0.58 -0.3 82,878 - leuA b0074 0.59 +0.6
Or see this region's nucleotide sequence