Experiment: Ying_Others16 rep B; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yaaH and yaaW are separated by 148 nucleotides yaaW and yaaI are separated by 25 nucleotides yaaI and dnaK are separated by 376 nucleotides
b0010: yaaH - conserved inner membrane protein associated with acetate transport (NCBI), at 9,928 to 10,494
yaaH
b0011: yaaW - hypothetical protein (NCBI), at 10,643 to 11,356
yaaW
b0013: yaaI - hypothetical protein (NCBI), at 11,382 to 11,786
yaaI
b0014: dnaK - molecular chaperone DnaK (NCBI), at 12,163 to 14,079
dnaK
Position (kb)
11
12 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 10.414 kb on + strand, within yaaH at 10.425 kb on + strand, within yaaH at 10.445 kb on + strand at 10.445 kb on + strand at 10.455 kb on + strand at 10.464 kb on + strand at 10.487 kb on - strand at 10.489 kb on + strand at 10.489 kb on + strand at 10.594 kb on + strand at 10.646 kb on + strand at 10.648 kb on + strand at 10.656 kb on - strand at 10.664 kb on - strand at 10.731 kb on + strand, within yaaW at 10.731 kb on + strand, within yaaW at 10.761 kb on - strand, within yaaW at 10.762 kb on - strand, within yaaW at 10.763 kb on - strand, within yaaW at 10.766 kb on + strand, within yaaW at 10.766 kb on + strand, within yaaW at 10.795 kb on + strand, within yaaW at 10.808 kb on - strand, within yaaW at 10.936 kb on - strand, within yaaW at 10.936 kb on - strand, within yaaW at 10.963 kb on - strand, within yaaW at 10.963 kb on - strand, within yaaW at 11.196 kb on - strand, within yaaW at 11.196 kb on - strand, within yaaW at 11.235 kb on - strand, within yaaW at 11.235 kb on - strand, within yaaW at 11.304 kb on + strand at 11.398 kb on - strand at 11.398 kb on - strand at 11.399 kb on + strand at 11.399 kb on + strand at 11.410 kb on + strand at 11.416 kb on + strand at 11.422 kb on + strand at 11.432 kb on - strand, within yaaI at 11.506 kb on - strand, within yaaI at 11.506 kb on - strand, within yaaI at 11.541 kb on - strand, within yaaI at 11.546 kb on + strand, within yaaI at 11.546 kb on + strand, within yaaI at 11.601 kb on + strand, within yaaI at 11.613 kb on + strand, within yaaI at 11.614 kb on - strand, within yaaI at 11.651 kb on + strand, within yaaI at 11.718 kb on + strand, within yaaI at 11.718 kb on + strand, within yaaI at 12.025 kb on + strand at 12.057 kb on + strand at 12.141 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Others16 rep B; time point 1 remove 10,414 + yaaH b0010 0.86 +0.3 10,425 + yaaH b0010 0.88 +0.9 10,445 + -0.9 10,445 + -0.8 10,455 + -0.4 10,464 + -0.3 10,487 - +0.6 10,489 + -0.8 10,489 + +0.2 10,594 + +0.3 10,646 + -0.1 10,648 + -1.1 10,656 - -0.2 10,664 - +0.3 10,731 + yaaW b0011 0.12 +0.2 10,731 + yaaW b0011 0.12 -2.2 10,761 - yaaW b0011 0.17 +0.8 10,762 - yaaW b0011 0.17 -0.1 10,763 - yaaW b0011 0.17 +0.3 10,766 + yaaW b0011 0.17 -0.2 10,766 + yaaW b0011 0.17 -0.7 10,795 + yaaW b0011 0.21 -3.0 10,808 - yaaW b0011 0.23 -0.2 10,936 - yaaW b0011 0.41 +0.4 10,936 - yaaW b0011 0.41 +0.4 10,963 - yaaW b0011 0.45 -0.6 10,963 - yaaW b0011 0.45 +0.5 11,196 - yaaW b0011 0.77 -1.5 11,196 - yaaW b0011 0.77 -0.5 11,235 - yaaW b0011 0.83 +0.5 11,235 - yaaW b0011 0.83 -0.4 11,304 + -0.0 11,398 - -0.2 11,398 - -0.2 11,399 + -0.1 11,399 + -0.1 11,410 + -0.6 11,416 + -0.2 11,422 + -1.7 11,432 - yaaI b0013 0.12 +1.0 11,506 - yaaI b0013 0.31 -1.4 11,506 - yaaI b0013 0.31 -0.2 11,541 - yaaI b0013 0.39 -0.5 11,546 + yaaI b0013 0.40 -0.5 11,546 + yaaI b0013 0.40 -0.6 11,601 + yaaI b0013 0.54 -0.7 11,613 + yaaI b0013 0.57 -1.3 11,614 - yaaI b0013 0.57 -0.6 11,651 + yaaI b0013 0.66 -0.9 11,718 + yaaI b0013 0.83 -0.3 11,718 + yaaI b0013 0.83 -2.6 12,025 + -0.5 12,057 + +0.3 12,141 + -0.3
Or see this region's nucleotide sequence