Experiment: Ying_Others16 rep B; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thrL and thrA are separated by 81 nucleotides thrA and thrB are separated by 1 nucleotides thrB and thrC are separated by 0 nucleotides
b0001: thrL - thr operon leader peptide (NCBI), at 190 to 255
thrL
b0002: thrA - bifunctional aspartokinase I/homeserine dehydrogenase I (NCBI), at 337 to 2,799
thrA
b0003: thrB - homoserine kinase (NCBI), at 2,801 to 3,733
thrB
b0004: thrC - threonine synthase (NCBI), at 3,734 to 5,020
thrC
Position (kb)
0
1
2
3 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 0.070 kb on + strand at 0.070 kb on + strand at 0.078 kb on - strand at 0.078 kb on - strand at 0.161 kb on - strand at 0.185 kb on - strand at 0.185 kb on - strand at 0.208 kb on + strand, within thrL at 0.217 kb on - strand, within thrL at 0.257 kb on - strand at 0.257 kb on - strand at 0.440 kb on - strand at 0.440 kb on - strand at 0.454 kb on - strand at 0.518 kb on - strand at 0.518 kb on - strand at 0.528 kb on + strand at 0.592 kb on + strand, within thrA at 0.592 kb on + strand, within thrA at 0.666 kb on + strand, within thrA at 0.666 kb on + strand, within thrA at 0.675 kb on - strand, within thrA at 0.754 kb on - strand, within thrA at 0.754 kb on - strand, within thrA at 0.767 kb on + strand, within thrA at 0.775 kb on - strand, within thrA at 0.957 kb on + strand, within thrA at 0.957 kb on + strand, within thrA at 1.046 kb on + strand, within thrA at 1.136 kb on - strand, within thrA at 1.142 kb on - strand, within thrA at 1.161 kb on + strand, within thrA at 1.161 kb on + strand, within thrA at 1.161 kb on + strand, within thrA at 1.259 kb on - strand, within thrA at 1.259 kb on - strand, within thrA at 1.262 kb on + strand, within thrA at 1.270 kb on - strand, within thrA at 1.270 kb on - strand, within thrA at 1.280 kb on - strand, within thrA at 1.489 kb on + strand, within thrA at 1.599 kb on - strand, within thrA at 1.606 kb on + strand, within thrA at 1.627 kb on + strand, within thrA at 1.633 kb on - strand, within thrA at 1.633 kb on - strand, within thrA at 1.636 kb on + strand, within thrA at 1.636 kb on + strand, within thrA at 1.637 kb on + strand, within thrA at 1.711 kb on - strand, within thrA at 1.721 kb on - strand, within thrA at 1.767 kb on - strand, within thrA at 1.793 kb on - strand, within thrA at 1.868 kb on + strand, within thrA at 1.868 kb on + strand, within thrA at 1.871 kb on + strand, within thrA at 1.876 kb on - strand, within thrA at 1.881 kb on - strand, within thrA at 1.881 kb on - strand, within thrA at 1.929 kb on + strand, within thrA at 1.929 kb on + strand, within thrA at 1.937 kb on - strand, within thrA at 1.937 kb on - strand, within thrA at 2.076 kb on + strand, within thrA at 2.104 kb on + strand, within thrA at 2.104 kb on + strand, within thrA at 2.266 kb on - strand, within thrA at 2.266 kb on - strand, within thrA at 2.292 kb on - strand, within thrA at 2.294 kb on + strand, within thrA at 2.370 kb on - strand, within thrA at 2.370 kb on - strand, within thrA at 2.449 kb on - strand, within thrA at 2.590 kb on - strand at 2.675 kb on + strand at 2.682 kb on + strand at 2.682 kb on + strand at 2.684 kb on + strand at 2.717 kb on + strand at 2.744 kb on - strand at 2.787 kb on - strand at 2.787 kb on - strand at 2.829 kb on + strand at 2.847 kb on + strand at 2.847 kb on + strand at 2.906 kb on - strand, within thrB at 2.934 kb on + strand, within thrB at 2.963 kb on - strand, within thrB at 3.020 kb on - strand, within thrB at 3.023 kb on + strand, within thrB at 3.131 kb on + strand, within thrB at 3.144 kb on - strand, within thrB at 3.186 kb on + strand, within thrB at 3.246 kb on + strand, within thrB at 3.248 kb on + strand, within thrB at 3.256 kb on + strand, within thrB at 3.293 kb on + strand, within thrB at 3.301 kb on - strand, within thrB at 3.318 kb on - strand, within thrB at 3.411 kb on + strand, within thrB at 3.444 kb on - strand, within thrB at 3.444 kb on - strand, within thrB at 3.504 kb on - strand, within thrB at 3.504 kb on - strand, within thrB at 3.509 kb on - strand, within thrB at 3.511 kb on + strand, within thrB at 3.607 kb on + strand, within thrB at 3.634 kb on - strand, within thrB at 3.638 kb on + strand, within thrB at 3.638 kb on + strand, within thrB at 3.650 kb on + strand at 3.769 kb on + strand at 3.769 kb on + strand at 3.792 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Others16 rep B; time point 1 remove 70 + +0.4 70 + -0.6 78 - +0.9 78 - +0.3 161 - +0.7 185 - +0.4 185 - -2.3 208 + thrL b0001 0.27 -0.3 217 - thrL b0001 0.41 -0.5 257 - -2.0 257 - -0.5 440 - -3.2 440 - -0.0 454 - -0.9 518 - +0.3 518 - -2.5 528 + -0.1 592 + thrA b0002 0.10 -1.1 592 + thrA b0002 0.10 -2.9 666 + thrA b0002 0.13 -0.7 666 + thrA b0002 0.13 +0.7 675 - thrA b0002 0.14 -0.6 754 - thrA b0002 0.17 -0.1 754 - thrA b0002 0.17 -1.1 767 + thrA b0002 0.17 -2.4 775 - thrA b0002 0.18 -1.1 957 + thrA b0002 0.25 -2.6 957 + thrA b0002 0.25 -2.4 1,046 + thrA b0002 0.29 +0.1 1,136 - thrA b0002 0.32 -0.7 1,142 - thrA b0002 0.33 -0.6 1,161 + thrA b0002 0.33 -1.2 1,161 + thrA b0002 0.33 -0.4 1,161 + thrA b0002 0.33 -1.4 1,259 - thrA b0002 0.37 -0.9 1,259 - thrA b0002 0.37 -2.7 1,262 + thrA b0002 0.38 -0.3 1,270 - thrA b0002 0.38 -0.6 1,270 - thrA b0002 0.38 -2.1 1,280 - thrA b0002 0.38 -1.5 1,489 + thrA b0002 0.47 -0.1 1,599 - thrA b0002 0.51 -0.4 1,606 + thrA b0002 0.52 -1.4 1,627 + thrA b0002 0.52 -2.3 1,633 - thrA b0002 0.53 -0.3 1,633 - thrA b0002 0.53 -0.3 1,636 + thrA b0002 0.53 +0.3 1,636 + thrA b0002 0.53 +0.5 1,637 + thrA b0002 0.53 -0.4 1,711 - thrA b0002 0.56 -3.1 1,721 - thrA b0002 0.56 -0.2 1,767 - thrA b0002 0.58 -1.3 1,793 - thrA b0002 0.59 -0.9 1,868 + thrA b0002 0.62 -0.5 1,868 + thrA b0002 0.62 +0.5 1,871 + thrA b0002 0.62 -0.6 1,876 - thrA b0002 0.62 +0.3 1,881 - thrA b0002 0.63 -1.4 1,881 - thrA b0002 0.63 -1.9 1,929 + thrA b0002 0.65 -0.5 1,929 + thrA b0002 0.65 -0.2 1,937 - thrA b0002 0.65 -2.1 1,937 - thrA b0002 0.65 -1.3 2,076 + thrA b0002 0.71 -0.4 2,104 + thrA b0002 0.72 +0.3 2,104 + thrA b0002 0.72 -1.5 2,266 - thrA b0002 0.78 -1.0 2,266 - thrA b0002 0.78 -1.7 2,292 - thrA b0002 0.79 -0.1 2,294 + thrA b0002 0.79 -1.2 2,370 - thrA b0002 0.83 -1.6 2,370 - thrA b0002 0.83 +0.2 2,449 - thrA b0002 0.86 -0.5 2,590 - -2.4 2,675 + +0.0 2,682 + +0.7 2,682 + +1.3 2,684 + +0.5 2,717 + +0.0 2,744 - +0.2 2,787 - +0.4 2,787 - +0.1 2,829 + -0.7 2,847 + -3.6 2,847 + -1.2 2,906 - thrB b0003 0.11 +0.8 2,934 + thrB b0003 0.14 -0.9 2,963 - thrB b0003 0.17 -1.0 3,020 - thrB b0003 0.23 +0.8 3,023 + thrB b0003 0.24 -1.6 3,131 + thrB b0003 0.35 -1.8 3,144 - thrB b0003 0.37 -1.6 3,186 + thrB b0003 0.41 -0.3 3,246 + thrB b0003 0.48 -1.4 3,248 + thrB b0003 0.48 -1.3 3,256 + thrB b0003 0.49 -1.1 3,293 + thrB b0003 0.53 -0.4 3,301 - thrB b0003 0.54 +0.4 3,318 - thrB b0003 0.55 -0.7 3,411 + thrB b0003 0.65 -0.7 3,444 - thrB b0003 0.69 +0.1 3,444 - thrB b0003 0.69 -1.4 3,504 - thrB b0003 0.75 -0.3 3,504 - thrB b0003 0.75 +0.5 3,509 - thrB b0003 0.76 -0.6 3,511 + thrB b0003 0.76 -2.2 3,607 + thrB b0003 0.86 -0.2 3,634 - thrB b0003 0.89 +0.4 3,638 + thrB b0003 0.90 -0.5 3,638 + thrB b0003 0.90 +0.1 3,650 + -3.6 3,769 + -0.1 3,769 + -0.9 3,792 - -1.1
Or see this region's nucleotide sequence