Experiment: Ying_Others16 rep A; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yegL and ryeC are separated by 180 nucleotides ryeC and ibsA overlap by 103 nucleotides ibsA and ryeD are separated by 235 nucleotides ryeD and ibsB overlap by 99 nucleotides ibsB and b2074 are separated by 278 nucleotides b2074 and mdtB overlap by 1 nucleotides
b2073: yegL - hypothetical protein (NCBI), at 2,150,493 to 2,151,152
yegL
b4436: ryeC - Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes unknown (RefSeq), at 2,151,333 to 2,151,475
ryeC
b4667: ibsA - toxic membrane protein (RefSeq), at 2,151,373 to 2,151,432
ibsA
b4437: ryeD - Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes (RefSeq), at 2,151,668 to 2,151,803
ryeD
b4668: ibsB - toxic membrane protein (RefSeq), at 2,151,705 to 2,151,761
ibsB
b2074: b2074 - putative membrane protein (VIMSS), at 2,152,040 to 2,153,287
b2074
b2075: mdtB - multidrug efflux system, subunit B (NCBI), at 2,153,287 to 2,156,409
mdtB
Position (kb)
2152
2153
2154 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2151.046 kb on - strand, within yegL at 2151.104 kb on - strand at 2151.104 kb on - strand at 2151.195 kb on - strand at 2151.277 kb on + strand at 2151.299 kb on - strand at 2151.311 kb on - strand at 2151.339 kb on + strand at 2151.339 kb on - strand at 2151.354 kb on + strand, within ryeC at 2151.354 kb on + strand, within ryeC at 2151.388 kb on - strand at 2151.411 kb on + strand at 2151.411 kb on + strand at 2151.432 kb on - strand at 2151.512 kb on - strand at 2151.671 kb on + strand at 2151.747 kb on + strand at 2151.747 kb on + strand at 2152.197 kb on + strand, within b2074 at 2152.228 kb on + strand, within b2074 at 2152.253 kb on + strand, within b2074 at 2152.447 kb on + strand, within b2074 at 2152.677 kb on - strand, within b2074 at 2152.677 kb on - strand, within b2074 at 2152.748 kb on + strand, within b2074 at 2152.748 kb on + strand, within b2074 at 2153.105 kb on + strand, within b2074 at 2153.105 kb on + strand, within b2074 at 2153.113 kb on + strand, within b2074 at 2153.113 kb on - strand, within b2074 at 2153.113 kb on - strand, within b2074 at 2153.115 kb on + strand, within b2074 at 2153.343 kb on - strand at 2153.359 kb on - strand at 2153.359 kb on - strand at 2153.415 kb on - strand at 2153.471 kb on + strand at 2153.581 kb on + strand at 2153.581 kb on + strand at 2153.695 kb on + strand, within mdtB at 2153.695 kb on + strand, within mdtB at 2153.753 kb on + strand, within mdtB at 2153.753 kb on + strand, within mdtB at 2153.984 kb on + strand, within mdtB at 2154.025 kb on + strand, within mdtB at 2154.086 kb on + strand, within mdtB at 2154.103 kb on - strand, within mdtB at 2154.231 kb on + strand, within mdtB at 2154.240 kb on - strand, within mdtB at 2154.260 kb on + strand, within mdtB at 2154.260 kb on + strand, within mdtB
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Others16 rep A; time point 1 remove 2,151,046 - yegL b2073 0.84 -0.5 2,151,104 - +0.1 2,151,104 - -0.5 2,151,195 - +1.6 2,151,277 + -0.1 2,151,299 - -1.5 2,151,311 - +0.5 2,151,339 + +0.0 2,151,339 - +0.0 2,151,354 + ryeC b4436 0.15 -0.0 2,151,354 + ryeC b4436 0.15 +0.6 2,151,388 - +0.5 2,151,411 + +0.1 2,151,411 + -0.1 2,151,432 - +0.4 2,151,512 - +0.1 2,151,671 + -0.3 2,151,747 + -0.4 2,151,747 + +0.3 2,152,197 + b2074 b2074 0.13 -0.3 2,152,228 + b2074 b2074 0.15 +0.1 2,152,253 + b2074 b2074 0.17 +0.7 2,152,447 + b2074 b2074 0.33 +0.2 2,152,677 - b2074 b2074 0.51 +0.7 2,152,677 - b2074 b2074 0.51 +0.1 2,152,748 + b2074 b2074 0.57 +1.2 2,152,748 + b2074 b2074 0.57 +0.3 2,153,105 + b2074 b2074 0.85 -0.0 2,153,105 + b2074 b2074 0.85 +1.0 2,153,113 + b2074 b2074 0.86 +1.3 2,153,113 - b2074 b2074 0.86 +0.5 2,153,113 - b2074 b2074 0.86 +0.3 2,153,115 + b2074 b2074 0.86 +0.4 2,153,343 - +0.5 2,153,359 - +0.3 2,153,359 - -3.2 2,153,415 - +0.4 2,153,471 + +1.1 2,153,581 + +0.5 2,153,581 + -0.3 2,153,695 + mdtB b2075 0.13 -0.5 2,153,695 + mdtB b2075 0.13 -1.3 2,153,753 + mdtB b2075 0.15 +0.4 2,153,753 + mdtB b2075 0.15 -0.7 2,153,984 + mdtB b2075 0.22 -0.9 2,154,025 + mdtB b2075 0.24 +0.4 2,154,086 + mdtB b2075 0.26 +0.2 2,154,103 - mdtB b2075 0.26 -0.8 2,154,231 + mdtB b2075 0.30 -0.8 2,154,240 - mdtB b2075 0.31 -1.6 2,154,260 + mdtB b2075 0.31 +0.8 2,154,260 + mdtB b2075 0.31 +1.4
Or see this region's nucleotide sequence