Experiment: Ying_Others16 rep A; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt znuC and znuB overlap by 4 nucleotides znuB and ruvB are separated by 146 nucleotides ruvB and ruvA are separated by 8 nucleotides ruvA and yobI are separated by 138 nucleotides yobI and yebB are separated by 70 nucleotides
b1858: znuC - high-affinity zinc transporter ATPase (NCBI), at 1,940,686 to 1,941,441
znuC
b1859: znuB - high-affinity zinc transporter membrane component (NCBI), at 1,941,438 to 1,942,223
znuB
b1860: ruvB - Holliday junction DNA helicase B (NCBI), at 1,942,370 to 1,943,380
ruvB
b1861: ruvA - Holliday junction DNA helicase motor protein (NCBI), at 1,943,389 to 1,944,000
ruvA
b4677: yobI - expressed protein (RefSeq), at 1,944,139 to 1,944,204
yobI
b1862: yebB - orf, hypothetical protein (VIMSS), at 1,944,275 to 1,944,877
yebB
Position (kb)
1942
1943
1944 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1941.385 kb on - strand at 1941.492 kb on - strand at 1941.587 kb on + strand, within znuB at 1941.587 kb on + strand, within znuB at 1941.589 kb on + strand, within znuB at 1941.662 kb on + strand, within znuB at 1941.662 kb on + strand at 1941.704 kb on + strand, within znuB at 1941.712 kb on - strand at 1941.714 kb on + strand, within znuB at 1941.749 kb on + strand, within znuB at 1941.914 kb on + strand, within znuB at 1941.914 kb on + strand, within znuB at 1941.978 kb on + strand, within znuB at 1942.038 kb on + strand, within znuB at 1942.038 kb on + strand, within znuB at 1942.094 kb on + strand, within znuB at 1942.094 kb on + strand, within znuB at 1942.156 kb on - strand at 1942.156 kb on - strand at 1942.184 kb on + strand at 1942.295 kb on + strand at 1942.295 kb on + strand at 1942.423 kb on + strand at 1942.445 kb on - strand at 1942.446 kb on - strand at 1942.806 kb on + strand, within ruvB at 1942.985 kb on + strand, within ruvB at 1943.892 kb on + strand, within ruvA at 1943.919 kb on - strand, within ruvA at 1943.934 kb on - strand, within ruvA at 1943.990 kb on - strand at 1943.996 kb on + strand at 1943.996 kb on + strand at 1944.021 kb on + strand at 1944.102 kb on + strand at 1944.102 kb on + strand at 1944.137 kb on + strand at 1944.333 kb on - strand at 1944.333 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Others16 rep A; time point 1 remove 1,941,385 - +0.0 1,941,492 - -1.2 1,941,587 + znuB b1859 0.19 -0.8 1,941,587 + znuB b1859 0.19 -0.2 1,941,589 + znuB b1859 0.19 +0.1 1,941,662 + znuB b1859 0.28 -2.6 1,941,662 + +0.9 1,941,704 + znuB b1859 0.34 -2.5 1,941,712 - -1.4 1,941,714 + znuB b1859 0.35 -0.7 1,941,749 + znuB b1859 0.40 -0.5 1,941,914 + znuB b1859 0.61 -2.3 1,941,914 + znuB b1859 0.61 -0.0 1,941,978 + znuB b1859 0.69 +0.0 1,942,038 + znuB b1859 0.76 -3.9 1,942,038 + znuB b1859 0.76 -0.1 1,942,094 + znuB b1859 0.83 -0.7 1,942,094 + znuB b1859 0.83 -0.6 1,942,156 - +1.0 1,942,156 - +0.7 1,942,184 + -1.5 1,942,295 + +0.8 1,942,295 + +1.7 1,942,423 + -1.7 1,942,445 - +0.3 1,942,446 - -2.0 1,942,806 + ruvB b1860 0.43 -1.6 1,942,985 + ruvB b1860 0.61 +0.1 1,943,892 + ruvA b1861 0.82 -1.9 1,943,919 - ruvA b1861 0.87 -1.2 1,943,934 - ruvA b1861 0.89 +0.1 1,943,990 - -1.4 1,943,996 + +2.0 1,943,996 + +0.3 1,944,021 + +0.0 1,944,102 + -1.4 1,944,102 + +1.3 1,944,137 + -0.7 1,944,333 - +0.3 1,944,333 - +0.1
Or see this region's nucleotide sequence