Strain Fitness in Escherichia coli BW25113 around b1614

Experiment: Ying_Others16 rep A; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmanA and ydgA are separated by 100 nucleotidesydgA and uidC are separated by 225 nucleotides b1613: manA - mannose-6-phosphate isomerase (VIMSS), at 1,686,600 to 1,687,775 manA b1614: ydgA - hypothetical protein (NCBI), at 1,687,876 to 1,689,384 ydgA b1615: uidC - membrane-associated protein (VIMSS), at 1,689,610 to 1,690,875 uidC Position (kb) 1687 1688 1689 1690Strain fitness (log2 ratio) -1 0 1 2at 1686.883 kb on - strand, within manAat 1687.299 kb on + strand, within manAat 1687.351 kb on + strand, within manAat 1687.526 kb on - strand, within manAat 1687.932 kb on - strandat 1688.065 kb on + strand, within ydgAat 1688.226 kb on + strand, within ydgAat 1688.573 kb on - strand, within ydgAat 1688.718 kb on - strand, within ydgAat 1688.786 kb on - strand, within ydgAat 1688.901 kb on + strand, within ydgAat 1688.940 kb on + strand, within ydgAat 1689.240 kb on + strandat 1689.593 kb on - strandat 1689.678 kb on - strandat 1689.678 kb on - strandat 1689.759 kb on + strand, within uidCat 1689.777 kb on - strand, within uidCat 1689.777 kb on - strand, within uidCat 1690.093 kb on - strand, within uidCat 1690.093 kb on - strand, within uidCat 1690.166 kb on + strand, within uidCat 1690.208 kb on + strand, within uidCat 1690.209 kb on + strand, within uidCat 1690.291 kb on + strand, within uidCat 1690.308 kb on + strand, within uidCat 1690.368 kb on + strand, within uidC

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Others16 rep A; time point 1
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1,686,883 - manA b1613 0.24 -0.8
1,687,299 + manA b1613 0.59 -0.4
1,687,351 + manA b1613 0.64 +0.2
1,687,526 - manA b1613 0.79 -0.5
1,687,932 - -0.6
1,688,065 + ydgA b1614 0.13 -0.3
1,688,226 + ydgA b1614 0.23 +1.1
1,688,573 - ydgA b1614 0.46 -0.5
1,688,718 - ydgA b1614 0.56 +1.4
1,688,786 - ydgA b1614 0.60 -0.7
1,688,901 + ydgA b1614 0.68 +0.7
1,688,940 + ydgA b1614 0.71 +1.0
1,689,240 + +0.4
1,689,593 - -1.5
1,689,678 - +0.1
1,689,678 - +0.5
1,689,759 + uidC b1615 0.12 -0.4
1,689,777 - uidC b1615 0.13 +0.2
1,689,777 - uidC b1615 0.13 +0.5
1,690,093 - uidC b1615 0.38 +1.9
1,690,093 - uidC b1615 0.38 +0.8
1,690,166 + uidC b1615 0.44 -0.7
1,690,208 + uidC b1615 0.47 -0.4
1,690,209 + uidC b1615 0.47 -0.2
1,690,291 + uidC b1615 0.54 +2.2
1,690,308 + uidC b1615 0.55 +2.2
1,690,368 + uidC b1615 0.60 +2.2

Or see this region's nucleotide sequence