Strain Fitness in Escherichia coli BW25113 around b0943

Experiment: Ying_Others16 rep A; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycbT and ycbU are separated by 11 nucleotidesycbU and b0943 are separated by 7 nucleotidesb0943 and ycbF overlap by 8 nucleotidesycbF and pyrD are separated by 110 nucleotides b0941: ycbT - predicted fimbrial-like adhesin protein (NCBI), at 1,001,030 to 1,002,100 ycbT b0942: ycbU - predicted fimbrial-like adhesin protein (NCBI), at 1,002,112 to 1,002,654 ycbU b0943: b0943 - putative fimbrial-like protein (VIMSS), at 1,002,662 to 1,003,177 b0943 b0944: ycbF - putative chaperone (VIMSS), at 1,003,170 to 1,003,880 ycbF b0945: pyrD - dihydroorotate dehydrogenase (NCBI), at 1,003,991 to 1,005,001 pyrD Position (kb) 1002 1003 1004Strain fitness (log2 ratio) -3 -2 -1 0 1at 1001.765 kb on - strand, within ycbTat 1001.765 kb on - strand, within ycbTat 1001.812 kb on - strand, within ycbTat 1001.915 kb on + strand, within ycbTat 1001.918 kb on - strand, within ycbTat 1002.028 kb on + strandat 1002.036 kb on - strandat 1002.041 kb on - strandat 1002.224 kb on - strand, within ycbUat 1002.323 kb on - strand, within ycbUat 1002.325 kb on + strand, within ycbUat 1002.325 kb on + strand, within ycbUat 1002.418 kb on + strand, within ycbUat 1002.421 kb on - strand, within ycbUat 1002.421 kb on - strand, within ycbUat 1002.522 kb on + strand, within ycbUat 1002.680 kb on - strandat 1002.688 kb on + strandat 1002.688 kb on + strandat 1002.766 kb on + strand, within b0943at 1002.795 kb on - strand, within b0943at 1002.803 kb on + strand, within b0943at 1002.907 kb on - strand, within b0943at 1002.921 kb on + strand, within b0943at 1002.952 kb on - strand, within b0943at 1002.952 kb on - strand, within b0943at 1003.071 kb on - strand, within b0943at 1003.071 kb on - strand, within b0943at 1003.072 kb on - strand, within b0943at 1003.109 kb on + strand, within b0943at 1003.112 kb on + strand, within b0943at 1003.338 kb on + strand, within ycbFat 1003.385 kb on + strand, within ycbFat 1003.393 kb on - strand, within ycbFat 1003.460 kb on - strand, within ycbFat 1003.616 kb on + strand, within ycbFat 1003.627 kb on + strand, within ycbFat 1003.688 kb on - strand, within ycbFat 1003.705 kb on - strand, within ycbFat 1003.705 kb on - strand, within ycbFat 1003.723 kb on + strand, within ycbFat 1003.723 kb on + strand, within ycbFat 1003.775 kb on + strand, within ycbFat 1003.775 kb on + strand, within ycbFat 1003.959 kb on - strandat 1004.039 kb on - strandat 1004.050 kb on - strandat 1004.119 kb on + strand, within pyrDat 1004.126 kb on + strand, within pyrDat 1004.126 kb on + strand, within pyrDat 1004.126 kb on + strand, within pyrDat 1004.134 kb on + strand, within pyrDat 1004.141 kb on + strand, within pyrDat 1004.174 kb on - strand, within pyrD

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Others16 rep A; time point 1
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1,001,765 - ycbT b0941 0.69 +0.6
1,001,765 - ycbT b0941 0.69 +1.4
1,001,812 - ycbT b0941 0.73 -0.3
1,001,915 + ycbT b0941 0.83 -0.6
1,001,918 - ycbT b0941 0.83 -0.3
1,002,028 + +0.4
1,002,036 - +0.2
1,002,041 - -0.3
1,002,224 - ycbU b0942 0.21 +0.7
1,002,323 - ycbU b0942 0.39 -0.4
1,002,325 + ycbU b0942 0.39 +0.1
1,002,325 + ycbU b0942 0.39 +0.2
1,002,418 + ycbU b0942 0.56 +0.1
1,002,421 - ycbU b0942 0.57 -0.6
1,002,421 - ycbU b0942 0.57 -2.9
1,002,522 + ycbU b0942 0.76 -0.1
1,002,680 - -1.1
1,002,688 + +0.2
1,002,688 + +0.3
1,002,766 + b0943 b0943 0.20 +0.5
1,002,795 - b0943 b0943 0.26 +1.1
1,002,803 + b0943 b0943 0.27 +0.6
1,002,907 - b0943 b0943 0.47 +1.2
1,002,921 + b0943 b0943 0.50 -0.4
1,002,952 - b0943 b0943 0.56 +1.5
1,002,952 - b0943 b0943 0.56 +0.4
1,003,071 - b0943 b0943 0.79 +0.6
1,003,071 - b0943 b0943 0.79 +1.3
1,003,072 - b0943 b0943 0.79 -2.8
1,003,109 + b0943 b0943 0.87 -0.3
1,003,112 + b0943 b0943 0.87 -0.1
1,003,338 + ycbF b0944 0.24 -0.1
1,003,385 + ycbF b0944 0.30 -0.9
1,003,393 - ycbF b0944 0.31 -1.4
1,003,460 - ycbF b0944 0.41 +0.8
1,003,616 + ycbF b0944 0.63 -1.5
1,003,627 + ycbF b0944 0.64 -0.9
1,003,688 - ycbF b0944 0.73 -0.8
1,003,705 - ycbF b0944 0.75 +0.5
1,003,705 - ycbF b0944 0.75 +0.9
1,003,723 + ycbF b0944 0.78 -1.1
1,003,723 + ycbF b0944 0.78 +0.0
1,003,775 + ycbF b0944 0.85 -0.6
1,003,775 + ycbF b0944 0.85 +0.3
1,003,959 - +0.7
1,004,039 - -0.3
1,004,050 - -0.4
1,004,119 + pyrD b0945 0.13 -2.4
1,004,126 + pyrD b0945 0.13 -0.9
1,004,126 + pyrD b0945 0.13 -2.0
1,004,126 + pyrD b0945 0.13 -1.1
1,004,134 + pyrD b0945 0.14 +1.2
1,004,141 + pyrD b0945 0.15 -0.5
1,004,174 - pyrD b0945 0.18 -0.9

Or see this region's nucleotide sequence