Strain Fitness in Escherichia coli BW25113 around b3021

Experiment: Ying_Nucleobase14 rep C; time point 1

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Ying_Nucleobase14 rep C; time point 1
3,164,942	+	ygiS	b3020	0.50	+0.1
3,164,953	-	ygiS	b3020	0.51	+0.4
3,164,953	-	ygiS	b3020	0.51	-0.2
3,164,984	-	ygiS	b3020	0.53	-0.8
3,164,984	-	ygiS	b3020	0.53	+0.3
3,164,984	-	ygiS	b3020	0.53	-0.6
3,165,101	+	ygiS	b3020	0.60	-1.3
3,165,112	+	ygiS	b3020	0.61	-0.1
3,165,112	+	ygiS	b3020	0.61	-1.1
3,165,114	+	ygiS	b3020	0.61	-0.1
3,165,122	-	ygiS	b3020	0.62	-0.3
3,165,147	-	ygiS	b3020	0.63	+1.2
3,165,147	-	ygiS	b3020	0.63	-0.0
3,165,196	-	ygiS	b3020	0.66	-0.5
3,165,256	-	ygiS	b3020	0.70	-0.2
3,165,256	-	ygiS	b3020	0.70	+0.3
3,165,304	+	ygiS	b3020	0.73	-0.3
3,165,442	+	ygiS	b3020	0.81	+0.3
3,165,443	-	ygiS	b3020	0.81	-0.5
3,165,477	+	ygiS	b3020	0.84	-0.4
3,165,516	-	ygiS	b3020	0.86	+0.2
3,165,530	-	ygiS	b3020	0.87	+0.0
3,165,530	-	ygiS	b3020	0.87	-0.6
3,165,544	+	ygiS	b3020	0.88	-0.1
3,165,545	-	ygiS	b3020	0.88	+0.0
3,165,545	-	ygiS	b3020	0.88	+0.1
3,165,552	-	ygiS	b3020	0.88	+0.1
3,165,574	+	ygiS	b3020	0.90	+0.6
3,165,583	-				-0.5
3,165,583	-				-1.4
3,165,616	-				-0.3
3,165,667	-				+0.2
3,165,682	+				+0.1
3,165,682	+				-0.4
3,165,687	-				+0.4
3,165,692	+				+0.5
3,165,692	-				+0.0
3,165,692	-				+0.5
3,165,700	+				+0.5
3,165,736	+				-0.0
3,165,745	-				+0.8
3,165,748	-				-0.2
3,165,770	+				-0.2
3,165,775	+				+0.0
3,165,784	-				-1.5
3,165,798	+				-1.5
3,165,798	+				-0.4
3,165,822	+				+0.8
3,165,822	+				+1.4
3,165,895	+				-1.5
3,165,903	+				+0.5
3,165,914	-	ygiT	b3021	0.10	-2.0
3,165,946	-	ygiT	b3021	0.18	+0.2
3,165,950	-	ygiT	b3021	0.19	+0.1
3,165,950	-	ygiT	b3021	0.19	-2.5
3,165,985	-	ygiT	b3021	0.28	-1.7
3,166,018	-	ygiT	b3021	0.37	-2.3
3,166,093	+	ygiT	b3021	0.56	+2.0
3,166,285	-				-0.3
3,166,285	-				-0.4
3,166,286	-				+0.3
3,166,290	-				-0.6
3,166,290	-				-1.3
3,166,299	-				+0.7
3,166,308	-	b3022	b3022	0.13	-0.8
3,166,308	-	b3022	b3022	0.13	+0.4
3,166,325	-	b3022	b3022	0.19	-0.0
3,166,330	-	b3022	b3022	0.20	+0.9
3,166,330	-	b3022	b3022	0.20	-0.1
3,166,335	-	b3022	b3022	0.22	+0.2
3,166,337	+	b3022	b3022	0.23	+1.0
3,166,345	-	b3022	b3022	0.25	-0.3
3,166,345	-	b3022	b3022	0.25	+0.0
3,166,346	-	b3022	b3022	0.26	-0.3
3,166,357	-	b3022	b3022	0.29	-0.7
3,166,370	+	b3022	b3022	0.34	+0.4
3,166,376	-	b3022	b3022	0.36	-0.3
3,166,377	+	b3022	b3022	0.36	-0.8
3,166,382	+	b3022	b3022	0.38	-0.6
3,166,382	+	b3022	b3022	0.38	+1.2
3,166,388	-	b3022	b3022	0.40	+0.0
3,166,388	-	b3022	b3022	0.40	-0.0
3,166,390	-	b3022	b3022	0.40	-0.2
3,166,420	-	b3022	b3022	0.51	-0.5
3,166,441	-	b3022	b3022	0.58	+0.1
3,166,480	-	b3022	b3022	0.71	-0.2
3,166,480	-	b3022	b3022	0.71	+0.7
3,166,523	+	b3022	b3022	0.85	+0.1
3,166,539	+				+0.5
3,166,551	-				-0.6
3,166,551	-				-0.2
3,166,551	-				+0.1
3,166,576	-				-0.2
3,166,576	-				+0.0
3,166,696	-				+0.2
3,166,703	+				+0.0
3,166,703	+				+0.6
3,166,771	-				+0.2
3,166,795	-				+0.7
3,166,795	-				-1.6
3,166,843	+	ygiV	b3023	0.15	+0.2
3,166,909	-	ygiV	b3023	0.29	-0.4
3,166,956	-	ygiV	b3023	0.38	-0.1
3,166,956	-	ygiV	b3023	0.38	-0.2
3,166,989	+	ygiV	b3023	0.45	+0.3
3,166,989	+	ygiV	b3023	0.45	-0.1
3,167,060	+	ygiV	b3023	0.60	-0.5
3,167,154	+	ygiV	b3023	0.79	-0.5
3,167,158	+	ygiV	b3023	0.80	-0.3
3,167,175	+	ygiV	b3023	0.84	+1.1
3,167,175	+	ygiV	b3023	0.84	+0.3
3,167,175	+	ygiV	b3023	0.84	+0.0
3,167,192	+	ygiV	b3023	0.87	-0.1
3,167,227	+				-0.2