Experiment: Ying_Nucleobase14 rep C; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gshA and yqaA are separated by 72 nucleotides yqaA and yqaB overlap by 4 nucleotides yqaB and argQ are separated by 280 nucleotides
b2688: gshA - glutamate--cysteine ligase (NCBI), at 2,812,905 to 2,814,461
gshA
b2689: yqaA - conserved inner membrane protein (NCBI), at 2,814,534 to 2,814,962
yqaA
b2690: yqaB - predicted hydrolase (NCBI), at 2,814,959 to 2,815,525
yqaB
b2691: argQ - tRNA-Arg (NCBI), at 2,815,806 to 2,815,882
argQ
Position (kb)
2814
2815 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2813.616 kb on + strand, within gshA at 2813.624 kb on - strand, within gshA at 2813.779 kb on + strand, within gshA at 2813.812 kb on - strand, within gshA at 2813.878 kb on - strand, within gshA at 2813.881 kb on - strand, within gshA at 2813.915 kb on - strand, within gshA at 2813.915 kb on - strand, within gshA at 2814.108 kb on - strand, within gshA at 2814.115 kb on + strand, within gshA at 2814.131 kb on - strand, within gshA at 2814.131 kb on - strand, within gshA at 2814.139 kb on + strand, within gshA at 2814.139 kb on + strand, within gshA at 2814.144 kb on + strand, within gshA at 2814.191 kb on + strand, within gshA at 2814.233 kb on + strand, within gshA at 2814.233 kb on + strand, within gshA at 2814.303 kb on + strand, within gshA at 2814.308 kb on + strand at 2814.308 kb on + strand at 2814.316 kb on - strand at 2814.331 kb on - strand at 2814.373 kb on + strand at 2814.552 kb on + strand at 2814.552 kb on + strand at 2814.709 kb on - strand, within yqaA at 2814.709 kb on - strand, within yqaA at 2814.745 kb on - strand, within yqaA at 2814.798 kb on - strand, within yqaA at 2814.900 kb on + strand, within yqaA at 2814.900 kb on + strand, within yqaA at 2814.981 kb on - strand at 2814.981 kb on - strand at 2814.983 kb on + strand at 2814.983 kb on + strand at 2814.991 kb on + strand at 2815.035 kb on + strand, within yqaB at 2815.035 kb on + strand, within yqaB at 2815.051 kb on + strand, within yqaB at 2815.059 kb on + strand, within yqaB at 2815.152 kb on + strand, within yqaB at 2815.153 kb on + strand, within yqaB at 2815.194 kb on - strand, within yqaB at 2815.279 kb on + strand, within yqaB at 2815.381 kb on - strand, within yqaB at 2815.430 kb on + strand, within yqaB at 2815.430 kb on + strand, within yqaB at 2815.430 kb on + strand, within yqaB at 2815.460 kb on + strand, within yqaB at 2815.472 kb on + strand at 2815.478 kb on - strand at 2815.480 kb on - strand at 2815.514 kb on - strand at 2815.529 kb on - strand at 2815.922 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 1 remove 2,813,616 + gshA b2688 0.46 +1.5 2,813,624 - gshA b2688 0.46 +1.9 2,813,779 + gshA b2688 0.56 +1.2 2,813,812 - gshA b2688 0.58 +1.8 2,813,878 - gshA b2688 0.62 +1.5 2,813,881 - gshA b2688 0.63 +1.7 2,813,915 - gshA b2688 0.65 +2.3 2,813,915 - gshA b2688 0.65 +2.2 2,814,108 - gshA b2688 0.77 +1.9 2,814,115 + gshA b2688 0.78 +2.3 2,814,131 - gshA b2688 0.79 +3.0 2,814,131 - gshA b2688 0.79 +2.3 2,814,139 + gshA b2688 0.79 +2.1 2,814,139 + gshA b2688 0.79 +1.8 2,814,144 + gshA b2688 0.80 +1.6 2,814,191 + gshA b2688 0.83 +1.2 2,814,233 + gshA b2688 0.85 +0.8 2,814,233 + gshA b2688 0.85 +2.7 2,814,303 + gshA b2688 0.90 +2.3 2,814,308 + +1.5 2,814,308 + +2.8 2,814,316 - +2.3 2,814,331 - +1.9 2,814,373 + +1.9 2,814,552 + +0.7 2,814,552 + +0.7 2,814,709 - yqaA b2689 0.41 -2.7 2,814,709 - yqaA b2689 0.41 -2.6 2,814,745 - yqaA b2689 0.49 -1.9 2,814,798 - yqaA b2689 0.62 -0.7 2,814,900 + yqaA b2689 0.85 -0.1 2,814,900 + yqaA b2689 0.85 -0.5 2,814,981 - -0.4 2,814,981 - -3.4 2,814,983 + -0.0 2,814,983 + -1.2 2,814,991 + -0.3 2,815,035 + yqaB b2690 0.13 -0.9 2,815,035 + yqaB b2690 0.13 +0.1 2,815,051 + yqaB b2690 0.16 -0.1 2,815,059 + yqaB b2690 0.18 +0.5 2,815,152 + yqaB b2690 0.34 -0.0 2,815,153 + yqaB b2690 0.34 -0.5 2,815,194 - yqaB b2690 0.41 -1.6 2,815,279 + yqaB b2690 0.56 +0.8 2,815,381 - yqaB b2690 0.74 -1.2 2,815,430 + yqaB b2690 0.83 -0.0 2,815,430 + yqaB b2690 0.83 +0.1 2,815,430 + yqaB b2690 0.83 -0.3 2,815,460 + yqaB b2690 0.88 -0.2 2,815,472 + -0.1 2,815,478 - -4.0 2,815,480 - -1.8 2,815,514 - -1.7 2,815,529 - +1.4 2,815,922 + -0.4
Or see this region's nucleotide sequence