Strain Fitness in Escherichia coli BW25113 around b2277

Experiment: Ying_Nucleobase14 rep C; time point 1

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Ying_Nucleobase14 rep C; time point 1
2,388,545	+	nuoN	b2276	0.33	-0.6
2,388,665	-	nuoN	b2276	0.41	-0.9
2,388,762	-	nuoN	b2276	0.47	-0.5
2,388,773	-	nuoN	b2276	0.48	-0.1
2,388,835	+	nuoN	b2276	0.52	-0.5
2,388,999	-	nuoN	b2276	0.64	-1.6
2,389,002	+	nuoN	b2276	0.64	-0.7
2,389,005	-	nuoN	b2276	0.64	-1.5
2,389,005	-	nuoN	b2276	0.64	-0.4
2,389,007	+	nuoN	b2276	0.64	-0.3
2,389,258	-	nuoN	b2276	0.81	-0.8
2,389,295	-	nuoN	b2276	0.84	-1.4
2,389,306	-	nuoN	b2276	0.85	-0.8
2,389,306	-	nuoN	b2276	0.85	+1.0
2,389,442	+				-0.8
2,389,461	-				-1.9
2,389,517	+				-0.4
2,389,539	+				-1.5
2,389,787	-	nuoM	b2277	0.17	+0.4
2,389,817	-	nuoM	b2277	0.18	-1.2
2,389,843	-	nuoM	b2277	0.20	-0.2
2,389,938	-	nuoM	b2277	0.26	-1.7
2,389,972	-	nuoM	b2277	0.29	-0.7
2,390,000	-	nuoM	b2277	0.30	-1.9
2,390,028	-	nuoM	b2277	0.32	-1.6
2,390,028	-	nuoM	b2277	0.32	-0.8
2,390,151	-	nuoM	b2277	0.40	-1.1
2,390,151	-	nuoM	b2277	0.40	-1.0
2,390,162	+	nuoM	b2277	0.41	-2.0
2,390,162	+	nuoM	b2277	0.41	-0.7
2,390,162	+	nuoM	b2277	0.41	-1.1
2,390,170	-	nuoM	b2277	0.42	+0.5
2,390,211	+	nuoM	b2277	0.44	+0.7
2,390,230	-	nuoM	b2277	0.45	-0.8
2,390,312	+	nuoM	b2277	0.51	+0.0
2,390,341	+	nuoM	b2277	0.53	-1.1
2,390,383	-	nuoM	b2277	0.55	-1.1
2,390,411	+	nuoM	b2277	0.57	-2.2
2,390,426	-	nuoM	b2277	0.58	-2.2
2,390,454	-	nuoM	b2277	0.60	-1.9
2,390,511	-	nuoM	b2277	0.64	-1.6
2,390,516	-	nuoM	b2277	0.64	-1.6
2,390,516	-	nuoM	b2277	0.64	+0.0
2,390,522	-				-0.5
2,390,522	-	nuoM	b2277	0.65	-1.6
2,390,530	+	nuoM	b2277	0.65	+1.5
2,390,575	-	nuoM	b2277	0.68	-1.6
2,390,591	-	nuoM	b2277	0.69	-1.7
2,390,591	-	nuoM	b2277	0.69	-1.2
2,390,596	-	nuoM	b2277	0.69	-0.2
2,390,598	-	nuoM	b2277	0.70	-1.2
2,390,886	-	nuoM	b2277	0.88	-1.5
2,390,886	-	nuoM	b2277	0.88	-1.7
2,390,912	-				-0.1
2,390,948	+				-1.0
2,390,972	+				-1.1
2,391,053	+				-2.4
2,391,068	-				-2.9
2,391,102	+				+0.8
2,391,150	+				+0.6
2,391,153	+				-1.3
2,391,197	+				-0.2
2,391,238	-				-0.5
2,391,245	-				-0.4
2,391,248	+				-2.6
2,391,259	+				-0.0
2,391,278	-				+0.9
2,391,281	-				+0.8
2,391,281	-				-2.7
2,391,317	+				-3.0
2,391,372	-				-1.4
2,391,414	+				-1.4
2,391,414	+	nuoL	b2278	0.10	+1.1
2,391,419	+	nuoL	b2278	0.10	-0.9
2,391,439	-	nuoL	b2278	0.12	-1.5
2,391,505	-	nuoL	b2278	0.15	-1.4
2,391,505	-	nuoL	b2278	0.15	+0.0
2,391,517	+	nuoL	b2278	0.16	+1.8
2,391,567	-	nuoL	b2278	0.18	-0.5
2,391,785	+	nuoL	b2278	0.30	-0.1
2,391,795	+	nuoL	b2278	0.31	-2.6
2,391,809	+	nuoL	b2278	0.32	-2.8
2,391,809	+	nuoL	b2278	0.32	-0.1
2,391,921	-	nuoL	b2278	0.38	+0.7
2,391,985	-	nuoL	b2278	0.41	+1.2
2,392,049	-	nuoL	b2278	0.45	-1.3