Strain Fitness in Escherichia coli BW25113 around b2410

Experiment: Ying_sugar7 rep C; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyfeR and yfeH are separated by 89 nucleotidesyfeH and ypeB overlap by 4 nucleotidesypeB and ligA are separated by 1 nucleotides b2409: yfeR - predicted DNA-binding transcriptional regulator (NCBI), at 2,523,952 to 2,524,878 yfeR b2410: yfeH - predicted inner membrane protein (NCBI), at 2,524,968 to 2,525,966 yfeH b4546: ypeB - hypothetical protein (NCBI), at 2,525,963 to 2,526,181 ypeB b2411: ligA - NAD-dependent DNA ligase LigA (NCBI), at 2,526,183 to 2,528,198 ligA Position (kb) 2524 2525 2526Strain fitness (log2 ratio) -1 0 1at 2523.972 kb on - strandat 2524.005 kb on + strandat 2524.006 kb on + strandat 2524.006 kb on + strandat 2524.231 kb on - strand, within yfeRat 2524.422 kb on + strand, within yfeRat 2524.481 kb on - strand, within yfeRat 2524.614 kb on + strand, within yfeRat 2524.636 kb on - strand, within yfeRat 2524.636 kb on - strand, within yfeRat 2524.653 kb on - strand, within yfeRat 2524.653 kb on - strand, within yfeRat 2524.788 kb on + strandat 2524.794 kb on + strandat 2524.794 kb on + strandat 2524.802 kb on - strandat 2524.941 kb on - strandat 2524.941 kb on - strandat 2525.116 kb on + strand, within yfeHat 2525.248 kb on - strand, within yfeHat 2525.454 kb on - strand, within yfeHat 2525.454 kb on - strand, within yfeHat 2525.454 kb on - strand, within yfeHat 2525.454 kb on - strand, within yfeHat 2525.613 kb on + strand, within yfeHat 2525.709 kb on + strand, within yfeHat 2525.727 kb on - strand, within yfeHat 2525.727 kb on - strand, within yfeHat 2525.887 kb on + strandat 2525.942 kb on + strandat 2526.092 kb on + strand, within ypeB

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_sugar7 rep C; time point 1
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2,523,972 - +0.4
2,524,005 + +0.7
2,524,006 + +0.3
2,524,006 + +0.6
2,524,231 - yfeR b2409 0.30 +0.6
2,524,422 + yfeR b2409 0.51 +0.2
2,524,481 - yfeR b2409 0.57 +0.3
2,524,614 + yfeR b2409 0.71 -0.1
2,524,636 - yfeR b2409 0.74 +0.8
2,524,636 - yfeR b2409 0.74 -0.5
2,524,653 - yfeR b2409 0.76 -0.1
2,524,653 - yfeR b2409 0.76 +0.5
2,524,788 + -0.1
2,524,794 + +0.3
2,524,794 + -0.2
2,524,802 - +1.4
2,524,941 - +0.2
2,524,941 - -0.1
2,525,116 + yfeH b2410 0.15 +0.2
2,525,248 - yfeH b2410 0.28 +0.6
2,525,454 - yfeH b2410 0.49 +0.5
2,525,454 - yfeH b2410 0.49 +0.2
2,525,454 - yfeH b2410 0.49 -0.6
2,525,454 - yfeH b2410 0.49 +0.4
2,525,613 + yfeH b2410 0.65 -0.1
2,525,709 + yfeH b2410 0.74 +0.5
2,525,727 - yfeH b2410 0.76 +0.6
2,525,727 - yfeH b2410 0.76 +1.1
2,525,887 + -0.5
2,525,942 + +0.6
2,526,092 + ypeB b4546 0.59 -0.6

Or see this region's nucleotide sequence