Strain Fitness in Escherichia coli BW25113 around b0686

Experiment: Ying_sugar7 rep C; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfldA and ybfE are separated by 139 nucleotidesybfE and ybfF are separated by 139 nucleotidesybfF and seqA are separated by 184 nucleotidesseqA and pgm are separated by 25 nucleotides b0684: fldA - flavodoxin 1 (NCBI), at 710,158 to 710,688 fldA b0685: ybfE - orf, hypothetical protein (VIMSS), at 710,828 to 711,121 ybfE b0686: ybfF - hypothetical protein (NCBI), at 711,261 to 712,025 ybfF b0687: seqA - regulatory protein for replication initiation (NCBI), at 712,210 to 712,755 seqA b0688: pgm - phosphoglucomutase (NCBI), at 712,781 to 714,421 pgm Position (kb) 711 712 713Strain fitness (log2 ratio) -1 0 1at 710.751 kb on + strandat 710.780 kb on - strandat 710.780 kb on - strandat 710.787 kb on + strandat 710.798 kb on + strandat 710.805 kb on - strandat 710.805 kb on - strandat 710.807 kb on + strandat 710.901 kb on + strand, within ybfEat 710.924 kb on + strand, within ybfEat 710.924 kb on + strand, within ybfEat 711.159 kb on - strandat 711.400 kb on - strand, within ybfFat 711.569 kb on + strand, within ybfFat 711.668 kb on - strand, within ybfFat 711.685 kb on + strand, within ybfFat 711.728 kb on - strand, within ybfFat 711.730 kb on - strand, within ybfFat 711.730 kb on - strand, within ybfFat 711.803 kb on - strand, within ybfFat 711.803 kb on - strand, within ybfFat 711.900 kb on - strand, within ybfFat 711.923 kb on + strand, within ybfFat 711.923 kb on + strand, within ybfFat 711.933 kb on + strand, within ybfFat 712.060 kb on + strandat 712.077 kb on + strandat 712.124 kb on + strandat 712.202 kb on + strandat 712.758 kb on - strandat 712.814 kb on - strandat 712.937 kb on + strandat 712.978 kb on + strand, within pgmat 713.023 kb on + strand, within pgm

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_sugar7 rep C; time point 1
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710,751 + -0.2
710,780 - -0.4
710,780 - -0.6
710,787 + -0.4
710,798 + +0.1
710,805 - +0.1
710,805 - -0.4
710,807 + +0.5
710,901 + ybfE b0685 0.25 +0.3
710,924 + ybfE b0685 0.33 +0.3
710,924 + ybfE b0685 0.33 +0.7
711,159 - +0.3
711,400 - ybfF b0686 0.18 +0.3
711,569 + ybfF b0686 0.40 +0.8
711,668 - ybfF b0686 0.53 +0.4
711,685 + ybfF b0686 0.55 -0.3
711,728 - ybfF b0686 0.61 +0.2
711,730 - ybfF b0686 0.61 -0.1
711,730 - ybfF b0686 0.61 +0.3
711,803 - ybfF b0686 0.71 -0.2
711,803 - ybfF b0686 0.71 +0.0
711,900 - ybfF b0686 0.84 +0.3
711,923 + ybfF b0686 0.87 +0.4
711,923 + ybfF b0686 0.87 +1.3
711,933 + ybfF b0686 0.88 +0.6
712,060 + +0.1
712,077 + -0.4
712,124 + +0.2
712,202 + -0.1
712,758 - +0.3
712,814 - -1.3
712,937 + +0.3
712,978 + pgm b0688 0.12 -1.6
713,023 + pgm b0688 0.15 -0.2

Or see this region's nucleotide sequence