Strain Fitness in Escherichia coli BW25113 around b0641

Experiment: Ying_sugar7 rep C; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntholA and rlpB overlap by 1 nucleotidesrlpB and leuS are separated by 14 nucleotides b0640: holA - DNA polymerase III subunit delta (NCBI), at 669,797 to 670,828 holA b0641: rlpB - minor lipoprotein (NCBI), at 670,828 to 671,409 rlpB b0642: leuS - leucyl-tRNA synthetase (NCBI), at 671,424 to 674,006 leuS Position (kb) 670 671 672Strain fitness (log2 ratio) -2 -1 0 1at 669.852 kb on + strandat 670.785 kb on + strandat 670.837 kb on - strandat 670.880 kb on - strandat 670.915 kb on - strand, within rlpBat 670.922 kb on - strand, within rlpBat 671.394 kb on - strandat 671.404 kb on - strandat 671.404 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_sugar7 rep C; time point 1
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669,852 + -0.3
670,785 + -0.5
670,837 - +0.2
670,880 - -0.2
670,915 - rlpB b0641 0.15 +0.2
670,922 - rlpB b0641 0.16 +0.5
671,394 - -2.2
671,404 - +0.1
671,404 - -0.0

Or see this region's nucleotide sequence