Experiment: Ying_sugar7 rep B; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt exbD and exbB are separated by 6 nucleotides exbB and metC are separated by 251 nucleotides metC and yghB are separated by 139 nucleotides yghB and yqhC are separated by 39 nucleotides
b3005: exbD - membrane spanning protein in TonB-ExbB-ExbD complex (NCBI), at 3,148,840 to 3,149,265
exbD
b3006: exbB - membrane spanning protein in TonB-ExbB-ExbD complex (NCBI), at 3,149,272 to 3,150,006
exbB
b3008: metC - cystathionine beta-lyase (NCBI), at 3,150,258 to 3,151,445
metC
b3009: yghB - conserved inner membrane protein (NCBI), at 3,151,585 to 3,152,244
yghB
b3010: yqhC - putative ARAC-type regulatory protein (VIMSS), at 3,152,284 to 3,153,240
yqhC
Position (kb)
3150
3151
3152 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3149.377 kb on + strand, within exbB at 3149.377 kb on + strand, within exbB at 3149.380 kb on + strand, within exbB at 3149.396 kb on - strand, within exbB at 3149.448 kb on - strand, within exbB at 3149.501 kb on + strand, within exbB at 3149.508 kb on + strand, within exbB at 3149.543 kb on - strand, within exbB at 3149.553 kb on - strand, within exbB at 3149.554 kb on + strand, within exbB at 3149.564 kb on + strand, within exbB at 3149.659 kb on + strand, within exbB at 3149.682 kb on - strand, within exbB at 3149.682 kb on - strand, within exbB at 3149.690 kb on - strand, within exbB at 3149.707 kb on + strand, within exbB at 3149.710 kb on - strand, within exbB at 3149.718 kb on + strand, within exbB at 3149.803 kb on - strand, within exbB at 3149.803 kb on - strand, within exbB at 3149.866 kb on + strand, within exbB at 3149.956 kb on + strand at 3149.956 kb on + strand at 3149.956 kb on + strand at 3149.983 kb on + strand at 3150.016 kb on - strand at 3150.257 kb on - strand at 3150.288 kb on - strand at 3150.292 kb on + strand at 3150.300 kb on - strand at 3150.300 kb on - strand at 3150.337 kb on - strand at 3150.378 kb on - strand, within metC at 3150.421 kb on + strand, within metC at 3150.421 kb on + strand, within metC at 3150.514 kb on - strand, within metC at 3150.575 kb on + strand, within metC at 3150.577 kb on - strand, within metC at 3150.583 kb on - strand, within metC at 3150.640 kb on - strand, within metC at 3150.654 kb on + strand, within metC at 3150.669 kb on + strand, within metC at 3150.706 kb on - strand, within metC at 3150.791 kb on - strand, within metC at 3150.820 kb on + strand, within metC at 3150.820 kb on + strand, within metC at 3150.912 kb on + strand, within metC at 3150.914 kb on + strand, within metC at 3150.915 kb on + strand, within metC at 3150.932 kb on - strand, within metC at 3151.015 kb on + strand at 3151.015 kb on + strand, within metC at 3151.028 kb on + strand, within metC at 3151.038 kb on - strand, within metC at 3151.038 kb on - strand, within metC at 3151.074 kb on - strand, within metC at 3151.074 kb on - strand, within metC at 3151.120 kb on + strand, within metC at 3151.125 kb on + strand, within metC at 3151.138 kb on - strand, within metC at 3151.216 kb on + strand, within metC at 3151.216 kb on + strand, within metC at 3151.237 kb on + strand, within metC at 3151.274 kb on - strand, within metC at 3151.376 kb on + strand at 3151.655 kb on - strand, within yghB at 3151.859 kb on + strand, within yghB at 3151.859 kb on + strand, within yghB at 3151.867 kb on - strand, within yghB at 3151.889 kb on + strand, within yghB at 3151.889 kb on + strand, within yghB at 3152.046 kb on + strand, within yghB at 3152.046 kb on + strand, within yghB at 3152.076 kb on + strand, within yghB at 3152.084 kb on - strand, within yghB at 3152.101 kb on - strand, within yghB at 3152.101 kb on - strand, within yghB at 3152.130 kb on + strand, within yghB at 3152.133 kb on + strand, within yghB at 3152.152 kb on - strand, within yghB at 3152.153 kb on - strand, within yghB at 3152.186 kb on + strand at 3152.256 kb on + strand at 3152.256 kb on + strand at 3152.280 kb on + strand at 3152.290 kb on + strand at 3152.290 kb on + strand at 3152.372 kb on + strand at 3152.377 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep B; time point 1 remove 3,149,377 + exbB b3006 0.14 -0.6 3,149,377 + exbB b3006 0.14 -0.1 3,149,380 + exbB b3006 0.15 -0.6 3,149,396 - exbB b3006 0.17 -0.2 3,149,448 - exbB b3006 0.24 -0.6 3,149,501 + exbB b3006 0.31 -0.4 3,149,508 + exbB b3006 0.32 +0.5 3,149,543 - exbB b3006 0.37 -0.3 3,149,553 - exbB b3006 0.38 +0.0 3,149,554 + exbB b3006 0.38 +0.5 3,149,564 + exbB b3006 0.40 +0.0 3,149,659 + exbB b3006 0.53 -0.3 3,149,682 - exbB b3006 0.56 -0.4 3,149,682 - exbB b3006 0.56 +0.0 3,149,690 - exbB b3006 0.57 +0.2 3,149,707 + exbB b3006 0.59 -0.3 3,149,710 - exbB b3006 0.60 -0.1 3,149,718 + exbB b3006 0.61 -0.7 3,149,803 - exbB b3006 0.72 -0.7 3,149,803 - exbB b3006 0.72 -0.1 3,149,866 + exbB b3006 0.81 -1.1 3,149,956 + +0.1 3,149,956 + +0.0 3,149,956 + -0.4 3,149,983 + +0.6 3,150,016 - -0.3 3,150,257 - -0.1 3,150,288 - -3.3 3,150,292 + -4.5 3,150,300 - -2.8 3,150,300 - -1.6 3,150,337 - -3.7 3,150,378 - metC b3008 0.10 -2.7 3,150,421 + metC b3008 0.14 -2.1 3,150,421 + metC b3008 0.14 -4.4 3,150,514 - metC b3008 0.22 -2.7 3,150,575 + metC b3008 0.27 -5.1 3,150,577 - metC b3008 0.27 -3.0 3,150,583 - metC b3008 0.27 -2.0 3,150,640 - metC b3008 0.32 -3.7 3,150,654 + metC b3008 0.33 -3.6 3,150,669 + metC b3008 0.35 -3.2 3,150,706 - metC b3008 0.38 -4.3 3,150,791 - metC b3008 0.45 -3.1 3,150,820 + metC b3008 0.47 -3.7 3,150,820 + metC b3008 0.47 -3.6 3,150,912 + metC b3008 0.55 -3.7 3,150,914 + metC b3008 0.55 -3.0 3,150,915 + metC b3008 0.55 -2.6 3,150,932 - metC b3008 0.57 -3.3 3,151,015 + -2.0 3,151,015 + metC b3008 0.64 -0.7 3,151,028 + metC b3008 0.65 -3.6 3,151,038 - metC b3008 0.66 -2.6 3,151,038 - metC b3008 0.66 -3.6 3,151,074 - metC b3008 0.69 -3.2 3,151,074 - metC b3008 0.69 -4.3 3,151,120 + metC b3008 0.73 -2.8 3,151,125 + metC b3008 0.73 -0.5 3,151,138 - metC b3008 0.74 -3.7 3,151,216 + metC b3008 0.81 -3.3 3,151,216 + metC b3008 0.81 -2.6 3,151,237 + metC b3008 0.82 -2.9 3,151,274 - metC b3008 0.86 -3.1 3,151,376 + -4.6 3,151,655 - yghB b3009 0.11 +0.5 3,151,859 + yghB b3009 0.42 -0.0 3,151,859 + yghB b3009 0.42 +0.3 3,151,867 - yghB b3009 0.43 +0.8 3,151,889 + yghB b3009 0.46 +0.8 3,151,889 + yghB b3009 0.46 -0.4 3,152,046 + yghB b3009 0.70 +1.0 3,152,046 + yghB b3009 0.70 +0.2 3,152,076 + yghB b3009 0.74 -0.1 3,152,084 - yghB b3009 0.76 +0.2 3,152,101 - yghB b3009 0.78 +0.3 3,152,101 - yghB b3009 0.78 +0.6 3,152,130 + yghB b3009 0.83 +0.2 3,152,133 + yghB b3009 0.83 +0.3 3,152,152 - yghB b3009 0.86 -0.0 3,152,153 - yghB b3009 0.86 +0.1 3,152,186 + +0.2 3,152,256 + -0.1 3,152,256 + +0.0 3,152,280 + -0.6 3,152,290 + +1.0 3,152,290 + -0.0 3,152,372 + -0.4 3,152,377 + -0.5
Or see this region's nucleotide sequence