Strain Fitness in Escherichia coli BW25113 around b2052

Experiment: Ying_sugar7 rep B; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwcaI and wcaH overlap by 4 nucleotideswcaH and fcl are separated by 2 nucleotidesfcl and gmd are separated by 2 nucleotides b2050: wcaI - predicted glycosyl transferase (NCBI), at 2,122,547 to 2,123,770 wcaI b2051: wcaH - GDP-mannose mannosyl hydrolase (VIMSS), at 2,123,767 to 2,124,246 wcaH b2052: fcl - bifunctional GDP-fucose synthetase: GDP-4-dehydro-6-deoxy-D-mannose epimerase/ GDP-4-dehydro-6-L-deoxygalactose reductase (NCBI), at 2,124,249 to 2,125,214 fcl b2053: gmd - GDP-D-mannose dehydratase, NAD(P)-binding (NCBI), at 2,125,217 to 2,126,338 gmd Position (kb) 2124 2125 2126Strain fitness (log2 ratio) -1 0 1 2at 2123.290 kb on + strand, within wcaIat 2123.290 kb on + strand, within wcaIat 2123.479 kb on + strand, within wcaIat 2123.610 kb on + strand, within wcaIat 2123.668 kb on + strandat 2123.822 kb on + strand, within wcaHat 2124.177 kb on - strand, within wcaHat 2124.282 kb on + strandat 2124.282 kb on + strandat 2124.465 kb on + strand, within fclat 2124.601 kb on + strand, within fclat 2124.630 kb on + strand, within fclat 2124.630 kb on + strand, within fclat 2124.722 kb on - strand, within fclat 2124.813 kb on - strand, within fclat 2124.848 kb on - strand, within fclat 2124.896 kb on + strand, within fclat 2124.951 kb on + strand, within fclat 2124.951 kb on + strand, within fclat 2125.046 kb on - strand, within fclat 2125.113 kb on + strand, within fclat 2125.189 kb on + strandat 2125.221 kb on - strandat 2125.556 kb on + strand, within gmdat 2125.566 kb on + strand, within gmdat 2125.570 kb on + strand, within gmdat 2125.570 kb on + strand, within gmdat 2125.643 kb on + strand, within gmdat 2125.706 kb on + strand, within gmdat 2125.707 kb on - strand, within gmdat 2125.797 kb on + strand, within gmdat 2125.797 kb on + strand, within gmdat 2125.828 kb on + strand, within gmdat 2125.887 kb on + strand, within gmdat 2125.941 kb on - strand, within gmdat 2126.048 kb on + strand, within gmdat 2126.090 kb on - strand, within gmdat 2126.093 kb on - strand, within gmdat 2126.151 kb on + strand, within gmdat 2126.181 kb on - strand, within gmd

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_sugar7 rep B; time point 1
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2,123,290 + wcaI b2050 0.61 -1.1
2,123,290 + wcaI b2050 0.61 +0.3
2,123,479 + wcaI b2050 0.76 +0.3
2,123,610 + wcaI b2050 0.87 -0.3
2,123,668 + -0.1
2,123,822 + wcaH b2051 0.11 +0.4
2,124,177 - wcaH b2051 0.85 -0.7
2,124,282 + +0.3
2,124,282 + -0.2
2,124,465 + fcl b2052 0.22 -0.3
2,124,601 + fcl b2052 0.36 +0.2
2,124,630 + fcl b2052 0.39 +0.7
2,124,630 + fcl b2052 0.39 +0.5
2,124,722 - fcl b2052 0.49 +0.5
2,124,813 - fcl b2052 0.58 +0.3
2,124,848 - fcl b2052 0.62 -0.6
2,124,896 + fcl b2052 0.67 -0.4
2,124,951 + fcl b2052 0.73 +0.4
2,124,951 + fcl b2052 0.73 +0.5
2,125,046 - fcl b2052 0.83 +2.1
2,125,113 + fcl b2052 0.89 +0.2
2,125,189 + -0.6
2,125,221 - -0.0
2,125,556 + gmd b2053 0.30 +0.3
2,125,566 + gmd b2053 0.31 +0.3
2,125,570 + gmd b2053 0.31 -0.3
2,125,570 + gmd b2053 0.31 -0.4
2,125,643 + gmd b2053 0.38 +0.2
2,125,706 + gmd b2053 0.44 +0.2
2,125,707 - gmd b2053 0.44 +0.2
2,125,797 + gmd b2053 0.52 +1.2
2,125,797 + gmd b2053 0.52 +1.1
2,125,828 + gmd b2053 0.54 +0.1
2,125,887 + gmd b2053 0.60 -0.0
2,125,941 - gmd b2053 0.65 +0.8
2,126,048 + gmd b2053 0.74 +0.7
2,126,090 - gmd b2053 0.78 +0.4
2,126,093 - gmd b2053 0.78 -0.0
2,126,151 + gmd b2053 0.83 +0.2
2,126,181 - gmd b2053 0.86 -0.3

Or see this region's nucleotide sequence