Experiment: Ying_sugar7 rep B; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dinB and yafN are separated by 51 nucleotides yafN and yafO are separated by 2 nucleotides yafO and yafP are separated by 9 nucleotides
b0231: dinB - DNA polymerase IV (NCBI), at 250,898 to 251,953
dinB
b0232: yafN - predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI), at 252,005 to 252,298
yafN
b0233: yafO - predicted toxin of the YafO-YafN toxin-antitoxin system (NCBI), at 252,301 to 252,699
yafO
b0234: yafP - predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI), at 252,709 to 253,161
yafP
Position (kb) 252
253 Strain fitness (log2 ratio) -1
0
1
2
3 at 251.129 kb on - strand at 251.129 kb on - strand, within dinB at 251.214 kb on + strand, within dinB at 251.240 kb on + strand, within dinB at 251.240 kb on + strand, within dinB at 251.240 kb on - strand, within dinB at 251.240 kb on - strand, within dinB at 251.255 kb on - strand, within dinB at 251.499 kb on - strand, within dinB at 251.499 kb on - strand, within dinB at 251.579 kb on - strand, within dinB at 251.662 kb on - strand, within dinB at 251.669 kb on + strand, within dinB at 251.803 kb on + strand, within dinB at 251.803 kb on + strand, within dinB at 251.818 kb on + strand, within dinB at 251.880 kb on + strand at 251.901 kb on + strand at 251.909 kb on - strand at 251.924 kb on + strand at 251.965 kb on - strand at 252.003 kb on - strand at 252.137 kb on - strand, within yafN at 252.155 kb on - strand, within yafN at 252.288 kb on + strand at 252.331 kb on + strand at 252.331 kb on + strand at 252.341 kb on - strand, within yafO at 252.346 kb on + strand, within yafO at 252.368 kb on - strand, within yafO at 252.368 kb on - strand, within yafO at 252.380 kb on + strand, within yafO at 252.381 kb on + strand, within yafO at 252.419 kb on + strand, within yafO at 252.427 kb on + strand, within yafO at 252.427 kb on + strand, within yafO at 252.440 kb on + strand, within yafO at 252.440 kb on + strand, within yafO at 252.440 kb on + strand, within yafO at 252.440 kb on + strand, within yafO at 252.440 kb on + strand, within yafO at 252.443 kb on - strand, within yafO at 252.448 kb on - strand, within yafO at 252.475 kb on + strand, within yafO at 252.522 kb on - strand, within yafO at 252.522 kb on - strand, within yafO at 252.534 kb on - strand, within yafO at 252.538 kb on + strand, within yafO at 252.538 kb on + strand, within yafO at 252.563 kb on + strand, within yafO at 252.566 kb on - strand, within yafO at 252.574 kb on - strand, within yafO at 252.574 kb on - strand, within yafO at 252.592 kb on - strand, within yafO at 252.594 kb on - strand, within yafO at 252.597 kb on + strand, within yafO at 252.610 kb on - strand, within yafO at 252.614 kb on + strand, within yafO at 252.622 kb on - strand, within yafO at 252.628 kb on + strand, within yafO at 252.671 kb on + strand at 252.671 kb on + strand at 252.739 kb on - strand at 252.739 kb on - strand at 252.779 kb on - strand, within yafP at 252.781 kb on + strand, within yafP at 252.848 kb on + strand, within yafP at 252.849 kb on + strand, within yafP at 252.879 kb on + strand, within yafP at 252.896 kb on + strand, within yafP at 252.936 kb on + strand, within yafP at 252.973 kb on + strand, within yafP at 252.974 kb on + strand, within yafP at 253.041 kb on + strand, within yafP at 253.077 kb on + strand, within yafP at 253.087 kb on + strand, within yafP at 253.087 kb on + strand, within yafP at 253.087 kb on + strand, within yafP at 253.102 kb on + strand, within yafP at 253.188 kb on + strand at 253.198 kb on - strand at 253.220 kb on + strand at 253.222 kb on + strand at 253.222 kb on - strand at 253.272 kb on - strand at 253.275 kb on + strand at 253.283 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep B; time point 1 remove 251,129 - -0.2 251,129 - dinB b0231 0.22 -0.4 251,214 + dinB b0231 0.30 -0.2 251,240 + dinB b0231 0.32 -0.3 251,240 + dinB b0231 0.32 -1.5 251,240 - dinB b0231 0.32 +0.1 251,240 - dinB b0231 0.32 +0.4 251,255 - dinB b0231 0.34 +0.2 251,499 - dinB b0231 0.57 -0.5 251,499 - dinB b0231 0.57 -0.2 251,579 - dinB b0231 0.64 +0.3 251,662 - dinB b0231 0.72 +0.3 251,669 + dinB b0231 0.73 +0.4 251,803 + dinB b0231 0.86 -0.5 251,803 + dinB b0231 0.86 +0.4 251,818 + dinB b0231 0.87 -0.2 251,880 + -0.5 251,901 + +0.1 251,909 - +0.3 251,924 + -0.0 251,965 - +2.5 252,003 - +0.8 252,137 - yafN b0232 0.45 -1.6 252,155 - yafN b0232 0.51 +1.8 252,288 + -0.2 252,331 + +0.5 252,331 + -0.5 252,341 - yafO b0233 0.10 -1.1 252,346 + yafO b0233 0.11 -0.4 252,368 - yafO b0233 0.17 +2.5 252,368 - yafO b0233 0.17 +2.5 252,380 + yafO b0233 0.20 +0.0 252,381 + yafO b0233 0.20 -1.1 252,419 + yafO b0233 0.30 +0.3 252,427 + yafO b0233 0.32 +1.9 252,427 + yafO b0233 0.32 +2.5 252,440 + yafO b0233 0.35 +1.3 252,440 + yafO b0233 0.35 -1.0 252,440 + yafO b0233 0.35 -0.0 252,440 + yafO b0233 0.35 -0.6 252,440 + yafO b0233 0.35 +0.7 252,443 - yafO b0233 0.36 +1.5 252,448 - yafO b0233 0.37 +2.5 252,475 + yafO b0233 0.44 +2.2 252,522 - yafO b0233 0.55 -0.6 252,522 - yafO b0233 0.55 +0.4 252,534 - yafO b0233 0.58 -0.7 252,538 + yafO b0233 0.59 -1.4 252,538 + yafO b0233 0.59 -0.2 252,563 + yafO b0233 0.66 -0.1 252,566 - yafO b0233 0.66 +1.5 252,574 - yafO b0233 0.68 -0.1 252,574 - yafO b0233 0.68 -0.3 252,592 - yafO b0233 0.73 +1.5 252,594 - yafO b0233 0.73 +2.5 252,597 + yafO b0233 0.74 +0.1 252,610 - yafO b0233 0.77 +1.5 252,614 + yafO b0233 0.78 +0.9 252,622 - yafO b0233 0.80 +2.5 252,628 + yafO b0233 0.82 +0.5 252,671 + +0.7 252,671 + +0.8 252,739 - +2.5 252,739 - +1.9 252,779 - yafP b0234 0.15 +1.5 252,781 + yafP b0234 0.16 +2.5 252,848 + yafP b0234 0.31 +0.2 252,849 + yafP b0234 0.31 +2.5 252,879 + yafP b0234 0.38 +0.9 252,896 + yafP b0234 0.41 +1.9 252,936 + yafP b0234 0.50 -0.1 252,973 + yafP b0234 0.58 +2.5 252,974 + yafP b0234 0.58 +2.5 253,041 + yafP b0234 0.73 -1.6 253,077 + yafP b0234 0.81 +0.2 253,087 + yafP b0234 0.83 -0.6 253,087 + yafP b0234 0.83 -0.3 253,087 + yafP b0234 0.83 +0.5 253,102 + yafP b0234 0.87 +3.5 253,188 + +1.5 253,198 - +0.5 253,220 + -0.1 253,222 + +0.9 253,222 - +2.5 253,272 - +0.9 253,275 + +1.5 253,283 - +1.5
Or see this region's nucleotide sequence