Experiment: Ying_sugar7 rep B; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tilS and yaeO are separated by 48 nucleotides yaeO and yaeP overlap by 8 nucleotides yaeP and yaeQ are separated by 165 nucleotides yaeQ and yaeJ overlap by 4 nucleotides yaeJ and nlpE are separated by 13 nucleotides
b0188: tilS - tRNA(Ile)-lysidine synthetase (NCBI), at 212,331 to 213,629
tilS
b0189: yaeO - orf, hypothetical protein (VIMSS), at 213,678 to 213,932
yaeO
b4406: yaeP - hypothetical protein (NCBI), at 213,925 to 214,125
yaeP
b0190: yaeQ - hypothetical protein (NCBI), at 214,291 to 214,836
yaeQ
b0191: yaeJ - hypothetical protein (NCBI), at 214,833 to 215,255
yaeJ
b0192: nlpE - lipoprotein involved with copper homeostasis and adhesion (NCBI), at 215,269 to 215,979
nlpE
Position (kb) 214
215 Strain fitness (log2 ratio) -2
-1
0
1 at 213.686 kb on + strand at 213.686 kb on + strand at 213.716 kb on + strand, within yaeO at 213.716 kb on + strand, within yaeO at 213.716 kb on + strand, within yaeO at 213.716 kb on + strand, within yaeO at 213.783 kb on + strand, within yaeO at 213.880 kb on - strand, within yaeO at 213.880 kb on - strand, within yaeO at 213.913 kb on + strand at 213.913 kb on + strand at 213.956 kb on - strand, within yaeP at 213.956 kb on - strand, within yaeP at 214.040 kb on - strand, within yaeP at 214.130 kb on + strand at 214.142 kb on + strand at 214.166 kb on + strand at 214.174 kb on - strand at 214.174 kb on - strand at 214.216 kb on - strand at 214.369 kb on - strand, within yaeQ at 214.391 kb on - strand, within yaeQ at 214.404 kb on - strand, within yaeQ at 214.419 kb on - strand, within yaeQ at 214.468 kb on + strand, within yaeQ at 214.487 kb on + strand, within yaeQ at 214.487 kb on + strand, within yaeQ at 214.542 kb on + strand, within yaeQ at 214.572 kb on + strand, within yaeQ at 214.574 kb on + strand, within yaeQ at 214.600 kb on - strand, within yaeQ at 214.606 kb on + strand, within yaeQ at 214.606 kb on + strand, within yaeQ at 214.764 kb on - strand, within yaeQ at 214.773 kb on - strand, within yaeQ at 214.808 kb on + strand at 214.825 kb on - strand at 214.836 kb on - strand at 214.872 kb on + strand at 214.874 kb on + strand at 214.919 kb on + strand, within yaeJ at 215.045 kb on + strand, within yaeJ at 215.045 kb on + strand, within yaeJ at 215.091 kb on + strand, within yaeJ at 215.123 kb on + strand, within yaeJ at 215.123 kb on + strand, within yaeJ at 215.299 kb on + strand at 215.372 kb on + strand, within nlpE at 215.378 kb on + strand, within nlpE at 215.410 kb on + strand, within nlpE at 215.476 kb on - strand, within nlpE at 215.498 kb on - strand, within nlpE at 215.498 kb on - strand, within nlpE at 215.558 kb on + strand, within nlpE at 215.582 kb on - strand, within nlpE at 215.621 kb on + strand, within nlpE at 215.635 kb on - strand, within nlpE at 215.635 kb on - strand, within nlpE at 215.694 kb on + strand, within nlpE at 215.756 kb on + strand, within nlpE at 215.816 kb on + strand, within nlpE at 215.816 kb on + strand, within nlpE at 215.824 kb on - strand, within nlpE
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep B; time point 1 remove 213,686 + +0.1 213,686 + -0.2 213,716 + yaeO b0189 0.15 -0.6 213,716 + yaeO b0189 0.15 -0.2 213,716 + yaeO b0189 0.15 -0.3 213,716 + yaeO b0189 0.15 +0.1 213,783 + yaeO b0189 0.41 -0.1 213,880 - yaeO b0189 0.79 -0.7 213,880 - yaeO b0189 0.79 -0.5 213,913 + -2.3 213,913 + +0.4 213,956 - yaeP b4406 0.15 +0.3 213,956 - yaeP b4406 0.15 +0.1 214,040 - yaeP b4406 0.57 +0.5 214,130 + -0.5 214,142 + -0.2 214,166 + -1.2 214,174 - +0.8 214,174 - +0.9 214,216 - +0.6 214,369 - yaeQ b0190 0.14 +0.7 214,391 - yaeQ b0190 0.18 +1.0 214,404 - yaeQ b0190 0.21 +0.4 214,419 - yaeQ b0190 0.23 +0.2 214,468 + yaeQ b0190 0.32 +0.3 214,487 + yaeQ b0190 0.36 +0.0 214,487 + yaeQ b0190 0.36 +0.4 214,542 + yaeQ b0190 0.46 +0.1 214,572 + yaeQ b0190 0.51 +0.2 214,574 + yaeQ b0190 0.52 +0.1 214,600 - yaeQ b0190 0.57 +0.4 214,606 + yaeQ b0190 0.58 +0.4 214,606 + yaeQ b0190 0.58 +0.3 214,764 - yaeQ b0190 0.87 +0.0 214,773 - yaeQ b0190 0.88 +0.4 214,808 + +0.4 214,825 - +0.3 214,836 - +0.7 214,872 + -0.3 214,874 + +0.1 214,919 + yaeJ b0191 0.20 +0.5 215,045 + yaeJ b0191 0.50 +0.9 215,045 + yaeJ b0191 0.50 -0.1 215,091 + yaeJ b0191 0.61 -0.5 215,123 + yaeJ b0191 0.69 -0.5 215,123 + yaeJ b0191 0.69 -0.2 215,299 + -0.1 215,372 + nlpE b0192 0.14 +0.5 215,378 + nlpE b0192 0.15 +0.1 215,410 + nlpE b0192 0.20 -0.2 215,476 - nlpE b0192 0.29 +0.1 215,498 - nlpE b0192 0.32 +0.1 215,498 - nlpE b0192 0.32 +0.3 215,558 + nlpE b0192 0.41 +0.2 215,582 - nlpE b0192 0.44 +0.0 215,621 + nlpE b0192 0.50 +0.4 215,635 - nlpE b0192 0.51 +1.4 215,635 - nlpE b0192 0.51 +1.5 215,694 + nlpE b0192 0.60 -0.6 215,756 + nlpE b0192 0.68 -0.4 215,816 + nlpE b0192 0.77 -0.4 215,816 + nlpE b0192 0.77 +0.9 215,824 - nlpE b0192 0.78 -0.1
Or see this region's nucleotide sequence