Experiment: Ying_sugar7 rep A; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisC and hisB overlap by 1 nucleotides hisB and hisH overlap by 1 nucleotides hisH and hisA overlap by 1 nucleotides
b2021: hisC - histidinol-phosphate aminotransferase (NCBI), at 2,090,422 to 2,091,492
hisC
b2022: hisB - imidazoleglycerolphosphate dehydratase and histidinol-phosphate phosphatase (VIMSS), at 2,091,492 to 2,092,559
hisB
b2023: hisH - imidazole glycerol phosphate synthase subunit HisH (NCBI), at 2,092,559 to 2,093,149
hisH
b2024: hisA - N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS), at 2,093,149 to 2,093,886
hisA
Position (kb)
2091
2092
2093 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2090.721 kb on - strand, within hisC at 2090.808 kb on - strand, within hisC at 2090.932 kb on + strand, within hisC at 2090.932 kb on + strand, within hisC at 2090.938 kb on + strand, within hisC at 2091.102 kb on + strand, within hisC at 2091.202 kb on - strand, within hisC at 2091.202 kb on - strand, within hisC at 2091.274 kb on + strand, within hisC at 2091.351 kb on + strand, within hisC at 2091.593 kb on - strand at 2091.593 kb on - strand at 2091.619 kb on - strand, within hisB at 2091.659 kb on + strand, within hisB at 2091.985 kb on - strand, within hisB at 2092.013 kb on - strand, within hisB at 2092.013 kb on - strand, within hisB at 2092.070 kb on + strand, within hisB at 2092.080 kb on + strand, within hisB at 2092.114 kb on - strand, within hisB at 2092.160 kb on + strand, within hisB at 2092.171 kb on - strand, within hisB at 2092.193 kb on - strand, within hisB at 2092.193 kb on - strand, within hisB at 2092.355 kb on + strand, within hisB at 2092.355 kb on + strand, within hisB at 2092.359 kb on - strand, within hisB at 2092.406 kb on + strand, within hisB at 2092.406 kb on + strand, within hisB at 2092.412 kb on + strand, within hisB at 2092.412 kb on + strand, within hisB at 2092.464 kb on + strand at 2092.533 kb on - strand at 2092.549 kb on - strand at 2092.752 kb on - strand, within hisH at 2092.772 kb on - strand, within hisH at 2092.907 kb on + strand, within hisH at 2093.026 kb on + strand, within hisH at 2093.026 kb on + strand, within hisH at 2093.071 kb on + strand, within hisH at 2093.071 kb on + strand, within hisH at 2093.079 kb on - strand, within hisH at 2093.079 kb on - strand, within hisH at 2093.079 kb on - strand, within hisH at 2093.079 kb on - strand, within hisH at 2093.094 kb on - strand at 2093.183 kb on + strand at 2093.183 kb on + strand at 2093.454 kb on - strand, within hisA at 2093.510 kb on - strand, within hisA
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep A; time point 1 remove 2,090,721 - hisC b2021 0.28 -3.7 2,090,808 - hisC b2021 0.36 -4.9 2,090,932 + hisC b2021 0.48 -4.3 2,090,932 + hisC b2021 0.48 -5.0 2,090,938 + hisC b2021 0.48 -5.1 2,091,102 + hisC b2021 0.63 -4.6 2,091,202 - hisC b2021 0.73 -4.0 2,091,202 - hisC b2021 0.73 -0.6 2,091,274 + hisC b2021 0.80 -4.2 2,091,351 + hisC b2021 0.87 -3.0 2,091,593 - -2.3 2,091,593 - -1.5 2,091,619 - hisB b2022 0.12 -4.1 2,091,659 + hisB b2022 0.16 -5.1 2,091,985 - hisB b2022 0.46 -3.5 2,092,013 - hisB b2022 0.49 -2.6 2,092,013 - hisB b2022 0.49 -5.6 2,092,070 + hisB b2022 0.54 -5.8 2,092,080 + hisB b2022 0.55 -3.9 2,092,114 - hisB b2022 0.58 -1.5 2,092,160 + hisB b2022 0.63 -4.4 2,092,171 - hisB b2022 0.64 -2.7 2,092,193 - hisB b2022 0.66 -4.5 2,092,193 - hisB b2022 0.66 -2.6 2,092,355 + hisB b2022 0.81 -3.0 2,092,355 + hisB b2022 0.81 -4.5 2,092,359 - hisB b2022 0.81 -3.3 2,092,406 + hisB b2022 0.86 -1.7 2,092,406 + hisB b2022 0.86 -3.6 2,092,412 + hisB b2022 0.86 -3.6 2,092,412 + hisB b2022 0.86 -1.7 2,092,464 + -2.7 2,092,533 - -4.4 2,092,549 - -1.0 2,092,752 - hisH b2023 0.33 -4.0 2,092,772 - hisH b2023 0.36 -3.0 2,092,907 + hisH b2023 0.59 -3.6 2,093,026 + hisH b2023 0.79 -3.4 2,093,026 + hisH b2023 0.79 -4.5 2,093,071 + hisH b2023 0.87 -1.2 2,093,071 + hisH b2023 0.87 -2.0 2,093,079 - hisH b2023 0.88 -4.9 2,093,079 - hisH b2023 0.88 -4.9 2,093,079 - hisH b2023 0.88 -2.5 2,093,079 - hisH b2023 0.88 -4.7 2,093,094 - -3.3 2,093,183 + -6.0 2,093,183 + -3.8 2,093,454 - hisA b2024 0.41 -4.7 2,093,510 - hisA b2024 0.49 -6.0
Or see this region's nucleotide sequence