Experiment: Ying_sugar7 rep A; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpD and trpE overlap by 1 nucleotides trpE and trpL are separated by 91 nucleotides trpL and yciV are separated by 137 nucleotides
b1263: trpD - bifunctional indole-3-glycerol-phosphate synthase/anthranilate phosphoribosyltransferase (NCBI), at 1,317,813 to 1,319,408
trpD
b1264: trpE - anthranilate synthase component I (NCBI), at 1,319,408 to 1,320,970
trpE
b1265: trpL - trp operon leader peptide (NCBI), at 1,321,062 to 1,321,106
trpL
b1266: yciV - hypothetical protein (NCBI), at 1,321,244 to 1,322,125
yciV
Position (kb)
1319
1320
1321 Strain fitness (log2 ratio)
-2
-1
0
1 at 1319.016 kb on - strand, within trpD at 1319.138 kb on - strand, within trpD at 1319.228 kb on + strand, within trpD at 1319.372 kb on - strand at 1319.455 kb on - strand at 1319.714 kb on + strand, within trpE at 1319.820 kb on + strand, within trpE at 1320.027 kb on + strand, within trpE at 1320.044 kb on + strand, within trpE at 1320.100 kb on - strand, within trpE at 1320.119 kb on - strand, within trpE at 1320.125 kb on + strand, within trpE at 1320.125 kb on + strand, within trpE at 1320.275 kb on - strand, within trpE at 1320.275 kb on - strand, within trpE at 1320.672 kb on - strand, within trpE at 1320.809 kb on - strand, within trpE at 1320.890 kb on - strand at 1321.053 kb on - strand at 1321.053 kb on - strand at 1321.257 kb on + strand at 1321.332 kb on + strand at 1321.332 kb on + strand at 1321.378 kb on + strand, within yciV at 1321.381 kb on + strand, within yciV at 1321.537 kb on - strand, within yciV at 1321.537 kb on - strand, within yciV at 1321.697 kb on + strand, within yciV at 1321.935 kb on - strand, within yciV
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep A; time point 1 remove 1,319,016 - trpD b1263 0.75 -0.7 1,319,138 - trpD b1263 0.83 -0.6 1,319,228 + trpD b1263 0.89 -1.5 1,319,372 - -0.6 1,319,455 - +0.4 1,319,714 + trpE b1264 0.20 -0.2 1,319,820 + trpE b1264 0.26 -1.7 1,320,027 + trpE b1264 0.40 -1.9 1,320,044 + trpE b1264 0.41 -2.0 1,320,100 - trpE b1264 0.44 -0.8 1,320,119 - trpE b1264 0.45 -0.2 1,320,125 + trpE b1264 0.46 -1.4 1,320,125 + trpE b1264 0.46 -1.4 1,320,275 - trpE b1264 0.55 -0.6 1,320,275 - trpE b1264 0.55 -0.5 1,320,672 - trpE b1264 0.81 -0.6 1,320,809 - trpE b1264 0.90 +0.3 1,320,890 - +0.6 1,321,053 - -0.6 1,321,053 - +0.1 1,321,257 + -0.4 1,321,332 + -0.1 1,321,332 + -0.4 1,321,378 + yciV b1266 0.15 -0.5 1,321,381 + yciV b1266 0.16 -0.4 1,321,537 - yciV b1266 0.33 +0.4 1,321,537 - yciV b1266 0.33 -0.2 1,321,697 + yciV b1266 0.51 -0.4 1,321,935 - yciV b1266 0.78 +0.2
Or see this region's nucleotide sequence