Experiment: Ying_OrganicAcid7 rep C; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recJ and dsbC are separated by 5 nucleotides dsbC and xerD are separated by 24 nucleotides xerD and fldB are separated by 111 nucleotides fldB and ygfX are separated by 39 nucleotides
b2892: recJ - ssDNA exonuclease, 5' --> 3'-specific (NCBI), at 3,034,395 to 3,036,128
recJ
b2893: dsbC - protein disulfide isomerase II (NCBI), at 3,036,134 to 3,036,844
dsbC
b2894: xerD - site-specific tyrosine recombinase XerD (NCBI), at 3,036,869 to 3,037,765
xerD
b2895: fldB - flavodoxin 2 (NCBI), at 3,037,877 to 3,038,398
fldB
b2896: ygfX - hypothetical protein (NCBI), at 3,038,438 to 3,038,845
ygfX
Position (kb)
3036
3037
3038 Strain fitness (log2 ratio)
-1
0
1 at 3035.869 kb on - strand, within recJ at 3035.906 kb on - strand, within recJ at 3035.906 kb on - strand at 3035.960 kb on - strand at 3035.996 kb on - strand at 3035.996 kb on - strand at 3035.996 kb on - strand at 3036.046 kb on - strand at 3036.068 kb on - strand at 3036.229 kb on + strand, within dsbC at 3036.229 kb on - strand, within dsbC at 3036.268 kb on + strand, within dsbC at 3036.457 kb on - strand, within dsbC at 3036.511 kb on + strand, within dsbC at 3036.511 kb on + strand, within dsbC at 3036.613 kb on - strand, within dsbC at 3036.626 kb on - strand, within dsbC at 3036.660 kb on - strand, within dsbC at 3036.660 kb on - strand, within dsbC at 3036.666 kb on + strand, within dsbC at 3036.668 kb on + strand, within dsbC at 3036.761 kb on + strand, within dsbC at 3037.372 kb on + strand, within xerD at 3037.773 kb on + strand at 3037.777 kb on + strand at 3037.777 kb on + strand at 3037.900 kb on + strand at 3037.900 kb on + strand at 3037.986 kb on - strand, within fldB at 3038.097 kb on + strand, within fldB at 3038.128 kb on + strand, within fldB at 3038.128 kb on + strand, within fldB at 3038.128 kb on + strand, within fldB at 3038.149 kb on + strand, within fldB at 3038.149 kb on + strand, within fldB at 3038.321 kb on + strand, within fldB at 3038.348 kb on - strand at 3038.348 kb on - strand at 3038.418 kb on - strand at 3038.516 kb on - strand, within ygfX at 3038.698 kb on - strand, within ygfX at 3038.714 kb on + strand, within ygfX at 3038.714 kb on + strand, within ygfX at 3038.716 kb on - strand, within ygfX at 3038.718 kb on + strand, within ygfX at 3038.718 kb on + strand, within ygfX at 3038.746 kb on - strand, within ygfX
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep C; time point 1 remove 3,035,869 - recJ b2892 0.85 -0.3 3,035,906 - recJ b2892 0.87 -0.4 3,035,906 - -0.7 3,035,960 - +0.4 3,035,996 - -0.1 3,035,996 - -0.6 3,035,996 - +0.3 3,036,046 - +0.8 3,036,068 - -0.1 3,036,229 + dsbC b2893 0.13 -0.3 3,036,229 - dsbC b2893 0.13 -0.7 3,036,268 + dsbC b2893 0.19 +0.3 3,036,457 - dsbC b2893 0.45 -0.5 3,036,511 + dsbC b2893 0.53 +0.3 3,036,511 + dsbC b2893 0.53 -0.5 3,036,613 - dsbC b2893 0.67 +0.4 3,036,626 - dsbC b2893 0.69 -0.4 3,036,660 - dsbC b2893 0.74 -1.7 3,036,660 - dsbC b2893 0.74 -0.6 3,036,666 + dsbC b2893 0.75 -0.4 3,036,668 + dsbC b2893 0.75 -0.1 3,036,761 + dsbC b2893 0.88 -0.1 3,037,372 + xerD b2894 0.56 +0.1 3,037,773 + -0.3 3,037,777 + +0.4 3,037,777 + +0.5 3,037,900 + -0.0 3,037,900 + -0.1 3,037,986 - fldB b2895 0.21 -0.3 3,038,097 + fldB b2895 0.42 +0.1 3,038,128 + fldB b2895 0.48 +0.3 3,038,128 + fldB b2895 0.48 +0.3 3,038,128 + fldB b2895 0.48 +0.7 3,038,149 + fldB b2895 0.52 -0.4 3,038,149 + fldB b2895 0.52 -0.8 3,038,321 + fldB b2895 0.85 -0.9 3,038,348 - +0.5 3,038,348 - -0.8 3,038,418 - +0.2 3,038,516 - ygfX b2896 0.19 +0.0 3,038,698 - ygfX b2896 0.64 +0.0 3,038,714 + ygfX b2896 0.68 +0.4 3,038,714 + ygfX b2896 0.68 +1.0 3,038,716 - ygfX b2896 0.68 -0.2 3,038,718 + ygfX b2896 0.69 -0.0 3,038,718 + ygfX b2896 0.69 +0.1 3,038,746 - ygfX b2896 0.75 -0.0
Or see this region's nucleotide sequence