Strain Fitness in Escherichia coli BW25113 around b2474

Experiment: Ying_OrganicAcid7 rep C; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntypfN and ypfH are separated by 109 nucleotidesypfH and ypfI are separated by 73 nucleotidesypfI and ypfJ are separated by 14 nucleotides b4547: ypfN - hypothetical protein (NCBI), at 2,590,784 to 2,590,984 ypfN b2473: ypfH - orf, hypothetical protein (VIMSS), at 2,591,094 to 2,591,792 ypfH b2474: ypfI - predicted hydrolase (NCBI), at 2,591,866 to 2,593,881 ypfI b2475: ypfJ - hypothetical protein (NCBI), at 2,593,896 to 2,594,759 ypfJ Position (kb) 2591 2592 2593 2594Strain fitness (log2 ratio) -1 0 1at 2590.934 kb on + strand, within ypfNat 2590.934 kb on + strand, within ypfNat 2590.954 kb on - strand, within ypfNat 2590.954 kb on - strand, within ypfNat 2590.997 kb on + strandat 2591.119 kb on - strandat 2591.119 kb on - strandat 2591.127 kb on - strandat 2591.178 kb on - strand, within ypfHat 2591.178 kb on - strand, within ypfHat 2591.358 kb on - strand, within ypfHat 2591.372 kb on + strand, within ypfHat 2591.372 kb on + strand, within ypfHat 2591.380 kb on - strand, within ypfHat 2591.489 kb on + strand, within ypfHat 2591.494 kb on + strand, within ypfHat 2591.584 kb on - strand, within ypfHat 2591.584 kb on - strand, within ypfHat 2591.680 kb on - strand, within ypfHat 2591.720 kb on - strand, within ypfHat 2591.720 kb on - strand, within ypfHat 2591.752 kb on + strandat 2591.912 kb on - strandat 2591.978 kb on - strandat 2591.978 kb on - strandat 2591.999 kb on - strandat 2592.010 kb on - strandat 2592.105 kb on - strand, within ypfIat 2592.105 kb on - strand, within ypfIat 2592.141 kb on + strand, within ypfIat 2592.292 kb on - strand, within ypfIat 2592.292 kb on - strand, within ypfIat 2592.385 kb on + strand, within ypfIat 2592.385 kb on + strand, within ypfIat 2592.426 kb on + strand, within ypfIat 2592.448 kb on + strand, within ypfIat 2592.471 kb on - strand, within ypfIat 2592.531 kb on - strand, within ypfIat 2592.531 kb on - strand, within ypfIat 2592.535 kb on - strand, within ypfIat 2592.557 kb on + strand, within ypfIat 2592.576 kb on - strand, within ypfIat 2592.576 kb on - strand, within ypfIat 2592.581 kb on - strand, within ypfIat 2592.581 kb on - strand, within ypfIat 2592.751 kb on - strand, within ypfIat 2592.784 kb on - strand, within ypfIat 2592.942 kb on + strand, within ypfIat 2592.942 kb on + strand, within ypfIat 2592.942 kb on + strand, within ypfIat 2593.020 kb on - strand, within ypfIat 2593.303 kb on + strand, within ypfIat 2593.380 kb on - strand, within ypfIat 2593.380 kb on - strand, within ypfIat 2593.380 kb on - strand, within ypfIat 2593.394 kb on - strand, within ypfIat 2593.424 kb on - strand, within ypfIat 2593.424 kb on - strand, within ypfIat 2593.509 kb on - strand, within ypfIat 2593.509 kb on - strand, within ypfIat 2593.767 kb on + strandat 2593.775 kb on - strandat 2593.775 kb on - strandat 2593.960 kb on - strandat 2593.973 kb on + strandat 2594.113 kb on - strand, within ypfJat 2594.113 kb on - strand, within ypfJat 2594.224 kb on + strand, within ypfJat 2594.224 kb on + strand, within ypfJat 2594.282 kb on - strand, within ypfJat 2594.282 kb on - strand, within ypfJat 2594.331 kb on - strand, within ypfJat 2594.338 kb on + strand, within ypfJat 2594.375 kb on + strand, within ypfJat 2594.385 kb on - strand, within ypfJat 2594.430 kb on - strand, within ypfJat 2594.449 kb on + strand, within ypfJat 2594.449 kb on + strand, within ypfJat 2594.449 kb on + strand, within ypfJat 2594.449 kb on - strand, within ypfJat 2594.540 kb on + strand, within ypfJat 2594.573 kb on + strand, within ypfJat 2594.608 kb on - strand, within ypfJat 2594.720 kb on + strandat 2594.872 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep C; time point 1
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2,590,934 + ypfN b4547 0.75 -0.0
2,590,934 + ypfN b4547 0.75 -0.2
2,590,954 - ypfN b4547 0.85 -0.2
2,590,954 - ypfN b4547 0.85 -0.3
2,590,997 + +0.3
2,591,119 - +0.4
2,591,119 - +0.4
2,591,127 - +0.2
2,591,178 - ypfH b2473 0.12 -0.2
2,591,178 - ypfH b2473 0.12 -0.0
2,591,358 - ypfH b2473 0.38 -0.6
2,591,372 + ypfH b2473 0.40 +0.1
2,591,372 + ypfH b2473 0.40 +0.4
2,591,380 - ypfH b2473 0.41 -0.2
2,591,489 + ypfH b2473 0.57 +0.9
2,591,494 + ypfH b2473 0.57 -0.1
2,591,584 - ypfH b2473 0.70 +0.8
2,591,584 - ypfH b2473 0.70 -0.8
2,591,680 - ypfH b2473 0.84 +0.0
2,591,720 - ypfH b2473 0.90 +0.1
2,591,720 - ypfH b2473 0.90 +0.2
2,591,752 + -0.2
2,591,912 - -0.2
2,591,978 - +0.7
2,591,978 - +0.4
2,591,999 - -0.3
2,592,010 - +0.3
2,592,105 - ypfI b2474 0.12 +0.4
2,592,105 - ypfI b2474 0.12 +0.6
2,592,141 + ypfI b2474 0.14 +0.4
2,592,292 - ypfI b2474 0.21 -0.6
2,592,292 - ypfI b2474 0.21 -0.6
2,592,385 + ypfI b2474 0.26 +0.0
2,592,385 + ypfI b2474 0.26 -0.6
2,592,426 + ypfI b2474 0.28 +1.5
2,592,448 + ypfI b2474 0.29 +0.4
2,592,471 - ypfI b2474 0.30 +0.5
2,592,531 - ypfI b2474 0.33 +0.3
2,592,531 - ypfI b2474 0.33 -0.2
2,592,535 - ypfI b2474 0.33 +0.7
2,592,557 + ypfI b2474 0.34 +0.7
2,592,576 - ypfI b2474 0.35 +0.7
2,592,576 - ypfI b2474 0.35 -0.7
2,592,581 - ypfI b2474 0.35 +0.5
2,592,581 - ypfI b2474 0.35 +0.4
2,592,751 - ypfI b2474 0.44 +0.5
2,592,784 - ypfI b2474 0.46 +1.0
2,592,942 + ypfI b2474 0.53 +0.1
2,592,942 + ypfI b2474 0.53 +1.5
2,592,942 + ypfI b2474 0.53 +0.5
2,593,020 - ypfI b2474 0.57 +0.3
2,593,303 + ypfI b2474 0.71 -0.1
2,593,380 - ypfI b2474 0.75 +0.6
2,593,380 - ypfI b2474 0.75 -0.1
2,593,380 - ypfI b2474 0.75 +0.8
2,593,394 - ypfI b2474 0.76 +0.5
2,593,424 - ypfI b2474 0.77 +0.1
2,593,424 - ypfI b2474 0.77 +0.6
2,593,509 - ypfI b2474 0.81 +0.5
2,593,509 - ypfI b2474 0.81 +0.4
2,593,767 + -0.1
2,593,775 - +0.4
2,593,775 - +0.2
2,593,960 - -0.3
2,593,973 + +0.2
2,594,113 - ypfJ b2475 0.25 +0.1
2,594,113 - ypfJ b2475 0.25 -0.6
2,594,224 + ypfJ b2475 0.38 -0.4
2,594,224 + ypfJ b2475 0.38 +0.8
2,594,282 - ypfJ b2475 0.45 -0.1
2,594,282 - ypfJ b2475 0.45 -0.2
2,594,331 - ypfJ b2475 0.50 -0.1
2,594,338 + ypfJ b2475 0.51 +0.1
2,594,375 + ypfJ b2475 0.55 +1.0
2,594,385 - ypfJ b2475 0.57 -0.4
2,594,430 - ypfJ b2475 0.62 +0.0
2,594,449 + ypfJ b2475 0.64 -0.3
2,594,449 + ypfJ b2475 0.64 -0.0
2,594,449 + ypfJ b2475 0.64 +1.2
2,594,449 - ypfJ b2475 0.64 -0.6
2,594,540 + ypfJ b2475 0.75 +0.3
2,594,573 + ypfJ b2475 0.78 -0.4
2,594,608 - ypfJ b2475 0.82 -0.2
2,594,720 + -0.3
2,594,872 - +0.3

Or see this region's nucleotide sequence