Experiment: Ying_OrganicAcid7 rep C; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purF and cvpA are separated by 36 nucleotides cvpA and dedD are separated by 258 nucleotides dedD and folC overlap by 11 nucleotides
b2312: purF - amidophosphoribosyltransferase (NCBI), at 2,426,743 to 2,428,260
purF
b2313: cvpA - membrane protein required for colicin V production (NCBI), at 2,428,297 to 2,428,785
cvpA
b2314: dedD - putative lipoprotein (VIMSS), at 2,429,044 to 2,429,706
dedD
b2315: folC - bifunctional folylpolyglutamate synthase/ dihydrofolate synthase (NCBI), at 2,429,696 to 2,430,964
folC
Position (kb)
2428
2429 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2427.297 kb on - strand, within purF at 2427.537 kb on + strand, within purF at 2427.605 kb on - strand, within purF at 2427.649 kb on - strand, within purF at 2427.754 kb on + strand, within purF at 2427.948 kb on + strand, within purF at 2427.948 kb on + strand, within purF at 2427.966 kb on + strand, within purF at 2427.974 kb on - strand, within purF at 2428.011 kb on - strand, within purF at 2428.042 kb on - strand, within purF at 2428.063 kb on + strand, within purF at 2428.064 kb on - strand, within purF at 2428.064 kb on - strand, within purF at 2428.146 kb on - strand at 2428.157 kb on - strand at 2428.194 kb on + strand at 2428.194 kb on + strand at 2428.202 kb on - strand at 2428.202 kb on - strand at 2428.203 kb on - strand at 2428.385 kb on - strand, within cvpA at 2428.458 kb on - strand, within cvpA at 2428.510 kb on - strand, within cvpA at 2428.649 kb on + strand, within cvpA at 2428.657 kb on - strand, within cvpA at 2428.657 kb on - strand, within cvpA at 2428.684 kb on - strand, within cvpA at 2428.723 kb on + strand, within cvpA at 2428.775 kb on + strand at 2428.775 kb on + strand at 2428.816 kb on - strand at 2428.818 kb on - strand at 2428.818 kb on - strand at 2428.986 kb on - strand at 2429.015 kb on - strand at 2429.016 kb on + strand at 2429.072 kb on - strand at 2429.111 kb on - strand, within dedD at 2429.281 kb on - strand, within dedD at 2429.281 kb on - strand, within dedD at 2429.287 kb on - strand, within dedD at 2429.461 kb on - strand, within dedD at 2429.480 kb on + strand, within dedD at 2429.596 kb on - strand, within dedD at 2429.596 kb on - strand, within dedD at 2429.646 kb on - strand at 2429.646 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep C; time point 1 remove 2,427,297 - purF b2312 0.36 -3.1 2,427,537 + purF b2312 0.52 -0.6 2,427,605 - purF b2312 0.57 -2.4 2,427,649 - purF b2312 0.60 -2.8 2,427,754 + purF b2312 0.67 -2.5 2,427,948 + purF b2312 0.79 -2.2 2,427,948 + purF b2312 0.79 -2.8 2,427,966 + purF b2312 0.81 -2.8 2,427,974 - purF b2312 0.81 -0.1 2,428,011 - purF b2312 0.84 -2.1 2,428,042 - purF b2312 0.86 -2.2 2,428,063 + purF b2312 0.87 -2.0 2,428,064 - purF b2312 0.87 -1.8 2,428,064 - purF b2312 0.87 -2.0 2,428,146 - -2.7 2,428,157 - -1.8 2,428,194 + -3.2 2,428,194 + -0.3 2,428,202 - -1.0 2,428,202 - -2.0 2,428,203 - -1.7 2,428,385 - cvpA b2313 0.18 -2.0 2,428,458 - cvpA b2313 0.33 -2.4 2,428,510 - cvpA b2313 0.44 -1.5 2,428,649 + cvpA b2313 0.72 -1.6 2,428,657 - cvpA b2313 0.74 +0.7 2,428,657 - cvpA b2313 0.74 +0.2 2,428,684 - cvpA b2313 0.79 -0.1 2,428,723 + cvpA b2313 0.87 -0.7 2,428,775 + -0.7 2,428,775 + -0.6 2,428,816 - +0.2 2,428,818 - -0.8 2,428,818 - +1.5 2,428,986 - -0.1 2,429,015 - +0.4 2,429,016 + -0.1 2,429,072 - -0.5 2,429,111 - dedD b2314 0.10 +0.2 2,429,281 - dedD b2314 0.36 -1.1 2,429,281 - dedD b2314 0.36 +0.6 2,429,287 - dedD b2314 0.37 +0.5 2,429,461 - dedD b2314 0.63 +0.6 2,429,480 + dedD b2314 0.66 +0.6 2,429,596 - dedD b2314 0.83 +1.0 2,429,596 - dedD b2314 0.83 -0.6 2,429,646 - +0.7 2,429,646 - +0.3
Or see this region's nucleotide sequence