Experiment: Ying_OrganicAcid7 rep C; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisD and hisC overlap by 4 nucleotides hisC and hisB overlap by 1 nucleotides
b2020: hisD - histidinol dehydrogenase (NCBI), at 2,089,121 to 2,090,425
hisD
b2021: hisC - histidinol-phosphate aminotransferase (NCBI), at 2,090,422 to 2,091,492
hisC
b2022: hisB - imidazoleglycerolphosphate dehydratase and histidinol-phosphate phosphatase (VIMSS), at 2,091,492 to 2,092,559
hisB
Position (kb)
2090
2091
2092 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2089.504 kb on + strand, within hisD at 2089.605 kb on - strand, within hisD at 2089.755 kb on - strand, within hisD at 2089.776 kb on + strand, within hisD at 2089.844 kb on + strand, within hisD at 2089.929 kb on - strand, within hisD at 2089.929 kb on - strand, within hisD at 2090.018 kb on - strand, within hisD at 2090.018 kb on - strand, within hisD at 2090.075 kb on - strand, within hisD at 2090.124 kb on + strand, within hisD at 2090.124 kb on + strand, within hisD at 2090.132 kb on - strand, within hisD at 2090.300 kb on - strand at 2090.337 kb on - strand at 2090.419 kb on + strand at 2090.721 kb on - strand, within hisC at 2090.808 kb on - strand, within hisC at 2090.932 kb on + strand, within hisC at 2090.932 kb on + strand, within hisC at 2090.938 kb on + strand, within hisC at 2091.102 kb on + strand, within hisC at 2091.202 kb on - strand, within hisC at 2091.202 kb on - strand, within hisC at 2091.274 kb on + strand, within hisC at 2091.351 kb on + strand, within hisC at 2091.593 kb on - strand at 2091.593 kb on - strand at 2091.619 kb on - strand, within hisB at 2091.659 kb on + strand, within hisB at 2091.985 kb on - strand, within hisB at 2092.013 kb on - strand, within hisB at 2092.013 kb on - strand, within hisB at 2092.070 kb on + strand, within hisB at 2092.080 kb on + strand, within hisB at 2092.114 kb on - strand, within hisB at 2092.160 kb on + strand, within hisB at 2092.171 kb on - strand, within hisB at 2092.193 kb on - strand, within hisB at 2092.193 kb on - strand, within hisB at 2092.355 kb on + strand, within hisB at 2092.355 kb on + strand, within hisB at 2092.359 kb on - strand, within hisB at 2092.406 kb on + strand, within hisB at 2092.406 kb on + strand, within hisB at 2092.412 kb on + strand, within hisB at 2092.412 kb on + strand, within hisB at 2092.464 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep C; time point 1 remove 2,089,504 + hisD b2020 0.29 -2.9 2,089,605 - hisD b2020 0.37 -2.6 2,089,755 - hisD b2020 0.49 -2.8 2,089,776 + hisD b2020 0.50 -2.4 2,089,844 + hisD b2020 0.55 -2.5 2,089,929 - hisD b2020 0.62 -3.2 2,089,929 - hisD b2020 0.62 -2.4 2,090,018 - hisD b2020 0.69 -1.1 2,090,018 - hisD b2020 0.69 -2.5 2,090,075 - hisD b2020 0.73 -1.6 2,090,124 + hisD b2020 0.77 -3.0 2,090,124 + hisD b2020 0.77 -3.1 2,090,132 - hisD b2020 0.77 -2.4 2,090,300 - -2.2 2,090,337 - -1.9 2,090,419 + -0.0 2,090,721 - hisC b2021 0.28 -1.3 2,090,808 - hisC b2021 0.36 -3.6 2,090,932 + hisC b2021 0.48 -2.2 2,090,932 + hisC b2021 0.48 -2.0 2,090,938 + hisC b2021 0.48 -3.7 2,091,102 + hisC b2021 0.63 -2.5 2,091,202 - hisC b2021 0.73 -1.1 2,091,202 - hisC b2021 0.73 -1.8 2,091,274 + hisC b2021 0.80 -3.8 2,091,351 + hisC b2021 0.87 -2.7 2,091,593 - -0.4 2,091,593 - -0.4 2,091,619 - hisB b2022 0.12 -1.7 2,091,659 + hisB b2022 0.16 -2.2 2,091,985 - hisB b2022 0.46 -1.6 2,092,013 - hisB b2022 0.49 -2.2 2,092,013 - hisB b2022 0.49 -2.4 2,092,070 + hisB b2022 0.54 -3.2 2,092,080 + hisB b2022 0.55 -2.2 2,092,114 - hisB b2022 0.58 -1.7 2,092,160 + hisB b2022 0.63 -1.7 2,092,171 - hisB b2022 0.64 -1.3 2,092,193 - hisB b2022 0.66 -2.2 2,092,193 - hisB b2022 0.66 -0.6 2,092,355 + hisB b2022 0.81 -2.3 2,092,355 + hisB b2022 0.81 -2.2 2,092,359 - hisB b2022 0.81 -1.9 2,092,406 + hisB b2022 0.86 -0.3 2,092,406 + hisB b2022 0.86 -2.7 2,092,412 + hisB b2022 0.86 -2.3 2,092,412 + hisB b2022 0.86 -2.4 2,092,464 + -2.4
Or see this region's nucleotide sequence