Experiment: Ying_OrganicAcid7 rep C; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yoeB and yefM overlap by 4 nucleotides yefM and hisL are separated by 282 nucleotides hisL and hisG are separated by 145 nucleotides hisG and hisD are separated by 5 nucleotides
b4539: yoeB - toxin of the YoeB-YefM toxin-antitoxin system (NCBI), at 2,087,235 to 2,087,489
yoeB
b2017: yefM - orf, hypothetical protein (VIMSS), at 2,087,486 to 2,087,737
yefM
b2018: hisL - his operon leader peptide (NCBI), at 2,088,020 to 2,088,070
hisL
b2019: hisG - ATP phosphoribosyltransferase (NCBI), at 2,088,216 to 2,089,115
hisG
b2020: hisD - histidinol dehydrogenase (NCBI), at 2,089,121 to 2,090,425
hisD
Position (kb)
2088
2089
2090 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2087.364 kb on - strand, within yoeB at 2087.506 kb on - strand at 2087.506 kb on - strand at 2087.544 kb on + strand, within yefM at 2087.642 kb on - strand, within yefM at 2087.867 kb on + strand at 2088.113 kb on - strand at 2088.203 kb on - strand at 2088.203 kb on - strand at 2088.316 kb on + strand, within hisG at 2088.484 kb on - strand, within hisG at 2088.602 kb on + strand, within hisG at 2088.610 kb on - strand, within hisG at 2088.610 kb on - strand, within hisG at 2088.657 kb on - strand, within hisG at 2088.681 kb on - strand, within hisG at 2088.859 kb on + strand, within hisG at 2088.900 kb on + strand, within hisG at 2088.900 kb on + strand, within hisG at 2089.029 kb on + strand at 2089.104 kb on + strand at 2089.133 kb on - strand at 2089.155 kb on - strand at 2089.187 kb on - strand at 2089.234 kb on - strand at 2089.234 kb on - strand at 2089.267 kb on + strand, within hisD at 2089.326 kb on + strand, within hisD at 2089.419 kb on - strand, within hisD at 2089.504 kb on + strand, within hisD at 2089.605 kb on - strand, within hisD at 2089.755 kb on - strand, within hisD at 2089.776 kb on + strand, within hisD at 2089.844 kb on + strand, within hisD at 2089.929 kb on - strand, within hisD at 2089.929 kb on - strand, within hisD at 2090.018 kb on - strand, within hisD at 2090.018 kb on - strand, within hisD at 2090.075 kb on - strand, within hisD
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep C; time point 1 remove 2,087,364 - yoeB b4539 0.51 +0.2 2,087,506 - -0.3 2,087,506 - +0.5 2,087,544 + yefM b2017 0.23 -0.0 2,087,642 - yefM b2017 0.62 +0.6 2,087,867 + +0.2 2,088,113 - -0.7 2,088,203 - -1.4 2,088,203 - -1.2 2,088,316 + hisG b2019 0.11 -1.7 2,088,484 - hisG b2019 0.30 -2.7 2,088,602 + hisG b2019 0.43 -2.2 2,088,610 - hisG b2019 0.44 -2.7 2,088,610 - hisG b2019 0.44 -1.8 2,088,657 - hisG b2019 0.49 -3.2 2,088,681 - hisG b2019 0.52 -1.9 2,088,859 + hisG b2019 0.71 -0.5 2,088,900 + hisG b2019 0.76 -1.0 2,088,900 + hisG b2019 0.76 -2.4 2,089,029 + -2.0 2,089,104 + -2.7 2,089,133 - -2.1 2,089,155 - -2.6 2,089,187 - -2.2 2,089,234 - -1.5 2,089,234 - -3.0 2,089,267 + hisD b2020 0.11 -1.6 2,089,326 + hisD b2020 0.16 -1.7 2,089,419 - hisD b2020 0.23 -2.7 2,089,504 + hisD b2020 0.29 -2.9 2,089,605 - hisD b2020 0.37 -2.6 2,089,755 - hisD b2020 0.49 -2.8 2,089,776 + hisD b2020 0.50 -2.4 2,089,844 + hisD b2020 0.55 -2.5 2,089,929 - hisD b2020 0.62 -3.2 2,089,929 - hisD b2020 0.62 -2.4 2,090,018 - hisD b2020 0.69 -1.1 2,090,018 - hisD b2020 0.69 -2.5 2,090,075 - hisD b2020 0.73 -1.6
Or see this region's nucleotide sequence