Experiment: Ying_OrganicAcid7 rep C; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nhaB and fadR are separated by 220 nucleotides fadR and ycgB are separated by 51 nucleotides ycgB and dadA are separated by 329 nucleotides
b1186: nhaB - NhaB sodium/proton transporter (Katherine Huang), at 1,232,399 to 1,233,940
nhaB
b1187: fadR - fatty acid metabolism regulator (NCBI), at 1,234,161 to 1,234,880
fadR
b1188: ycgB - ycgB component of nitrogen-related signalling system (of yeaGH-ycgB) (from data) , at 1,234,932 to 1,236,464
ycgB
b1189: dadA - D-amino acid dehydrogenase (quinone) DadA (EC 1.4.5.1) (from data) , at 1,236,794 to 1,238,092
dadA
Position (kb)
1234
1235
1236
1237 Strain fitness (log2 ratio)
-2
-1
0
1 at 1234.004 kb on - strand at 1234.099 kb on - strand at 1234.102 kb on + strand at 1234.112 kb on + strand at 1234.114 kb on + strand at 1234.118 kb on - strand at 1234.118 kb on - strand at 1234.157 kb on + strand at 1234.222 kb on + strand at 1234.299 kb on + strand, within fadR at 1234.317 kb on - strand, within fadR at 1234.331 kb on + strand, within fadR at 1234.436 kb on - strand at 1234.565 kb on + strand, within fadR at 1234.673 kb on + strand, within fadR at 1234.681 kb on - strand, within fadR at 1234.720 kb on + strand, within fadR at 1234.807 kb on + strand, within fadR at 1234.807 kb on + strand, within fadR at 1234.815 kb on - strand at 1234.889 kb on - strand at 1234.989 kb on + strand at 1235.038 kb on - strand at 1235.069 kb on + strand at 1235.092 kb on - strand, within ycgB at 1235.092 kb on - strand, within ycgB at 1235.136 kb on - strand, within ycgB at 1235.251 kb on + strand, within ycgB at 1235.263 kb on + strand, within ycgB at 1235.263 kb on + strand, within ycgB at 1235.362 kb on - strand, within ycgB at 1235.483 kb on - strand, within ycgB at 1235.815 kb on - strand, within ycgB at 1235.815 kb on - strand, within ycgB at 1236.122 kb on + strand, within ycgB at 1236.187 kb on - strand, within ycgB at 1236.339 kb on - strand at 1236.345 kb on - strand at 1236.374 kb on + strand at 1236.374 kb on + strand at 1236.414 kb on - strand at 1236.456 kb on - strand at 1236.788 kb on - strand at 1236.788 kb on - strand at 1236.803 kb on + strand at 1236.924 kb on - strand, within dadA at 1237.037 kb on + strand, within dadA at 1237.040 kb on - strand, within dadA at 1237.146 kb on + strand, within dadA at 1237.180 kb on + strand, within dadA at 1237.213 kb on + strand, within dadA at 1237.213 kb on + strand, within dadA at 1237.218 kb on + strand, within dadA
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep C; time point 1 remove 1,234,004 - +0.1 1,234,099 - +0.3 1,234,102 + +0.6 1,234,112 + -0.2 1,234,114 + -0.6 1,234,118 - -0.2 1,234,118 - -0.5 1,234,157 + -0.8 1,234,222 + +0.3 1,234,299 + fadR b1187 0.19 +0.2 1,234,317 - fadR b1187 0.22 +0.2 1,234,331 + fadR b1187 0.24 +0.8 1,234,436 - +0.1 1,234,565 + fadR b1187 0.56 +0.7 1,234,673 + fadR b1187 0.71 +1.0 1,234,681 - fadR b1187 0.72 +0.4 1,234,720 + fadR b1187 0.78 +0.5 1,234,807 + fadR b1187 0.90 +0.2 1,234,807 + fadR b1187 0.90 +0.6 1,234,815 - +1.6 1,234,889 - +0.0 1,234,989 + -0.7 1,235,038 - -1.3 1,235,069 + -0.7 1,235,092 - ycgB b1188 0.10 -1.0 1,235,092 - ycgB b1188 0.10 -1.1 1,235,136 - ycgB b1188 0.13 -1.8 1,235,251 + ycgB b1188 0.21 -1.2 1,235,263 + ycgB b1188 0.22 -0.9 1,235,263 + ycgB b1188 0.22 -2.1 1,235,362 - ycgB b1188 0.28 -0.7 1,235,483 - ycgB b1188 0.36 -1.4 1,235,815 - ycgB b1188 0.58 -1.3 1,235,815 - ycgB b1188 0.58 -1.1 1,236,122 + ycgB b1188 0.78 -1.0 1,236,187 - ycgB b1188 0.82 -0.8 1,236,339 - -1.3 1,236,345 - -2.2 1,236,374 + -1.5 1,236,374 + -0.4 1,236,414 - -0.5 1,236,456 - -0.6 1,236,788 - +0.3 1,236,788 - +0.4 1,236,803 + -0.1 1,236,924 - dadA b1189 0.10 +0.4 1,237,037 + dadA b1189 0.19 +0.5 1,237,040 - dadA b1189 0.19 -0.3 1,237,146 + dadA b1189 0.27 +0.3 1,237,180 + dadA b1189 0.30 +0.5 1,237,213 + dadA b1189 0.32 +0.4 1,237,213 + dadA b1189 0.32 +1.1 1,237,218 + dadA b1189 0.33 +0.0
Or see this region's nucleotide sequence