Experiment: Ying_OrganicAcid7 rep C; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tolQ and tolR are separated by 3 nucleotides tolR and tolA are separated by 64 nucleotides tolA and tolB are separated by 132 nucleotides
b0737: tolQ - membrane spanning protein in TolA-TolQ-TolR complex (NCBI), at 774,376 to 775,068
tolQ
b0738: tolR - membrane spanning protein in TolA-TolQ-TolR complex (NCBI), at 775,072 to 775,500
tolR
b0739: tolA - cell envelope integrity inner membrane protein TolA (NCBI), at 775,565 to 776,830
tolA
b0740: tolB - translocation protein TolB precursor (NCBI), at 776,963 to 778,255
tolB
Position (kb) 775
776
777 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 774.571 kb on + strand, within tolQ at 774.649 kb on + strand, within tolQ at 774.668 kb on - strand, within tolQ at 774.723 kb on + strand, within tolQ at 774.723 kb on + strand, within tolQ at 774.809 kb on - strand, within tolQ at 774.813 kb on - strand, within tolQ at 774.818 kb on - strand, within tolQ at 774.839 kb on + strand, within tolQ at 774.905 kb on + strand, within tolQ at 775.063 kb on + strand at 775.063 kb on + strand at 775.070 kb on - strand at 775.071 kb on - strand at 775.071 kb on - strand at 775.091 kb on - strand at 775.121 kb on + strand, within tolR at 775.121 kb on + strand, within tolR at 775.121 kb on + strand, within tolR at 775.146 kb on + strand, within tolR at 775.152 kb on - strand, within tolR at 775.194 kb on - strand, within tolR at 775.258 kb on + strand, within tolR at 775.265 kb on + strand, within tolR at 775.275 kb on + strand, within tolR at 775.280 kb on + strand, within tolR at 775.288 kb on - strand, within tolR at 775.288 kb on - strand, within tolR at 775.298 kb on - strand, within tolR at 775.333 kb on + strand, within tolR at 775.333 kb on + strand, within tolR at 775.357 kb on - strand, within tolR at 775.526 kb on - strand at 775.533 kb on + strand at 775.565 kb on + strand at 775.628 kb on + strand at 775.651 kb on + strand at 775.659 kb on - strand at 775.672 kb on + strand at 775.748 kb on + strand, within tolA at 775.753 kb on + strand, within tolA at 775.840 kb on + strand, within tolA at 775.930 kb on - strand, within tolA at 775.932 kb on + strand, within tolA at 775.947 kb on + strand, within tolA at 776.098 kb on - strand, within tolA at 776.124 kb on + strand, within tolA at 776.146 kb on + strand, within tolA at 776.265 kb on - strand, within tolA at 776.265 kb on - strand, within tolA at 776.464 kb on + strand, within tolA at 776.475 kb on - strand, within tolA at 776.601 kb on - strand, within tolA at 776.625 kb on - strand, within tolA at 776.640 kb on - strand, within tolA at 776.640 kb on - strand, within tolA at 776.657 kb on - strand, within tolA at 776.713 kb on - strand at 776.725 kb on + strand at 776.779 kb on - strand at 776.943 kb on + strand at 776.968 kb on - strand at 776.994 kb on + strand at 776.994 kb on + strand at 777.012 kb on + strand at 777.014 kb on + strand at 777.135 kb on + strand, within tolB at 777.146 kb on + strand, within tolB at 777.260 kb on + strand, within tolB at 777.278 kb on - strand, within tolB at 777.299 kb on - strand, within tolB at 777.303 kb on - strand, within tolB at 777.393 kb on - strand, within tolB at 777.398 kb on - strand, within tolB at 777.444 kb on - strand, within tolB at 777.513 kb on + strand, within tolB at 777.522 kb on - strand, within tolB at 777.602 kb on - strand, within tolB at 777.602 kb on - strand, within tolB at 777.692 kb on + strand, within tolB at 777.700 kb on - strand, within tolB at 777.720 kb on - strand, within tolB at 777.770 kb on - strand, within tolB at 777.792 kb on - strand, within tolB
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep C; time point 1 remove 774,571 + tolQ b0737 0.28 -1.1 774,649 + tolQ b0737 0.39 -2.9 774,668 - tolQ b0737 0.42 -1.6 774,723 + tolQ b0737 0.50 -0.5 774,723 + tolQ b0737 0.50 -0.6 774,809 - tolQ b0737 0.62 -2.3 774,813 - tolQ b0737 0.63 -0.2 774,818 - tolQ b0737 0.64 -0.2 774,839 + tolQ b0737 0.67 -0.2 774,905 + tolQ b0737 0.76 -1.2 775,063 + -0.1 775,063 + -0.6 775,070 - +0.2 775,071 - -0.3 775,071 - -0.5 775,091 - -0.9 775,121 + tolR b0738 0.11 -1.4 775,121 + tolR b0738 0.11 -0.1 775,121 + tolR b0738 0.11 -1.5 775,146 + tolR b0738 0.17 -1.6 775,152 - tolR b0738 0.19 -0.7 775,194 - tolR b0738 0.28 -1.3 775,258 + tolR b0738 0.43 -1.7 775,265 + tolR b0738 0.45 -0.1 775,275 + tolR b0738 0.47 -0.4 775,280 + tolR b0738 0.48 -1.9 775,288 - tolR b0738 0.50 -0.9 775,288 - tolR b0738 0.50 -0.3 775,298 - tolR b0738 0.53 -2.2 775,333 + tolR b0738 0.61 -2.5 775,333 + tolR b0738 0.61 -1.1 775,357 - tolR b0738 0.66 -0.7 775,526 - -0.9 775,533 + -0.1 775,565 + -0.5 775,628 + -2.2 775,651 + -2.2 775,659 - -1.1 775,672 + -1.3 775,748 + tolA b0739 0.14 -2.4 775,753 + tolA b0739 0.15 -0.9 775,840 + tolA b0739 0.22 -0.6 775,930 - tolA b0739 0.29 +0.3 775,932 + tolA b0739 0.29 -3.6 775,947 + tolA b0739 0.30 -2.0 776,098 - tolA b0739 0.42 -1.3 776,124 + tolA b0739 0.44 -1.9 776,146 + tolA b0739 0.46 -1.0 776,265 - tolA b0739 0.55 -1.7 776,265 - tolA b0739 0.55 -0.4 776,464 + tolA b0739 0.71 -2.3 776,475 - tolA b0739 0.72 -1.5 776,601 - tolA b0739 0.82 -2.2 776,625 - tolA b0739 0.84 -3.5 776,640 - tolA b0739 0.85 -0.8 776,640 - tolA b0739 0.85 -1.5 776,657 - tolA b0739 0.86 +0.1 776,713 - -1.8 776,725 + -1.3 776,779 - -2.5 776,943 + -0.2 776,968 - -0.6 776,994 + -0.1 776,994 + -0.4 777,012 + -3.2 777,014 + +0.4 777,135 + tolB b0740 0.13 -1.5 777,146 + tolB b0740 0.14 -1.6 777,260 + tolB b0740 0.23 -1.1 777,278 - tolB b0740 0.24 -1.9 777,299 - tolB b0740 0.26 -1.5 777,303 - tolB b0740 0.26 -0.9 777,393 - tolB b0740 0.33 -0.3 777,398 - tolB b0740 0.34 -0.8 777,444 - tolB b0740 0.37 -0.6 777,513 + tolB b0740 0.43 -1.3 777,522 - tolB b0740 0.43 -2.0 777,602 - tolB b0740 0.49 +0.1 777,602 - tolB b0740 0.49 -2.9 777,692 + tolB b0740 0.56 -0.4 777,700 - tolB b0740 0.57 -1.3 777,720 - tolB b0740 0.59 -0.3 777,770 - tolB b0740 0.62 -1.0 777,792 - tolB b0740 0.64 -1.5
Or see this region's nucleotide sequence