Experiment: Ying_OrganicAcid7 rep C; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sucC and sucD overlap by 1 nucleotides sucD and mngR are separated by 103 nucleotides mngR and mngA are separated by 108 nucleotides
b0728: sucC - succinyl-CoA synthetase subunit beta (NCBI), at 762,237 to 763,403
sucC
b0729: sucD - succinyl-CoA synthetase subunit alpha (NCBI), at 763,403 to 764,272
sucD
b0730: mngR - DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI), at 764,376 to 765,098
mngR
b0731: mngA - fused 2-O-a-mannosyl-D-glycerate specific PTS enzymes: IIA component/IIB component/IIC component (NCBI), at 765,207 to 767,183
mngA
Position (kb) 763
764
765 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 762.600 kb on - strand, within sucC at 762.708 kb on + strand, within sucC at 762.708 kb on + strand, within sucC at 762.713 kb on + strand, within sucC at 762.989 kb on - strand, within sucC at 762.999 kb on - strand, within sucC at 763.015 kb on + strand, within sucC at 763.070 kb on - strand, within sucC at 763.131 kb on + strand, within sucC at 763.206 kb on - strand, within sucC at 763.206 kb on - strand, within sucC at 763.206 kb on - strand, within sucC at 763.278 kb on + strand, within sucC at 763.625 kb on + strand, within sucD at 763.625 kb on + strand, within sucD at 763.712 kb on + strand, within sucD at 763.712 kb on + strand, within sucD at 763.773 kb on + strand, within sucD at 763.871 kb on - strand, within sucD at 763.901 kb on + strand, within sucD at 764.252 kb on - strand at 764.279 kb on + strand at 764.302 kb on + strand at 764.302 kb on + strand at 764.311 kb on + strand at 764.319 kb on + strand at 764.349 kb on + strand at 764.480 kb on + strand, within mngR at 764.480 kb on + strand, within mngR at 764.515 kb on + strand, within mngR at 764.515 kb on + strand, within mngR at 764.515 kb on + strand, within mngR at 764.515 kb on + strand, within mngR at 764.645 kb on + strand, within mngR at 764.668 kb on + strand, within mngR at 764.811 kb on + strand, within mngR
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep C; time point 1 remove 762,600 - sucC b0728 0.31 -1.0 762,708 + sucC b0728 0.40 +0.0 762,708 + sucC b0728 0.40 -1.3 762,713 + sucC b0728 0.41 -1.3 762,989 - sucC b0728 0.64 -0.6 762,999 - sucC b0728 0.65 -1.4 763,015 + sucC b0728 0.67 -1.1 763,070 - sucC b0728 0.71 -0.8 763,131 + sucC b0728 0.77 +0.3 763,206 - sucC b0728 0.83 -3.1 763,206 - sucC b0728 0.83 -0.8 763,206 - sucC b0728 0.83 -0.4 763,278 + sucC b0728 0.89 -0.4 763,625 + sucD b0729 0.26 -0.5 763,625 + sucD b0729 0.26 -1.0 763,712 + sucD b0729 0.36 -0.8 763,712 + sucD b0729 0.36 -2.0 763,773 + sucD b0729 0.43 -0.9 763,871 - sucD b0729 0.54 -1.6 763,901 + sucD b0729 0.57 -0.5 764,252 - -1.0 764,279 + +0.7 764,302 + -0.1 764,302 + -0.7 764,311 + +0.0 764,319 + -0.5 764,349 + +0.2 764,480 + mngR b0730 0.14 -0.7 764,480 + mngR b0730 0.14 +0.6 764,515 + mngR b0730 0.19 +0.2 764,515 + mngR b0730 0.19 +0.9 764,515 + mngR b0730 0.19 +1.2 764,515 + mngR b0730 0.19 +2.5 764,645 + mngR b0730 0.37 -0.9 764,668 + mngR b0730 0.40 -1.3 764,811 + mngR b0730 0.60 +1.9
Or see this region's nucleotide sequence