Strain Fitness in Escherichia coli BW25113 around b4172

Experiment: Ying_OrganicAcid7 rep A; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmiaA and hfq are separated by 85 nucleotideshfq and hflX are separated by 75 nucleotides b4171: miaA - tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI), at 4,397,275 to 4,398,225 miaA b4172: hfq - RNA-binding protein Hfq (NCBI), at 4,398,311 to 4,398,619 hfq b4173: hflX - predicted GTPase (NCBI), at 4,398,695 to 4,399,975 hflX Position (kb) 4398 4399Strain fitness (log2 ratio) -2 -1 0 1 2at 4397.420 kb on + strand, within miaAat 4397.449 kb on + strand, within miaAat 4397.449 kb on + strand, within miaAat 4397.455 kb on + strand, within miaAat 4397.458 kb on - strand, within miaAat 4397.505 kb on + strand, within miaAat 4397.549 kb on - strand, within miaAat 4397.584 kb on + strand, within miaAat 4397.589 kb on - strand, within miaAat 4397.704 kb on - strand, within miaAat 4397.763 kb on - strand, within miaAat 4397.845 kb on - strand, within miaAat 4397.853 kb on + strand, within miaAat 4397.856 kb on + strand, within miaAat 4397.866 kb on + strand, within miaAat 4397.922 kb on + strand, within miaAat 4397.935 kb on + strand, within miaAat 4397.935 kb on + strand, within miaAat 4398.137 kb on + strandat 4398.137 kb on + strandat 4398.156 kb on + strandat 4398.233 kb on + strandat 4398.233 kb on + strandat 4398.433 kb on + strand, within hfqat 4398.562 kb on + strand, within hfqat 4398.565 kb on + strand, within hfqat 4398.593 kb on + strandat 4398.668 kb on - strandat 4398.675 kb on - strandat 4398.677 kb on + strandat 4398.677 kb on + strandat 4398.703 kb on + strandat 4398.714 kb on + strandat 4398.724 kb on - strandat 4398.728 kb on + strandat 4398.728 kb on + strandat 4398.793 kb on + strandat 4398.815 kb on - strandat 4398.815 kb on - strandat 4398.837 kb on + strand, within hflXat 4398.926 kb on - strand, within hflXat 4398.961 kb on + strand, within hflXat 4398.961 kb on + strand, within hflXat 4398.965 kb on + strand, within hflXat 4398.965 kb on + strand, within hflXat 4398.968 kb on - strand, within hflXat 4398.983 kb on - strand, within hflXat 4398.983 kb on - strand, within hflXat 4398.992 kb on - strand, within hflXat 4399.118 kb on + strand, within hflXat 4399.140 kb on + strand, within hflXat 4399.154 kb on + strand, within hflXat 4399.217 kb on - strand, within hflXat 4399.307 kb on + strand, within hflXat 4399.307 kb on + strand, within hflXat 4399.365 kb on - strand, within hflXat 4399.365 kb on - strand, within hflXat 4399.407 kb on + strand, within hflXat 4399.407 kb on + strand, within hflXat 4399.421 kb on - strand, within hflXat 4399.449 kb on - strand, within hflXat 4399.457 kb on - strand, within hflXat 4399.457 kb on - strand, within hflXat 4399.457 kb on - strand, within hflXat 4399.500 kb on - strand, within hflXat 4399.500 kb on - strand, within hflXat 4399.542 kb on + strand, within hflX

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep A; time point 1
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4,397,420 + miaA b4171 0.15 +0.9
4,397,449 + miaA b4171 0.18 +0.6
4,397,449 + miaA b4171 0.18 +0.6
4,397,455 + miaA b4171 0.19 +0.2
4,397,458 - miaA b4171 0.19 -0.5
4,397,505 + miaA b4171 0.24 +1.0
4,397,549 - miaA b4171 0.29 -0.7
4,397,584 + miaA b4171 0.32 +1.0
4,397,589 - miaA b4171 0.33 -0.3
4,397,704 - miaA b4171 0.45 +1.9
4,397,763 - miaA b4171 0.51 +1.0
4,397,845 - miaA b4171 0.60 +0.6
4,397,853 + miaA b4171 0.61 +1.0
4,397,856 + miaA b4171 0.61 -0.7
4,397,866 + miaA b4171 0.62 +0.4
4,397,922 + miaA b4171 0.68 -0.4
4,397,935 + miaA b4171 0.69 +0.4
4,397,935 + miaA b4171 0.69 +0.1
4,398,137 + +0.3
4,398,137 + +0.1
4,398,156 + -0.4
4,398,233 + -0.1
4,398,233 + -0.7
4,398,433 + hfq b4172 0.39 +2.6
4,398,562 + hfq b4172 0.81 +2.1
4,398,565 + hfq b4172 0.82 +1.4
4,398,593 + +1.3
4,398,668 - +0.2
4,398,675 - +0.7
4,398,677 + -0.2
4,398,677 + -0.1
4,398,703 + -0.1
4,398,714 + +0.9
4,398,724 - +0.3
4,398,728 + +0.1
4,398,728 + +0.4
4,398,793 + +0.4
4,398,815 - -0.9
4,398,815 - +0.9
4,398,837 + hflX b4173 0.11 -0.6
4,398,926 - hflX b4173 0.18 -0.7
4,398,961 + hflX b4173 0.21 -0.5
4,398,961 + hflX b4173 0.21 -0.3
4,398,965 + hflX b4173 0.21 +0.2
4,398,965 + hflX b4173 0.21 -0.3
4,398,968 - hflX b4173 0.21 +0.6
4,398,983 - hflX b4173 0.22 +0.0
4,398,983 - hflX b4173 0.22 -0.0
4,398,992 - hflX b4173 0.23 -2.0
4,399,118 + hflX b4173 0.33 +0.3
4,399,140 + hflX b4173 0.35 -0.3
4,399,154 + hflX b4173 0.36 +0.5
4,399,217 - hflX b4173 0.41 -0.1
4,399,307 + hflX b4173 0.48 +0.3
4,399,307 + hflX b4173 0.48 -0.4
4,399,365 - hflX b4173 0.52 +0.1
4,399,365 - hflX b4173 0.52 +0.4
4,399,407 + hflX b4173 0.56 -0.3
4,399,407 + hflX b4173 0.56 -1.5
4,399,421 - hflX b4173 0.57 +0.6
4,399,449 - hflX b4173 0.59 +0.4
4,399,457 - hflX b4173 0.59 +0.7
4,399,457 - hflX b4173 0.59 +0.8
4,399,457 - hflX b4173 0.59 +0.3
4,399,500 - hflX b4173 0.63 -0.7
4,399,500 - hflX b4173 0.63 -0.2
4,399,542 + hflX b4173 0.66 -0.4

Or see this region's nucleotide sequence