Experiment: Ying_OrganicAcid7 rep A; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rcsA and dsrB are separated by 43 nucleotides dsrB and b1953 are separated by 162 nucleotides b1953 and dsrA are separated by 13 nucleotides dsrA and yedP are separated by 197 nucleotides yedP and b1956 overlap by 4 nucleotides
b1951: rcsA - DNA-binding transcriptional activator, co-regulator with RcsB (NCBI), at 2,021,992 to 2,022,615
rcsA
b1952: dsrB - hypothetical protein (NCBI), at 2,022,659 to 2,022,847
dsrB
b1953: b1953 - orf, hypothetical protein (VIMSS), at 2,023,010 to 2,023,237
b1953
b1954: dsrA - regulatory antisense RNA (NCBI), at 2,023,251 to 2,023,337
dsrA
b1955: yedP - hypothetical protein (NCBI), at 2,023,535 to 2,024,350
yedP
b1956: b1956 - orf, hypothetical protein (VIMSS), at 2,024,347 to 2,026,041
b1956
Position (kb)
2023
2024
2025 Strain fitness (log2 ratio)
-2
-1
0
1 at 2022.610 kb on + strand at 2022.610 kb on + strand at 2022.627 kb on - strand at 2022.637 kb on - strand at 2022.650 kb on - strand at 2022.650 kb on - strand at 2022.674 kb on - strand at 2022.674 kb on - strand at 2022.694 kb on + strand, within dsrB at 2022.694 kb on + strand, within dsrB at 2022.720 kb on - strand, within dsrB at 2022.770 kb on + strand, within dsrB at 2022.770 kb on + strand, within dsrB at 2022.787 kb on - strand, within dsrB at 2022.787 kb on - strand, within dsrB at 2022.790 kb on + strand, within dsrB at 2022.792 kb on - strand, within dsrB at 2022.874 kb on + strand at 2022.874 kb on + strand at 2022.874 kb on + strand at 2022.940 kb on + strand at 2022.942 kb on + strand at 2023.038 kb on - strand, within b1953 at 2023.107 kb on + strand, within b1953 at 2023.138 kb on + strand, within b1953 at 2023.138 kb on + strand, within b1953 at 2023.138 kb on + strand, within b1953 at 2023.267 kb on - strand, within dsrA at 2023.298 kb on + strand, within dsrA at 2023.343 kb on - strand at 2023.343 kb on - strand at 2023.439 kb on - strand at 2023.439 kb on - strand at 2023.517 kb on + strand at 2023.525 kb on - strand at 2023.525 kb on - strand at 2023.540 kb on - strand at 2023.540 kb on - strand at 2023.604 kb on - strand at 2023.631 kb on - strand, within yedP at 2023.767 kb on - strand, within yedP at 2024.110 kb on + strand, within yedP at 2024.134 kb on + strand, within yedP at 2024.165 kb on + strand, within yedP at 2024.396 kb on + strand at 2024.459 kb on + strand at 2024.604 kb on + strand, within b1956 at 2024.869 kb on + strand, within b1956 at 2024.884 kb on - strand, within b1956 at 2024.884 kb on - strand, within b1956 at 2024.911 kb on - strand, within b1956 at 2025.014 kb on + strand, within b1956 at 2025.161 kb on + strand, within b1956 at 2025.161 kb on + strand, within b1956 at 2025.201 kb on - strand, within b1956 at 2025.201 kb on - strand, within b1956
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep A; time point 1 remove 2,022,610 + +1.2 2,022,610 + -0.4 2,022,627 - +0.4 2,022,637 - -0.3 2,022,650 - +0.3 2,022,650 - +0.6 2,022,674 - -0.6 2,022,674 - -0.9 2,022,694 + dsrB b1952 0.19 +1.1 2,022,694 + dsrB b1952 0.19 +0.7 2,022,720 - dsrB b1952 0.32 +0.4 2,022,770 + dsrB b1952 0.59 -0.2 2,022,770 + dsrB b1952 0.59 +0.5 2,022,787 - dsrB b1952 0.68 +0.1 2,022,787 - dsrB b1952 0.68 +0.2 2,022,790 + dsrB b1952 0.69 +0.4 2,022,792 - dsrB b1952 0.70 -0.1 2,022,874 + -0.3 2,022,874 + +0.1 2,022,874 + -0.7 2,022,940 + -0.2 2,022,942 + +0.4 2,023,038 - b1953 b1953 0.12 -0.2 2,023,107 + b1953 b1953 0.43 +0.2 2,023,138 + b1953 b1953 0.56 -0.2 2,023,138 + b1953 b1953 0.56 -0.1 2,023,138 + b1953 b1953 0.56 +0.4 2,023,267 - dsrA b1954 0.18 -0.5 2,023,298 + dsrA b1954 0.54 +0.6 2,023,343 - -0.1 2,023,343 - -0.1 2,023,439 - +0.0 2,023,439 - -0.8 2,023,517 + +0.3 2,023,525 - +1.0 2,023,525 - -0.1 2,023,540 - +0.0 2,023,540 - -0.3 2,023,604 - -0.8 2,023,631 - yedP b1955 0.12 +0.4 2,023,767 - yedP b1955 0.28 +1.2 2,024,110 + yedP b1955 0.70 -0.2 2,024,134 + yedP b1955 0.73 +0.5 2,024,165 + yedP b1955 0.77 +0.1 2,024,396 + +0.3 2,024,459 + +0.3 2,024,604 + b1956 b1956 0.15 +0.3 2,024,869 + b1956 b1956 0.31 -0.0 2,024,884 - b1956 b1956 0.32 +0.1 2,024,884 - b1956 b1956 0.32 +0.7 2,024,911 - b1956 b1956 0.33 +0.3 2,025,014 + b1956 b1956 0.39 -0.3 2,025,161 + b1956 b1956 0.48 -0.2 2,025,161 + b1956 b1956 0.48 -0.1 2,025,201 - b1956 b1956 0.50 +1.0 2,025,201 - b1956 b1956 0.50 -2.1
Or see this region's nucleotide sequence