Strain Fitness in Escherichia coli BW25113 around b1883

Experiment: Ying_OrganicAcid7 rep A; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcheZ and cheY are separated by 10 nucleotidescheY and cheB are separated by 14 nucleotidescheB and cheR are separated by 2 nucleotidescheR and tap are separated by 18 nucleotides b1881: cheZ - chemotaxis regulator, protein phosphatase for CheY (NCBI), at 1,964,417 to 1,965,061 cheZ b1882: cheY - chemotaxis regulator transmitting signal to flagellar motor component (NCBI), at 1,965,072 to 1,965,461 cheY b1883: cheB - chemotaxis-specific methylesterase (NCBI), at 1,965,476 to 1,966,525 cheB b1884: cheR - chemotaxis regulator, protein-glutamate methyltransferase (NCBI), at 1,966,528 to 1,967,388 cheR b1885: tap - methyl-accepting protein IV (NCBI), at 1,967,407 to 1,969,008 tap Position (kb) 1965 1966 1967Strain fitness (log2 ratio) -1 0 1at 1964.507 kb on - strand, within cheZat 1964.609 kb on - strand, within cheZat 1964.847 kb on - strand, within cheZat 1964.889 kb on + strand, within cheZat 1965.270 kb on + strand, within cheYat 1965.696 kb on - strand, within cheBat 1965.815 kb on - strand, within cheBat 1966.252 kb on + strand, within cheBat 1966.273 kb on + strand, within cheBat 1966.356 kb on - strand, within cheBat 1966.514 kb on + strandat 1966.553 kb on - strandat 1966.628 kb on + strand, within cheRat 1966.628 kb on + strand, within cheRat 1966.690 kb on - strand, within cheRat 1966.733 kb on + strand, within cheRat 1966.883 kb on - strand, within cheRat 1966.883 kb on - strand, within cheRat 1966.890 kb on - strand, within cheRat 1967.084 kb on - strand, within cheRat 1967.202 kb on + strand, within cheRat 1967.413 kb on - strandat 1967.413 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep A; time point 1
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1,964,507 - cheZ b1881 0.14 -0.2
1,964,609 - cheZ b1881 0.30 -0.1
1,964,847 - cheZ b1881 0.67 +0.1
1,964,889 + cheZ b1881 0.73 +0.0
1,965,270 + cheY b1882 0.51 +0.1
1,965,696 - cheB b1883 0.21 -0.1
1,965,815 - cheB b1883 0.32 +0.3
1,966,252 + cheB b1883 0.74 +0.9
1,966,273 + cheB b1883 0.76 +0.3
1,966,356 - cheB b1883 0.84 +0.5
1,966,514 + +0.2
1,966,553 - -0.9
1,966,628 + cheR b1884 0.12 -0.9
1,966,628 + cheR b1884 0.12 -0.2
1,966,690 - cheR b1884 0.19 +0.5
1,966,733 + cheR b1884 0.24 -0.1
1,966,883 - cheR b1884 0.41 -0.1
1,966,883 - cheR b1884 0.41 -0.2
1,966,890 - cheR b1884 0.42 +0.2
1,967,084 - cheR b1884 0.65 +0.7
1,967,202 + cheR b1884 0.78 +0.3
1,967,413 - +0.8
1,967,413 - +0.8

Or see this region's nucleotide sequence