Strain Fitness in Escherichia coli BW25113 around b0852

Experiment: Ying_OrganicAcid7 rep A; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntybjC and nfsA overlap by 17 nucleotidesnfsA and rimK are separated by 60 nucleotidesrimK and ybjN are separated by 87 nucleotidesybjN and potF are separated by 350 nucleotides b0850: ybjC - predicted inner membrane protein (NCBI), at 890,136 to 890,423 ybjC b0851: nfsA - nitroreductase A, NADPH-dependent, FMN-dependent (NCBI), at 890,407 to 891,129 nfsA b0852: rimK - ribosomal protein S6 modification protein (NCBI), at 891,190 to 892,092 rimK b0853: ybjN - predicted oxidoreductase (NCBI), at 892,180 to 892,656 ybjN b0854: potF - putrescine transporter subunit: periplasmic-binding component of ABC superfamily (NCBI), at 893,007 to 894,119 potF Position (kb) 891 892 893Strain fitness (log2 ratio) -1 0 1 2at 890.213 kb on + strand, within ybjCat 890.218 kb on + strand, within ybjCat 890.232 kb on - strand, within ybjCat 890.232 kb on - strand, within ybjCat 890.326 kb on + strand, within ybjCat 890.491 kb on - strand, within nfsAat 890.714 kb on - strand, within nfsAat 890.715 kb on - strand, within nfsAat 890.783 kb on + strand, within nfsAat 890.792 kb on - strand, within nfsAat 890.958 kb on + strand, within nfsAat 890.979 kb on + strand, within nfsAat 890.979 kb on + strand, within nfsAat 891.034 kb on + strand, within nfsAat 891.057 kb on - strandat 891.213 kb on + strandat 891.222 kb on + strandat 891.243 kb on + strandat 891.251 kb on + strandat 891.251 kb on + strandat 891.251 kb on + strandat 891.251 kb on + strandat 891.254 kb on + strandat 891.387 kb on - strand, within rimKat 891.387 kb on - strand, within rimKat 891.703 kb on - strand, within rimKat 891.703 kb on - strand, within rimKat 891.766 kb on - strand, within rimKat 891.868 kb on - strand, within rimKat 892.020 kb on - strandat 892.167 kb on + strandat 892.206 kb on + strandat 892.244 kb on - strand, within ybjNat 892.284 kb on - strand, within ybjNat 892.284 kb on - strand, within ybjNat 892.304 kb on + strand, within ybjNat 892.389 kb on - strand, within ybjNat 892.389 kb on - strand, within ybjNat 892.411 kb on + strand, within ybjNat 892.411 kb on + strand, within ybjNat 892.524 kb on + strand, within ybjNat 892.550 kb on - strand, within ybjNat 892.609 kb on - strandat 892.609 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep A; time point 1
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890,213 + ybjC b0850 0.27 -0.2
890,218 + ybjC b0850 0.28 -1.3
890,232 - ybjC b0850 0.33 +0.4
890,232 - ybjC b0850 0.33 +0.3
890,326 + ybjC b0850 0.66 +0.6
890,491 - nfsA b0851 0.12 +2.5
890,714 - nfsA b0851 0.42 -0.0
890,715 - nfsA b0851 0.43 -0.5
890,783 + nfsA b0851 0.52 -0.5
890,792 - nfsA b0851 0.53 -0.4
890,958 + nfsA b0851 0.76 -0.5
890,979 + nfsA b0851 0.79 -1.0
890,979 + nfsA b0851 0.79 +0.0
891,034 + nfsA b0851 0.87 -0.5
891,057 - -0.2
891,213 + +0.7
891,222 + +0.9
891,243 + +0.6
891,251 + +0.8
891,251 + +0.2
891,251 + +0.5
891,251 + +0.7
891,254 + +0.6
891,387 - rimK b0852 0.22 +0.2
891,387 - rimK b0852 0.22 +0.2
891,703 - rimK b0852 0.57 +0.7
891,703 - rimK b0852 0.57 +0.6
891,766 - rimK b0852 0.64 +0.0
891,868 - rimK b0852 0.75 +0.4
892,020 - +0.6
892,167 + -0.1
892,206 + -0.5
892,244 - ybjN b0853 0.13 +0.7
892,284 - ybjN b0853 0.22 +0.0
892,284 - ybjN b0853 0.22 -0.0
892,304 + ybjN b0853 0.26 +0.1
892,389 - ybjN b0853 0.44 +0.1
892,389 - ybjN b0853 0.44 -0.5
892,411 + ybjN b0853 0.48 -0.3
892,411 + ybjN b0853 0.48 +0.4
892,524 + ybjN b0853 0.72 -0.2
892,550 - ybjN b0853 0.78 -0.8
892,609 - +0.7
892,609 - +1.0

Or see this region's nucleotide sequence