Experiment: Ying_OrganicAcid7 rep A; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nfnB and ybdF are separated by 93 nucleotides ybdF and ybdJ are separated by 64 nucleotides ybdJ and ybdK are separated by 65 nucleotides
b0578: nfnB - dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive (NCBI), at 603,994 to 604,647
nfnB
b0579: ybdF - hypothetical protein (NCBI), at 604,741 to 605,109
ybdF
b0580: ybdJ - predicted inner membrane protein (NCBI), at 605,174 to 605,422
ybdJ
b0581: ybdK - gamma-glutamyl:cysteine ligase (NCBI), at 605,488 to 606,606
ybdK
Position (kb) 605
606 Strain fitness (log2 ratio) -1
0
1 at 604.210 kb on + strand, within nfnB at 604.210 kb on + strand, within nfnB at 604.249 kb on + strand, within nfnB at 604.347 kb on + strand, within nfnB at 604.404 kb on - strand, within nfnB at 604.425 kb on - strand, within nfnB at 604.488 kb on - strand, within nfnB at 604.488 kb on - strand, within nfnB at 604.488 kb on - strand, within nfnB at 604.538 kb on + strand, within nfnB at 604.540 kb on + strand, within nfnB at 604.777 kb on - strand at 604.871 kb on + strand, within ybdF at 604.888 kb on - strand, within ybdF at 604.894 kb on - strand, within ybdF at 605.086 kb on + strand at 605.180 kb on - strand at 605.180 kb on - strand at 605.335 kb on + strand, within ybdJ at 605.355 kb on + strand, within ybdJ at 605.355 kb on + strand, within ybdJ at 605.472 kb on - strand at 605.498 kb on + strand at 605.549 kb on - strand at 605.683 kb on - strand, within ybdK at 605.699 kb on + strand, within ybdK at 605.713 kb on - strand, within ybdK at 605.713 kb on - strand, within ybdK at 605.725 kb on - strand, within ybdK at 605.754 kb on - strand, within ybdK at 605.862 kb on + strand, within ybdK at 605.862 kb on + strand, within ybdK at 605.877 kb on - strand, within ybdK at 605.918 kb on - strand, within ybdK at 605.953 kb on + strand, within ybdK at 605.966 kb on + strand, within ybdK at 606.060 kb on + strand, within ybdK at 606.095 kb on + strand, within ybdK at 606.095 kb on + strand, within ybdK at 606.317 kb on - strand, within ybdK at 606.367 kb on - strand, within ybdK
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep A; time point 1 remove 604,210 + nfnB b0578 0.33 -0.6 604,210 + nfnB b0578 0.33 +0.9 604,249 + nfnB b0578 0.39 -0.3 604,347 + nfnB b0578 0.54 -1.2 604,404 - nfnB b0578 0.63 +0.7 604,425 - nfnB b0578 0.66 -0.4 604,488 - nfnB b0578 0.76 -0.2 604,488 - nfnB b0578 0.76 -0.1 604,488 - nfnB b0578 0.76 -1.4 604,538 + nfnB b0578 0.83 -0.1 604,540 + nfnB b0578 0.83 -0.4 604,777 - +0.3 604,871 + ybdF b0579 0.35 +0.1 604,888 - ybdF b0579 0.40 +0.2 604,894 - ybdF b0579 0.41 -0.0 605,086 + -0.4 605,180 - +0.8 605,180 - -0.0 605,335 + ybdJ b0580 0.65 +0.3 605,355 + ybdJ b0580 0.73 +0.1 605,355 + ybdJ b0580 0.73 +0.9 605,472 - +0.6 605,498 + +0.0 605,549 - +0.2 605,683 - ybdK b0581 0.17 +0.1 605,699 + ybdK b0581 0.19 -0.4 605,713 - ybdK b0581 0.20 +0.0 605,713 - ybdK b0581 0.20 +0.3 605,725 - ybdK b0581 0.21 +0.6 605,754 - ybdK b0581 0.24 +0.2 605,862 + ybdK b0581 0.33 -0.7 605,862 + ybdK b0581 0.33 -0.2 605,877 - ybdK b0581 0.35 -0.5 605,918 - ybdK b0581 0.38 +0.3 605,953 + ybdK b0581 0.42 +0.5 605,966 + ybdK b0581 0.43 -0.4 606,060 + ybdK b0581 0.51 -0.6 606,095 + ybdK b0581 0.54 -0.1 606,095 + ybdK b0581 0.54 +0.6 606,317 - ybdK b0581 0.74 +0.1 606,367 - ybdK b0581 0.79 -0.2
Or see this region's nucleotide sequence