Experiment: Ying_OrganicAcid7 rep A; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mraZ and mraW are separated by 1 nucleotides mraW and ftsL overlap by 4 nucleotides ftsL and ftsI are separated by 15 nucleotides
b0081: mraZ - hypothetical protein (NCBI), at 89,634 to 90,092
mraZ
b0082: mraW - S-adenosyl-methyltransferase (NCBI), at 90,094 to 91,035
mraW
b0083: ftsL - membrane bound cell division protein at septum containing leucine zipper motif (NCBI), at 91,032 to 91,397
ftsL
b0084: ftsI - transpeptidase involved in septal peptidoglycan synthesis (penicillin-binding protein 3) (NCBI), at 91,413 to 93,179
ftsI
Position (kb)
90
91
92 Strain fitness (log2 ratio)
-1
0
1 at 89.122 kb on + strand at 89.132 kb on + strand at 89.225 kb on + strand at 89.294 kb on - strand at 89.307 kb on + strand at 89.307 kb on + strand at 89.312 kb on + strand at 89.312 kb on + strand at 89.312 kb on + strand at 89.313 kb on - strand at 89.315 kb on - strand at 89.325 kb on + strand at 89.325 kb on - strand at 89.330 kb on - strand at 89.391 kb on + strand at 89.393 kb on + strand at 89.401 kb on - strand at 89.411 kb on + strand at 89.411 kb on + strand at 89.416 kb on - strand at 89.508 kb on + strand at 89.508 kb on + strand at 89.542 kb on - strand at 89.542 kb on - strand at 89.550 kb on + strand at 89.558 kb on - strand at 89.649 kb on + strand at 89.649 kb on + strand at 89.658 kb on + strand at 89.680 kb on + strand, within mraZ at 89.685 kb on + strand, within mraZ at 89.917 kb on + strand, within mraZ at 89.917 kb on + strand, within mraZ at 89.981 kb on + strand, within mraZ at 90.039 kb on + strand, within mraZ at 90.039 kb on + strand, within mraZ at 90.042 kb on + strand, within mraZ at 90.042 kb on + strand, within mraZ at 90.230 kb on + strand, within mraW at 90.473 kb on + strand, within mraW at 90.518 kb on + strand, within mraW at 90.544 kb on + strand, within mraW at 90.681 kb on + strand, within mraW at 90.701 kb on + strand, within mraW at 90.783 kb on + strand, within mraW at 90.887 kb on + strand, within mraW at 90.896 kb on + strand, within mraW at 90.899 kb on + strand, within mraW at 90.979 kb on + strand at 91.008 kb on + strand at 91.020 kb on + strand at 91.020 kb on + strand at 91.020 kb on + strand at 91.028 kb on + strand at 91.028 kb on + strand at 91.357 kb on + strand at 91.362 kb on + strand at 91.378 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep A; time point 1 remove 89,122 + +0.2 89,132 + -0.6 89,225 + -0.7 89,294 - +0.3 89,307 + +0.1 89,307 + -0.2 89,312 + -0.2 89,312 + -0.1 89,312 + -0.1 89,313 - +0.4 89,315 - -0.4 89,325 + +0.6 89,325 - -1.0 89,330 - -0.2 89,391 + +0.1 89,393 + +0.6 89,401 - +0.1 89,411 + -0.1 89,411 + -0.7 89,416 - -0.3 89,508 + +0.6 89,508 + +0.7 89,542 - +0.0 89,542 - -0.7 89,550 + +0.1 89,558 - -0.7 89,649 + +0.1 89,649 + +0.2 89,658 + +0.4 89,680 + mraZ b0081 0.10 -0.4 89,685 + mraZ b0081 0.11 -0.1 89,917 + mraZ b0081 0.62 -0.9 89,917 + mraZ b0081 0.62 -0.4 89,981 + mraZ b0081 0.76 -0.2 90,039 + mraZ b0081 0.88 +0.2 90,039 + mraZ b0081 0.88 -0.3 90,042 + mraZ b0081 0.89 -0.5 90,042 + mraZ b0081 0.89 -0.1 90,230 + mraW b0082 0.14 -0.2 90,473 + mraW b0082 0.40 +1.1 90,518 + mraW b0082 0.45 +0.5 90,544 + mraW b0082 0.48 -0.5 90,681 + mraW b0082 0.62 +0.7 90,701 + mraW b0082 0.64 +1.2 90,783 + mraW b0082 0.73 +1.0 90,887 + mraW b0082 0.84 +1.1 90,896 + mraW b0082 0.85 +0.2 90,899 + mraW b0082 0.85 +1.7 90,979 + +1.4 91,008 + +0.1 91,020 + -0.9 91,020 + +0.9 91,020 + -0.6 91,028 + -0.1 91,028 + -0.3 91,357 + +1.6 91,362 + +0.3 91,378 + -0.2
Or see this region's nucleotide sequence