Strain Fitness in Escherichia coli BW25113 around b4426

Experiment: Ying_AminoAcid20 rep A; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntabgR and isrA are separated by 2 nucleotidesisrA and ydaL are separated by 169 nucleotidesydaL and b1341 are separated by 20 nucleotides b1339: abgR - predicted DNA-binding transcriptional regulator (NCBI), at 1,402,765 to 1,403,673 abgR b4426: isrA - unknown RNA (NCBI), at 1,403,676 to 1,403,833 isrA b1340: ydaL - hypothetical protein (NCBI), at 1,404,003 to 1,404,566 ydaL b1341: b1341 - orf, hypothetical protein (VIMSS), at 1,404,587 to 1,405,819 b1341 Position (kb) 1403 1404Strain fitness (log2 ratio) -1 0 1at 1402.834 kb on - strandat 1402.988 kb on + strand, within abgRat 1403.075 kb on + strand, within abgRat 1403.075 kb on + strand, within abgRat 1403.087 kb on + strand, within abgRat 1403.087 kb on + strand, within abgRat 1403.109 kb on - strand, within abgRat 1403.119 kb on - strand, within abgRat 1403.119 kb on - strand, within abgRat 1403.153 kb on - strand, within abgRat 1403.153 kb on - strand, within abgRat 1403.464 kb on - strand, within abgRat 1403.464 kb on - strand, within abgRat 1403.519 kb on - strand, within abgRat 1403.519 kb on - strand, within abgRat 1403.720 kb on + strand, within isrAat 1403.720 kb on + strand, within isrAat 1403.732 kb on - strand, within isrAat 1403.735 kb on - strand, within isrAat 1404.417 kb on - strand, within ydaLat 1404.648 kb on + strandat 1404.666 kb on - strandat 1404.686 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 1
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1,402,834 - +0.4
1,402,988 + abgR b1339 0.25 +0.2
1,403,075 + abgR b1339 0.34 +0.0
1,403,075 + abgR b1339 0.34 +0.9
1,403,087 + abgR b1339 0.35 -0.7
1,403,087 + abgR b1339 0.35 +0.4
1,403,109 - abgR b1339 0.38 +1.1
1,403,119 - abgR b1339 0.39 +0.4
1,403,119 - abgR b1339 0.39 +0.7
1,403,153 - abgR b1339 0.43 -0.1
1,403,153 - abgR b1339 0.43 +0.0
1,403,464 - abgR b1339 0.77 +0.3
1,403,464 - abgR b1339 0.77 -0.4
1,403,519 - abgR b1339 0.83 +1.1
1,403,519 - abgR b1339 0.83 +0.4
1,403,720 + isrA b4426 0.28 +0.7
1,403,720 + isrA b4426 0.28 +0.2
1,403,732 - isrA b4426 0.35 +0.7
1,403,735 - isrA b4426 0.37 +0.4
1,404,417 - ydaL b1340 0.73 +1.3
1,404,648 + +1.0
1,404,666 - -0.1
1,404,686 - -0.2

Or see this region's nucleotide sequence