Strain Fitness in Escherichia coli BW25113 around b4294

Experiment: Ying_AminoAcid20 rep A; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfecR and fecI overlap by 4 nucleotidesfecI and insA-7 are separated by 291 nucleotidesinsA-7 and insB-7 overlap by 82 nucleotidesinsB-7 and yjhU are separated by 113 nucleotides b4292: fecR - KpLE2 phage-like element; transmembrane signal transducer for ferric citrate transport (NCBI), at 4,514,787 to 4,515,740 fecR b4293: fecI - KpLE2 phage-like element; RNA polymerase, sigma 19 factor (NCBI), at 4,515,737 to 4,516,258 fecI b4294: insA-7 - KpLE2 phage-like element; IS1 repressor protein InsA (NCBI), at 4,516,550 to 4,516,825 insA-7 b4576: insB-7 - no description, at 4,516,744 to 4,517,247 insB-7 b4295: yjhU - orf, hypothetical protein (VIMSS), at 4,517,361 to 4,518,347 yjhU Position (kb) 4516 4517Strain fitness (log2 ratio) -1 0 1at 4515.584 kb on - strand, within fecRat 4515.609 kb on - strand, within fecRat 4515.647 kb on + strandat 4515.669 kb on + strandat 4515.669 kb on + strandat 4515.702 kb on - strandat 4515.749 kb on + strandat 4515.756 kb on - strandat 4515.760 kb on + strandat 4515.770 kb on + strandat 4515.852 kb on - strand, within fecIat 4515.928 kb on - strand, within fecIat 4515.928 kb on - strand, within fecIat 4515.997 kb on + strand, within fecIat 4516.053 kb on + strand, within fecIat 4516.053 kb on + strand, within fecIat 4516.059 kb on + strand, within fecIat 4516.059 kb on + strand, within fecIat 4516.067 kb on - strand, within fecIat 4516.067 kb on - strand, within fecIat 4516.111 kb on - strand, within fecIat 4516.116 kb on - strand, within fecIat 4516.135 kb on - strand, within fecIat 4516.377 kb on + strandat 4516.417 kb on + strandat 4516.430 kb on + strandat 4516.456 kb on + strandat 4516.515 kb on + strandat 4516.515 kb on + strandat 4516.686 kb on - strand, within insA-7at 4516.686 kb on - strand, within insA-7at 4516.884 kb on + strand, within insB-7at 4516.946 kb on + strand, within insB-7at 4516.959 kb on - strand, within insB-7at 4516.983 kb on + strand, within insB-7at 4516.983 kb on + strand, within insB-7at 4517.038 kb on - strand, within insB-7at 4517.038 kb on - strand, within insB-7at 4517.064 kb on - strand, within insB-7at 4517.088 kb on + strand, within insB-7at 4517.100 kb on + strand, within insB-7at 4517.108 kb on - strand, within insB-7at 4517.146 kb on + strand, within insB-7at 4517.146 kb on + strand, within insB-7at 4517.333 kb on + strandat 4517.341 kb on - strandat 4517.341 kb on - strandat 4517.341 kb on - strandat 4517.341 kb on - strandat 4517.377 kb on - strandat 4517.434 kb on + strandat 4517.439 kb on - strandat 4517.539 kb on - strand, within yjhUat 4517.710 kb on - strand, within yjhUat 4517.710 kb on - strand, within yjhUat 4517.738 kb on + strand, within yjhUat 4517.750 kb on + strand, within yjhUat 4517.778 kb on - strand, within yjhUat 4517.806 kb on - strand, within yjhUat 4517.806 kb on - strand, within yjhU

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 1
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4,515,584 - fecR b4292 0.84 +0.5
4,515,609 - fecR b4292 0.86 +0.1
4,515,647 + -0.0
4,515,669 + -0.5
4,515,669 + +0.3
4,515,702 - -0.5
4,515,749 + +0.2
4,515,756 - +0.1
4,515,760 + +0.0
4,515,770 + -0.6
4,515,852 - fecI b4293 0.22 +0.8
4,515,928 - fecI b4293 0.37 -0.0
4,515,928 - fecI b4293 0.37 -1.1
4,515,997 + fecI b4293 0.50 -1.7
4,516,053 + fecI b4293 0.61 +1.4
4,516,053 + fecI b4293 0.61 -0.3
4,516,059 + fecI b4293 0.62 -0.2
4,516,059 + fecI b4293 0.62 -0.2
4,516,067 - fecI b4293 0.63 +0.1
4,516,067 - fecI b4293 0.63 -0.3
4,516,111 - fecI b4293 0.72 -0.3
4,516,116 - fecI b4293 0.73 -1.5
4,516,135 - fecI b4293 0.76 -0.6
4,516,377 + +0.0
4,516,417 + -0.6
4,516,430 + +0.4
4,516,456 + -0.8
4,516,515 + -0.5
4,516,515 + -0.6
4,516,686 - insA-7 b4294 0.49 +0.2
4,516,686 - insA-7 b4294 0.49 +0.5
4,516,884 + insB-7 b4576 0.28 -0.6
4,516,946 + insB-7 b4576 0.40 -0.3
4,516,959 - insB-7 b4576 0.43 -0.2
4,516,983 + insB-7 b4576 0.47 -0.3
4,516,983 + insB-7 b4576 0.47 -0.4
4,517,038 - insB-7 b4576 0.58 +0.1
4,517,038 - insB-7 b4576 0.58 +0.8
4,517,064 - insB-7 b4576 0.63 -0.5
4,517,088 + insB-7 b4576 0.68 -0.3
4,517,100 + insB-7 b4576 0.71 +0.5
4,517,108 - insB-7 b4576 0.72 +0.7
4,517,146 + insB-7 b4576 0.80 +0.1
4,517,146 + insB-7 b4576 0.80 +0.8
4,517,333 + +0.3
4,517,341 - +0.2
4,517,341 - -0.5
4,517,341 - +0.1
4,517,341 - -0.5
4,517,377 - +0.2
4,517,434 + +0.2
4,517,439 - -0.4
4,517,539 - yjhU b4295 0.18 +0.8
4,517,710 - yjhU b4295 0.35 +1.4
4,517,710 - yjhU b4295 0.35 -0.5
4,517,738 + yjhU b4295 0.38 -0.0
4,517,750 + yjhU b4295 0.39 -0.1
4,517,778 - yjhU b4295 0.42 +0.4
4,517,806 - yjhU b4295 0.45 +0.3
4,517,806 - yjhU b4295 0.45 +0.1

Or see this region's nucleotide sequence