Strain Fitness in Escherichia coli BW25113 around b1926

Experiment: Ying_AminoAcid20 rep A; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfliD and fliS are separated by 24 nucleotidesfliS and fliT overlap by 1 nucleotidesfliT and amyA are separated by 77 nucleotides b1924: fliD - flagellar capping protein (NCBI), at 2,001,896 to 2,003,302 fliD b1925: fliS - flagellar protein FliS (NCBI), at 2,003,327 to 2,003,737 fliS b1926: fliT - predicted chaperone (NCBI), at 2,003,737 to 2,004,102 fliT b1927: amyA - cytoplasmic alpha-amylase (NCBI), at 2,004,180 to 2,005,667 amyA Position (kb) 2003 2004 2005Strain fitness (log2 ratio) -3 -2 -1 0 1at 2002.829 kb on - strand, within fliDat 2002.892 kb on - strand, within fliDat 2002.894 kb on - strand, within fliDat 2002.894 kb on - strand, within fliDat 2002.913 kb on - strand, within fliDat 2003.080 kb on - strand, within fliDat 2003.414 kb on + strand, within fliSat 2003.451 kb on - strand, within fliSat 2003.451 kb on - strand, within fliSat 2003.451 kb on - strand, within fliSat 2003.462 kb on + strand, within fliSat 2003.544 kb on + strand, within fliSat 2003.544 kb on + strand, within fliSat 2003.699 kb on + strandat 2003.734 kb on + strandat 2003.735 kb on - strandat 2003.738 kb on - strandat 2003.923 kb on - strand, within fliTat 2003.933 kb on - strand, within fliTat 2004.035 kb on + strand, within fliTat 2004.073 kb on + strandat 2004.135 kb on + strandat 2004.148 kb on + strandat 2004.241 kb on + strandat 2004.241 kb on + strandat 2004.249 kb on - strandat 2004.249 kb on - strandat 2004.249 kb on - strandat 2004.299 kb on + strandat 2004.446 kb on + strand, within amyAat 2004.461 kb on - strand, within amyAat 2004.533 kb on + strand, within amyAat 2004.543 kb on - strand, within amyAat 2004.614 kb on - strand, within amyAat 2004.614 kb on - strand, within amyAat 2004.739 kb on - strand, within amyAat 2004.809 kb on + strand, within amyAat 2004.861 kb on - strand, within amyAat 2004.942 kb on - strand, within amyAat 2005.017 kb on - strand, within amyA

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 1
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2,002,829 - fliD b1924 0.66 -0.3
2,002,892 - fliD b1924 0.71 -0.4
2,002,894 - fliD b1924 0.71 +0.1
2,002,894 - fliD b1924 0.71 +0.7
2,002,913 - fliD b1924 0.72 +1.0
2,003,080 - fliD b1924 0.84 -0.5
2,003,414 + fliS b1925 0.21 -0.0
2,003,451 - fliS b1925 0.30 -0.3
2,003,451 - fliS b1925 0.30 +0.8
2,003,451 - fliS b1925 0.30 +0.0
2,003,462 + fliS b1925 0.33 -0.3
2,003,544 + fliS b1925 0.53 +0.8
2,003,544 + fliS b1925 0.53 +0.4
2,003,699 + +0.1
2,003,734 + +0.3
2,003,735 - -0.5
2,003,738 - +0.0
2,003,923 - fliT b1926 0.51 +0.4
2,003,933 - fliT b1926 0.54 +1.0
2,004,035 + fliT b1926 0.81 +0.3
2,004,073 + -0.4
2,004,135 + +0.7
2,004,148 + +0.4
2,004,241 + +0.0
2,004,241 + +0.0
2,004,249 - +0.1
2,004,249 - +0.3
2,004,249 - -0.3
2,004,299 + -0.4
2,004,446 + amyA b1927 0.18 -0.8
2,004,461 - amyA b1927 0.19 -0.1
2,004,533 + amyA b1927 0.24 +0.4
2,004,543 - amyA b1927 0.24 -0.0
2,004,614 - amyA b1927 0.29 -0.7
2,004,614 - amyA b1927 0.29 -0.9
2,004,739 - amyA b1927 0.38 -1.0
2,004,809 + amyA b1927 0.42 -0.5
2,004,861 - amyA b1927 0.46 +0.4
2,004,942 - amyA b1927 0.51 -0.6
2,005,017 - amyA b1927 0.56 -2.8

Or see this region's nucleotide sequence