Strain Fitness in Escherichia coli BW25113 around b1758

Experiment: Ying_AminoAcid20 rep A; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntb1757 and b1758 are separated by 8 nucleotidesb1758 and nudG are separated by 86 nucleotidesnudG and ynjH overlap by 35 nucleotidesynjH and gdhA are separated by 235 nucleotides b1757: b1757 - putative thiosulfate sulfur transferase (VIMSS), at 1,837,491 to 1,838,798 b1757 b1758: b1758 - putative cytochrome oxidase (VIMSS), at 1,838,807 to 1,839,427 b1758 b1759: nudG - pyrimidine (deoxy)nucleoside triphosphate pyrophosphohydrolase (NCBI), at 1,839,514 to 1,839,921 nudG b1760: ynjH - hypothetical protein (NCBI), at 1,839,887 to 1,840,159 ynjH b1761: gdhA - glutamate dehydrogenase (NCBI), at 1,840,395 to 1,841,738 gdhA Position (kb) 1838 1839 1840Strain fitness (log2 ratio) -1 0 1at 1838.008 kb on - strand, within b1757at 1838.022 kb on + strand, within b1757at 1838.065 kb on - strand, within b1757at 1838.179 kb on + strand, within b1757at 1838.194 kb on - strand, within b1757at 1838.463 kb on + strand, within b1757at 1838.563 kb on + strand, within b1757at 1838.833 kb on + strandat 1838.833 kb on + strandat 1839.201 kb on + strand, within b1758at 1839.552 kb on - strandat 1839.552 kb on - strandat 1839.760 kb on - strand, within nudGat 1839.893 kb on - strandat 1840.011 kb on + strand, within ynjHat 1840.026 kb on - strand, within ynjHat 1840.029 kb on - strand, within ynjHat 1840.031 kb on - strand, within ynjHat 1840.258 kb on - strandat 1840.325 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 1
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1,838,008 - b1757 b1757 0.40 +1.0
1,838,022 + b1757 b1757 0.41 +0.4
1,838,065 - b1757 b1757 0.44 -0.0
1,838,179 + b1757 b1757 0.53 +0.6
1,838,194 - b1757 b1757 0.54 -1.0
1,838,463 + b1757 b1757 0.74 -1.7
1,838,563 + b1757 b1757 0.82 -0.1
1,838,833 + +0.7
1,838,833 + +0.7
1,839,201 + b1758 b1758 0.63 +0.6
1,839,552 - +0.3
1,839,552 - +0.9
1,839,760 - nudG b1759 0.60 +0.1
1,839,893 - -0.9
1,840,011 + ynjH b1760 0.45 -0.8
1,840,026 - ynjH b1760 0.51 -0.2
1,840,029 - ynjH b1760 0.52 -0.3
1,840,031 - ynjH b1760 0.53 -0.2
1,840,258 - +0.8
1,840,325 - -0.3

Or see this region's nucleotide sequence