Strain Fitness in Escherichia coli BW25113 around b1644

Experiment: Ying_AminoAcid20 rep A; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntslyB and slyA are separated by 46 nucleotidesslyA and ydhI are separated by 200 nucleotidesydhI and b1644 are separated by 2 nucleotidesb1644 and ydhK overlap by 1 nucleotides b1641: slyB - outer membrane lipoprotein (NCBI), at 1,717,900 to 1,718,367 slyB b1642: slyA - transcriptional regulator for cryptic hemolysin (VIMSS), at 1,718,414 to 1,718,848 slyA b1643: ydhI - predicted inner membrane protein (NCBI), at 1,719,049 to 1,719,285 ydhI b1644: b1644 - putative membrane protein (VIMSS), at 1,719,288 to 1,720,145 b1644 b1645: ydhK - conserved inner membrane protein (NCBI), at 1,720,145 to 1,722,157 ydhK Position (kb) 1719 1720 1721Strain fitness (log2 ratio) -1 0 1at 1718.718 kb on - strand, within slyAat 1718.718 kb on - strand, within slyAat 1718.809 kb on - strandat 1719.062 kb on - strandat 1719.065 kb on + strandat 1719.078 kb on + strand, within ydhIat 1719.103 kb on - strand, within ydhIat 1719.163 kb on + strand, within ydhIat 1719.171 kb on - strand, within ydhIat 1719.171 kb on - strand, within ydhIat 1719.190 kb on + strand, within ydhIat 1719.211 kb on + strand, within ydhIat 1719.221 kb on - strand, within ydhIat 1719.559 kb on - strand, within b1644at 1719.779 kb on + strand, within b1644at 1719.779 kb on + strand, within b1644at 1719.786 kb on - strand, within b1644at 1719.786 kb on - strand, within b1644at 1719.832 kb on - strand, within b1644at 1719.832 kb on - strand, within b1644at 1720.086 kb on + strandat 1720.196 kb on - strandat 1720.263 kb on + strandat 1720.263 kb on + strandat 1720.388 kb on + strand, within ydhKat 1720.466 kb on + strand, within ydhKat 1720.466 kb on + strand, within ydhKat 1720.466 kb on + strand, within ydhKat 1720.466 kb on + strand, within ydhKat 1720.520 kb on + strand, within ydhKat 1720.587 kb on - strand, within ydhKat 1720.587 kb on - strand, within ydhKat 1720.615 kb on + strand, within ydhKat 1720.741 kb on + strand, within ydhKat 1721.132 kb on + strand, within ydhKat 1721.142 kb on - strand, within ydhKat 1721.142 kb on - strand, within ydhK

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 1
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1,718,718 - slyA b1642 0.70 -0.3
1,718,718 - slyA b1642 0.70 +0.4
1,718,809 - +0.6
1,719,062 - -0.3
1,719,065 + +1.3
1,719,078 + ydhI b1643 0.12 +0.4
1,719,103 - ydhI b1643 0.23 +0.3
1,719,163 + ydhI b1643 0.48 +0.5
1,719,171 - ydhI b1643 0.51 -0.0
1,719,171 - ydhI b1643 0.51 +0.6
1,719,190 + ydhI b1643 0.59 +0.1
1,719,211 + ydhI b1643 0.68 -0.2
1,719,221 - ydhI b1643 0.73 -0.6
1,719,559 - b1644 b1644 0.32 -0.0
1,719,779 + b1644 b1644 0.57 +0.3
1,719,779 + b1644 b1644 0.57 +0.7
1,719,786 - b1644 b1644 0.58 +0.3
1,719,786 - b1644 b1644 0.58 +0.6
1,719,832 - b1644 b1644 0.63 +0.0
1,719,832 - b1644 b1644 0.63 +0.8
1,720,086 + -1.3
1,720,196 - +0.2
1,720,263 + -0.8
1,720,263 + -0.4
1,720,388 + ydhK b1645 0.12 -0.3
1,720,466 + ydhK b1645 0.16 +0.1
1,720,466 + ydhK b1645 0.16 +0.2
1,720,466 + ydhK b1645 0.16 -0.5
1,720,466 + ydhK b1645 0.16 -0.1
1,720,520 + ydhK b1645 0.19 -0.3
1,720,587 - ydhK b1645 0.22 +0.5
1,720,587 - ydhK b1645 0.22 +0.7
1,720,615 + ydhK b1645 0.23 +0.0
1,720,741 + ydhK b1645 0.30 +0.4
1,721,132 + ydhK b1645 0.49 -0.3
1,721,142 - ydhK b1645 0.50 -0.0
1,721,142 - ydhK b1645 0.50 +0.2

Or see this region's nucleotide sequence