Strain Fitness in Escherichia coli BW25113 around b1351

Experiment: Ying_AminoAcid20 rep A; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrecE and racC are separated by 101 nucleotidesracC and ydaE are separated by 74 nucleotidesydaE and ydaD overlap by 1 nucleotidesydaD and sieB are separated by 441 nucleotides b1350: recE - Rac prophage; exonuclease VIII, 5' -> 3' specific dsDNA exonuclease (NCBI), at 1,412,810 to 1,415,410 recE b1351: racC - Rac prophage; predicted protein (NCBI), at 1,415,512 to 1,415,787 racC b4526: ydaE - Rac prophage; conserved protein (NCBI), at 1,415,862 to 1,416,032 ydaE b1352: ydaD - orf, hypothetical protein (VIMSS), at 1,416,032 to 1,416,253 ydaD b1353: sieB - phage superinfection exclusion protein (VIMSS), at 1,416,695 to 1,417,183 sieB Position (kb) 1415 1416Strain fitness (log2 ratio) -1 0 1 2at 1414.602 kb on - strand, within recEat 1414.633 kb on - strand, within recEat 1414.633 kb on - strand, within recEat 1414.719 kb on - strand, within recEat 1414.747 kb on + strand, within recEat 1414.758 kb on + strand, within recEat 1414.758 kb on + strand, within recEat 1415.046 kb on + strand, within recEat 1415.106 kb on + strand, within recEat 1415.187 kb on + strandat 1415.187 kb on + strandat 1415.195 kb on - strandat 1415.195 kb on - strandat 1415.195 kb on - strandat 1415.300 kb on + strandat 1415.416 kb on - strandat 1415.468 kb on + strandat 1415.468 kb on + strandat 1415.468 kb on + strandat 1415.468 kb on + strandat 1415.468 kb on + strandat 1415.468 kb on + strandat 1415.484 kb on + strandat 1415.538 kb on + strandat 1415.620 kb on + strand, within racCat 1415.625 kb on - strand, within racCat 1415.649 kb on - strand, within racCat 1416.473 kb on + strandat 1416.624 kb on - strandat 1416.644 kb on - strandat 1416.679 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 1
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1,414,602 - recE b1350 0.69 -0.0
1,414,633 - recE b1350 0.70 +0.2
1,414,633 - recE b1350 0.70 -0.4
1,414,719 - recE b1350 0.73 -1.3
1,414,747 + recE b1350 0.74 -0.0
1,414,758 + recE b1350 0.75 +0.4
1,414,758 + recE b1350 0.75 +0.7
1,415,046 + recE b1350 0.86 +0.1
1,415,106 + recE b1350 0.88 -0.1
1,415,187 + +0.7
1,415,187 + +0.3
1,415,195 - +0.3
1,415,195 - +0.4
1,415,195 - +0.6
1,415,300 + -0.0
1,415,416 - +0.7
1,415,468 + +0.3
1,415,468 + +0.6
1,415,468 + -0.5
1,415,468 + +0.0
1,415,468 + +0.4
1,415,468 + +0.8
1,415,484 + -0.5
1,415,538 + -0.0
1,415,620 + racC b1351 0.39 +0.7
1,415,625 - racC b1351 0.41 +2.7
1,415,649 - racC b1351 0.50 +2.3
1,416,473 + +2.3
1,416,624 - +2.3
1,416,644 - -0.5
1,416,679 - +2.3

Or see this region's nucleotide sequence