Strain Fitness in Escherichia coli BW25113 around b4541

Experiment: LB Anaerobic Allura Red AC 250 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyehI and yehK are separated by 60 nucleotidesyehK and yehL are separated by 306 nucleotides b2118: yehI - hypothetical protein (NCBI), at 2,198,301 to 2,201,933 yehI b4541: yehK - hypothetical protein (NCBI), at 2,201,994 to 2,202,311 yehK b2119: yehL - orf, hypothetical protein (VIMSS), at 2,202,618 to 2,203,706 yehL Position (kb) 2201 2202 2203Strain fitness (log2 ratio) -1 0 1 2at 2201.038 kb on + strand, within yehIat 2201.043 kb on + strand, within yehIat 2201.050 kb on + strand, within yehIat 2201.050 kb on + strand, within yehIat 2201.217 kb on + strand, within yehIat 2201.225 kb on - strand, within yehIat 2201.225 kb on - strand, within yehIat 2201.259 kb on - strand, within yehIat 2201.300 kb on - strand, within yehIat 2201.304 kb on + strand, within yehIat 2201.360 kb on - strand, within yehIat 2201.360 kb on - strand, within yehIat 2201.371 kb on - strand, within yehIat 2201.411 kb on + strand, within yehIat 2201.443 kb on + strand, within yehIat 2201.613 kb on + strandat 2201.613 kb on + strandat 2201.613 kb on + strandat 2201.645 kb on - strandat 2201.709 kb on + strandat 2201.709 kb on + strandat 2201.746 kb on + strandat 2201.764 kb on - strandat 2201.833 kb on + strandat 2201.833 kb on + strandat 2201.838 kb on + strandat 2201.955 kb on - strandat 2202.021 kb on + strandat 2202.040 kb on + strand, within yehKat 2202.102 kb on + strand, within yehKat 2202.114 kb on + strand, within yehKat 2202.116 kb on + strand, within yehKat 2202.125 kb on + strand, within yehKat 2202.125 kb on + strand, within yehKat 2202.135 kb on + strand, within yehKat 2202.152 kb on + strand, within yehKat 2202.166 kb on + strand, within yehKat 2202.166 kb on + strand, within yehKat 2202.166 kb on + strand, within yehKat 2202.174 kb on - strand, within yehKat 2202.199 kb on + strand, within yehKat 2202.431 kb on + strandat 2202.431 kb on + strandat 2202.437 kb on + strandat 2202.445 kb on + strandat 2202.445 kb on + strandat 2202.655 kb on + strandat 2202.662 kb on + strandat 2202.662 kb on + strandat 2202.663 kb on - strandat 2202.663 kb on - strandat 2202.663 kb on - strandat 2202.663 kb on - strandat 2202.665 kb on - strandat 2202.665 kb on - strandat 2202.665 kb on - strandat 2202.668 kb on - strandat 2202.670 kb on - strandat 2202.675 kb on - strandat 2202.675 kb on - strandat 2202.714 kb on - strandat 2202.719 kb on - strandat 2202.733 kb on - strand, within yehLat 2202.733 kb on - strand, within yehLat 2202.751 kb on + strand, within yehLat 2202.751 kb on + strand, within yehLat 2202.790 kb on + strand, within yehLat 2202.790 kb on + strand, within yehLat 2202.840 kb on - strand, within yehLat 2202.843 kb on - strand, within yehLat 2202.951 kb on + strand, within yehLat 2202.951 kb on + strand, within yehLat 2202.994 kb on - strand, within yehLat 2202.994 kb on - strand, within yehLat 2203.056 kb on - strand, within yehLat 2203.110 kb on + strand, within yehLat 2203.120 kb on + strand, within yehL

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic Allura Red AC 250 uM
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2,201,038 + yehI b2118 0.75 -0.3
2,201,043 + yehI b2118 0.75 +0.2
2,201,050 + yehI b2118 0.76 +0.1
2,201,050 + yehI b2118 0.76 -0.4
2,201,217 + yehI b2118 0.80 -0.8
2,201,225 - yehI b2118 0.80 +0.5
2,201,225 - yehI b2118 0.80 +0.0
2,201,259 - yehI b2118 0.81 +0.3
2,201,300 - yehI b2118 0.83 -0.3
2,201,304 + yehI b2118 0.83 +0.2
2,201,360 - yehI b2118 0.84 +0.5
2,201,360 - yehI b2118 0.84 +0.5
2,201,371 - yehI b2118 0.85 -0.3
2,201,411 + yehI b2118 0.86 +0.1
2,201,443 + yehI b2118 0.86 -0.0
2,201,613 + +0.3
2,201,613 + +0.0
2,201,613 + +0.1
2,201,645 - -1.0
2,201,709 + +0.6
2,201,709 + +0.1
2,201,746 + -0.2
2,201,764 - +0.4
2,201,833 + +0.4
2,201,833 + -0.2
2,201,838 + -0.2
2,201,955 - +2.0
2,202,021 + -0.1
2,202,040 + yehK b4541 0.14 +0.8
2,202,102 + yehK b4541 0.34 -0.2
2,202,114 + yehK b4541 0.38 -0.6
2,202,116 + yehK b4541 0.38 +0.7
2,202,125 + yehK b4541 0.41 +0.5
2,202,125 + yehK b4541 0.41 +0.7
2,202,135 + yehK b4541 0.44 +0.3
2,202,152 + yehK b4541 0.50 +0.0
2,202,166 + yehK b4541 0.54 -0.4
2,202,166 + yehK b4541 0.54 -0.9
2,202,166 + yehK b4541 0.54 +1.5
2,202,174 - yehK b4541 0.57 +1.7
2,202,199 + yehK b4541 0.64 +1.7
2,202,431 + +1.7
2,202,431 + +1.1
2,202,437 + -0.3
2,202,445 + +0.1
2,202,445 + +1.7
2,202,655 + +1.7
2,202,662 + +1.7
2,202,662 + +1.7
2,202,663 - +0.2
2,202,663 - -0.7
2,202,663 - -1.3
2,202,663 - -0.2
2,202,665 - +0.4
2,202,665 - -0.2
2,202,665 - +0.1
2,202,668 - +0.2
2,202,670 - +0.5
2,202,675 - -0.4
2,202,675 - -0.5
2,202,714 - +0.2
2,202,719 - -0.2
2,202,733 - yehL b2119 0.11 -0.4
2,202,733 - yehL b2119 0.11 +1.1
2,202,751 + yehL b2119 0.12 +1.7
2,202,751 + yehL b2119 0.12 +1.7
2,202,790 + yehL b2119 0.16 +1.7
2,202,790 + yehL b2119 0.16 +0.9
2,202,840 - yehL b2119 0.20 +0.7
2,202,843 - yehL b2119 0.21 -0.5
2,202,951 + yehL b2119 0.31 +1.3
2,202,951 + yehL b2119 0.31 -0.3
2,202,994 - yehL b2119 0.35 +0.8
2,202,994 - yehL b2119 0.35 +0.2
2,203,056 - yehL b2119 0.40 -0.1
2,203,110 + yehL b2119 0.45 +0.2
2,203,120 + yehL b2119 0.46 +0.0

Or see this region's nucleotide sequence