Strain Fitness in Escherichia coli BW25113 around b4089
Experiment: LB Anaerobic Allura Red AC 250 uM
Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.
Per-strain Table
Position | Strand | Gene | LocusTag | Fraction | LB Anaerobic Allura Red AC 250 uM |
---|---|---|---|---|---|
remove | |||||
4,309,135 | - | +0.4 | |||
4,309,139 | + | +1.7 | |||
4,309,145 | - | +0.0 | |||
4,309,149 | - | +0.1 | |||
4,309,152 | - | -0.1 | |||
4,309,152 | - | -0.5 | |||
4,309,152 | - | +0.1 | |||
4,309,152 | - | -0.3 | |||
4,309,152 | - | +0.4 | |||
4,309,152 | - | -0.2 | |||
4,309,152 | - | -0.6 | |||
4,309,152 | - | -1.1 | |||
4,309,167 | - | +0.1 | |||
4,309,168 | - | +0.2 | |||
4,309,178 | + | +1.7 | |||
4,309,191 | + | +0.1 | |||
4,309,193 | - | +0.0 | |||
4,309,193 | - | -0.1 | |||
4,309,194 | - | -0.2 | |||
4,309,194 | - | +0.2 | |||
4,309,194 | - | +0.3 | |||
4,309,194 | - | -1.0 | |||
4,309,194 | - | -0.6 | |||
4,309,194 | - | -0.4 | |||
4,309,212 | + | +1.7 | |||
4,309,222 | + | +1.2 | |||
4,309,222 | + | -1.4 | |||
4,309,223 | + | -0.7 | |||
4,309,223 | + | +0.2 | |||
4,309,223 | - | -0.2 | |||
4,309,223 | - | -0.1 | |||
4,309,254 | - | alsB | b4088 | 0.13 | -0.1 |
4,309,254 | - | alsB | b4088 | 0.13 | +0.7 |
4,309,261 | - | alsB | b4088 | 0.14 | -0.2 |
4,309,261 | - | alsB | b4088 | 0.14 | -1.5 |
4,309,261 | - | alsB | b4088 | 0.14 | -0.1 |
4,309,274 | + | alsB | b4088 | 0.15 | -0.4 |
4,309,274 | + | alsB | b4088 | 0.15 | +0.4 |
4,309,287 | - | alsB | b4088 | 0.17 | +0.2 |
4,309,287 | - | alsB | b4088 | 0.17 | +0.5 |
4,309,369 | + | alsB | b4088 | 0.26 | -0.1 |
4,309,369 | + | alsB | b4088 | 0.26 | +0.5 |
4,309,369 | + | alsB | b4088 | 0.26 | -0.1 |
4,309,372 | - | alsB | b4088 | 0.26 | +0.1 |
4,309,372 | - | alsB | b4088 | 0.26 | -0.1 |
4,309,373 | - | alsB | b4088 | 0.26 | -0.3 |
4,309,378 | - | alsB | b4088 | 0.26 | +0.1 |
4,309,435 | - | alsB | b4088 | 0.33 | +0.2 |
4,309,524 | + | alsB | b4088 | 0.42 | +0.9 |
4,309,524 | + | alsB | b4088 | 0.42 | +0.6 |
4,309,531 | - | alsB | b4088 | 0.43 | -0.4 |
4,309,603 | - | alsB | b4088 | 0.51 | +0.2 |
4,309,635 | + | alsB | b4088 | 0.54 | +0.0 |
4,309,642 | + | alsB | b4088 | 0.55 | -0.2 |
4,309,647 | + | alsB | b4088 | 0.55 | +0.1 |
4,309,647 | + | alsB | b4088 | 0.55 | +0.1 |
4,309,654 | + | alsB | b4088 | 0.56 | -0.3 |
4,309,654 | + | alsB | b4088 | 0.56 | +0.1 |
4,309,662 | + | alsB | b4088 | 0.57 | -1.0 |
4,309,710 | - | alsB | b4088 | 0.62 | -0.1 |
4,309,750 | + | alsB | b4088 | 0.66 | -0.3 |
4,309,758 | + | alsB | b4088 | 0.67 | +0.0 |
4,309,760 | + | alsB | b4088 | 0.67 | +0.5 |
4,309,763 | + | alsB | b4088 | 0.68 | -0.0 |
4,309,763 | + | alsB | b4088 | 0.68 | +0.4 |
4,309,770 | + | alsB | b4088 | 0.68 | +0.2 |
4,309,770 | + | alsB | b4088 | 0.68 | +0.4 |
4,309,780 | + | alsB | b4088 | 0.69 | +0.6 |
4,309,780 | + | alsB | b4088 | 0.69 | +0.0 |
4,309,780 | + | alsB | b4088 | 0.69 | +0.2 |
4,309,783 | + | alsB | b4088 | 0.70 | +0.1 |
4,309,790 | + | alsB | b4088 | 0.71 | -0.4 |
4,309,790 | + | alsB | b4088 | 0.71 | +0.6 |
4,309,790 | + | alsB | b4088 | 0.71 | -0.8 |
4,309,790 | + | alsB | b4088 | 0.71 | -0.6 |
4,309,790 | + | alsB | b4088 | 0.71 | -0.2 |
4,309,790 | + | alsB | b4088 | 0.71 | -0.6 |
4,309,795 | + | alsB | b4088 | 0.71 | -0.6 |
4,309,795 | + | alsB | b4088 | 0.71 | +0.1 |
4,309,805 | + | alsB | b4088 | 0.72 | -0.0 |
4,309,847 | + | alsB | b4088 | 0.77 | -0.9 |
4,309,847 | + | alsB | b4088 | 0.77 | -0.9 |
4,309,847 | + | alsB | b4088 | 0.77 | -0.5 |
4,309,871 | + | alsB | b4088 | 0.79 | -0.8 |
4,309,873 | + | alsB | b4088 | 0.79 | +0.3 |
4,309,873 | + | alsB | b4088 | 0.79 | -0.2 |
4,309,881 | - | alsB | b4088 | 0.80 | +1.7 |
4,309,937 | + | alsB | b4088 | 0.86 | -0.0 |
4,309,973 | + | +0.0 | |||
4,309,983 | + | -0.2 | |||
4,309,988 | - | +0.4 | |||
4,309,988 | - | +0.7 | |||
4,310,004 | + | -0.7 | |||
4,310,004 | + | +0.2 | |||
4,310,009 | + | +0.2 | |||
4,310,010 | + | +0.4 | |||
4,310,017 | + | +0.5 | |||
4,310,018 | + | -0.0 | |||
4,310,024 | + | +0.1 | |||
4,310,024 | + | +1.6 | |||
4,310,024 | + | -0.5 | |||
4,310,024 | + | -0.2 | |||
4,310,024 | + | -0.2 | |||
4,310,073 | + | +2.7 | |||
4,310,081 | + | +0.3 | |||
4,310,082 | - | +2.0 | |||
4,310,082 | - | -0.6 | |||
4,310,087 | - | -1.0 | |||
4,310,087 | - | +1.4 | |||
4,310,091 | - | -1.5 | |||
4,310,095 | - | -0.3 | |||
4,310,097 | + | +1.7 | |||
4,310,099 | - | -0.6 | |||
4,310,099 | - | +1.4 | |||
4,310,099 | - | +0.3 | |||
4,310,127 | - | -1.5 | |||
4,310,127 | - | -1.0 | |||
4,310,148 | - | -0.4 | |||
4,310,148 | - | -0.2 | |||
4,310,148 | - | +0.1 | |||
4,310,159 | - | +0.9 | |||
4,310,159 | - | -0.8 | |||
4,310,190 | - | -0.2 | |||
4,310,190 | - | +1.0 | |||
4,310,190 | - | +0.4 | |||
4,310,190 | - | +1.0 | |||
4,310,200 | - | +0.0 | |||
4,310,205 | - | +1.5 | |||
4,310,205 | - | +0.4 | |||
4,310,215 | - | rpiR | b4089 | 0.10 | -0.0 |
4,310,215 | - | rpiR | b4089 | 0.10 | +0.5 |
4,310,215 | - | rpiR | b4089 | 0.10 | +0.3 |
4,310,215 | - | rpiR | b4089 | 0.10 | -0.2 |
4,310,215 | - | rpiR | b4089 | 0.10 | -0.5 |
4,310,217 | - | rpiR | b4089 | 0.10 | -0.1 |
4,310,269 | - | rpiR | b4089 | 0.16 | +0.3 |
4,310,282 | - | rpiR | b4089 | 0.18 | +1.1 |
4,310,294 | - | rpiR | b4089 | 0.19 | +0.8 |
4,310,294 | - | rpiR | b4089 | 0.19 | +1.1 |
4,310,294 | - | rpiR | b4089 | 0.19 | +0.8 |
4,310,294 | - | rpiR | b4089 | 0.19 | -0.1 |
4,310,314 | - | rpiR | b4089 | 0.21 | +0.8 |
4,310,329 | - | rpiR | b4089 | 0.23 | +0.8 |
4,310,329 | - | rpiR | b4089 | 0.23 | +0.5 |
4,310,335 | + | rpiR | b4089 | 0.24 | +2.7 |
4,310,335 | + | rpiR | b4089 | 0.24 | +0.7 |
4,310,337 | + | rpiR | b4089 | 0.24 | +1.7 |
4,310,341 | - | rpiR | b4089 | 0.24 | +0.1 |
4,310,341 | - | rpiR | b4089 | 0.24 | +1.0 |
4,310,343 | + | rpiR | b4089 | 0.25 | +1.7 |
4,310,350 | - | rpiR | b4089 | 0.25 | -0.0 |
4,310,351 | - | rpiR | b4089 | 0.25 | +0.2 |
4,310,351 | - | rpiR | b4089 | 0.25 | +0.1 |
4,310,351 | - | rpiR | b4089 | 0.25 | +0.5 |
4,310,356 | - | rpiR | b4089 | 0.26 | +0.6 |
4,310,364 | - | rpiR | b4089 | 0.27 | +0.5 |
4,310,387 | + | rpiR | b4089 | 0.30 | +0.7 |
4,310,395 | - | rpiR | b4089 | 0.30 | +0.0 |
4,310,395 | - | rpiR | b4089 | 0.30 | +1.3 |
4,310,396 | - | rpiR | b4089 | 0.31 | +0.1 |
4,310,399 | + | rpiR | b4089 | 0.31 | -1.1 |
4,310,407 | - | rpiR | b4089 | 0.32 | +1.4 |
4,310,407 | - | rpiR | b4089 | 0.32 | +0.6 |
4,310,415 | - | rpiR | b4089 | 0.33 | +0.3 |
4,310,415 | - | rpiR | b4089 | 0.33 | +1.3 |
4,310,415 | - | rpiR | b4089 | 0.33 | +0.3 |
4,310,420 | - | rpiR | b4089 | 0.33 | +0.7 |
4,310,425 | - | rpiR | b4089 | 0.34 | +1.1 |
4,310,425 | - | rpiR | b4089 | 0.34 | +0.5 |
4,310,431 | + | rpiR | b4089 | 0.34 | -1.6 |
4,310,432 | + | rpiR | b4089 | 0.35 | -0.1 |
4,310,432 | + | rpiR | b4089 | 0.35 | -0.3 |
4,310,432 | + | rpiR | b4089 | 0.35 | +0.6 |
4,310,432 | + | rpiR | b4089 | 0.35 | +0.7 |
4,310,433 | + | rpiR | b4089 | 0.35 | +0.3 |
4,310,433 | + | rpiR | b4089 | 0.35 | +0.1 |
4,310,438 | + | rpiR | b4089 | 0.35 | +0.5 |
4,310,441 | - | rpiR | b4089 | 0.36 | +0.7 |
4,310,441 | - | rpiR | b4089 | 0.36 | +0.8 |
4,310,442 | - | rpiR | b4089 | 0.36 | +0.3 |
4,310,454 | - | rpiR | b4089 | 0.37 | +0.9 |
4,310,456 | - | rpiR | b4089 | 0.37 | +1.4 |
4,310,475 | + | rpiR | b4089 | 0.39 | -0.5 |
4,310,475 | + | rpiR | b4089 | 0.39 | -0.4 |
4,310,480 | - | rpiR | b4089 | 0.40 | +0.0 |
4,310,484 | - | rpiR | b4089 | 0.40 | +1.0 |
4,310,484 | - | +0.3 | |||
4,310,484 | - | rpiR | b4089 | 0.40 | +0.2 |
4,310,484 | - | rpiR | b4089 | 0.40 | +0.4 |
4,310,487 | + | rpiR | b4089 | 0.41 | -0.1 |
4,310,489 | + | rpiR | b4089 | 0.41 | -0.4 |
4,310,489 | + | rpiR | b4089 | 0.41 | +0.3 |
4,310,494 | + | rpiR | b4089 | 0.42 | -0.3 |
4,310,494 | - | rpiR | b4089 | 0.42 | +0.5 |
4,310,494 | - | rpiR | b4089 | 0.42 | +0.4 |
4,310,499 | + | rpiR | b4089 | 0.42 | +0.0 |
4,310,499 | + | rpiR | b4089 | 0.42 | +0.0 |
4,310,508 | + | rpiR | b4089 | 0.43 | +0.1 |
4,310,508 | + | rpiR | b4089 | 0.43 | -0.2 |
4,310,553 | - | rpiR | b4089 | 0.48 | +0.4 |
4,310,564 | - | rpiR | b4089 | 0.49 | +0.2 |
4,310,564 | - | rpiR | b4089 | 0.49 | -0.7 |
4,310,572 | - | rpiR | b4089 | 0.50 | +0.9 |
4,310,596 | - | rpiR | b4089 | 0.53 | +0.3 |
4,310,706 | - | rpiR | b4089 | 0.65 | -0.5 |
4,310,739 | - | rpiR | b4089 | 0.69 | -1.0 |
4,310,739 | - | rpiR | b4089 | 0.69 | -1.2 |
4,310,789 | - | rpiR | b4089 | 0.75 | +0.8 |
4,310,789 | - | rpiR | b4089 | 0.75 | +0.1 |
4,310,869 | - | rpiR | b4089 | 0.84 | +0.7 |
4,310,880 | - | rpiR | b4089 | 0.85 | -0.2 |
4,310,886 | - | rpiR | b4089 | 0.86 | +0.5 |
4,310,886 | - | rpiR | b4089 | 0.86 | -0.3 |
4,310,919 | - | rpiR | b4089 | 0.89 | +0.4 |
4,310,919 | - | rpiR | b4089 | 0.89 | +0.1 |
4,310,943 | - | +0.9 | |||
4,310,969 | + | +0.2 | |||
4,310,969 | + | -0.5 | |||
4,310,969 | + | +0.0 | |||
4,310,969 | + | +0.4 | |||
4,310,971 | - | +0.0 | |||
4,310,977 | - | -1.5 | |||
4,310,977 | - | +0.1 | |||
4,310,977 | - | -0.9 | |||
4,310,977 | - | -0.3 | |||
4,310,978 | - | +0.3 | |||
4,310,992 | + | -0.7 | |||
4,311,021 | + | +0.4 | |||
4,311,052 | - | +0.2 | |||
4,311,056 | - | -0.1 | |||
4,311,242 | + | +0.7 | |||
4,311,242 | + | -0.6 | |||
4,311,499 | - | rpiB | b4090 | 0.28 | +0.5 |
4,311,629 | + | rpiB | b4090 | 0.57 | +0.3 |
4,311,639 | + | rpiB | b4090 | 0.59 | -0.2 |
4,311,639 | + | rpiB | b4090 | 0.59 | +0.2 |
4,311,674 | - | rpiB | b4090 | 0.67 | +0.0 |
4,311,710 | + | rpiB | b4090 | 0.75 | -0.3 |
4,311,744 | + | rpiB | b4090 | 0.82 | -0.4 |
4,311,744 | + | rpiB | b4090 | 0.82 | -0.0 |
4,311,744 | + | rpiB | b4090 | 0.82 | -0.0 |
4,311,754 | + | rpiB | b4090 | 0.85 | +0.2 |
4,311,756 | + | rpiB | b4090 | 0.85 | -0.2 |
4,311,756 | + | rpiB | b4090 | 0.85 | -0.6 |
4,311,756 | + | rpiB | b4090 | 0.85 | -0.0 |
4,311,797 | + | -0.5 | |||
4,311,852 | - | +0.2 | |||
4,311,852 | - | -1.5 | |||
4,311,889 | - | +0.4 | |||
4,311,933 | - | yjdP | b4487 | 0.13 | +0.3 |
4,311,933 | - | yjdP | b4487 | 0.13 | -0.2 |
4,311,943 | - | yjdP | b4487 | 0.16 | -0.1 |
4,311,969 | - | yjdP | b4487 | 0.24 | -0.6 |
4,311,969 | - | yjdP | b4487 | 0.24 | +0.6 |
4,311,983 | - | yjdP | b4487 | 0.28 | -0.5 |
4,311,983 | - | yjdP | b4487 | 0.28 | -0.7 |
4,312,006 | + | yjdP | b4487 | 0.35 | -0.7 |
4,312,006 | + | yjdP | b4487 | 0.35 | -0.4 |
4,312,006 | + | yjdP | b4487 | 0.35 | +0.4 |
Or see this region's nucleotide sequence