Strain Fitness in Escherichia coli BW25113 around b2278

Experiment: LB Anaerobic Allura Red AC 250 uM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	LB Anaerobic Allura Red AC 250 uM
2,390,230	-	nuoM	b2277	0.45	+0.5
2,390,312	+	nuoM	b2277	0.51	+1.7
2,390,341	+	nuoM	b2277	0.53	+1.7
2,390,383	-	nuoM	b2277	0.55	+0.1
2,390,411	+	nuoM	b2277	0.57	+0.6
2,390,426	-	nuoM	b2277	0.58	+0.7
2,390,454	-	nuoM	b2277	0.60	+0.5
2,390,511	-	nuoM	b2277	0.64	+1.3
2,390,516	-	nuoM	b2277	0.64	+1.1
2,390,516	-	nuoM	b2277	0.64	+1.0
2,390,522	-				+0.1
2,390,522	-	nuoM	b2277	0.65	+0.4
2,390,530	+	nuoM	b2277	0.65	+0.7
2,390,575	-	nuoM	b2277	0.68	+0.2
2,390,591	-	nuoM	b2277	0.69	-0.2
2,390,591	-	nuoM	b2277	0.69	+1.6
2,390,596	-	nuoM	b2277	0.69	+1.3
2,390,598	-	nuoM	b2277	0.70	+0.0
2,390,886	-	nuoM	b2277	0.88	-0.8
2,390,886	-	nuoM	b2277	0.88	+0.1
2,390,912	-				-1.3
2,390,948	+				-0.8
2,390,972	+				+0.5
2,391,053	+				+0.4
2,391,068	-				-0.3
2,391,102	+				+0.3
2,391,150	+				+0.9
2,391,153	+				+0.1
2,391,197	+				+1.0
2,391,238	-				-1.9
2,391,245	-				-1.2
2,391,248	+				+0.8
2,391,259	+				+0.2
2,391,278	-				+0.4
2,391,281	-				+1.7
2,391,281	-				-0.9
2,391,317	+				+0.1
2,391,372	-				-1.1
2,391,414	+				+1.1
2,391,414	+	nuoL	b2278	0.10	+0.1
2,391,419	+	nuoL	b2278	0.10	-0.5
2,391,439	-	nuoL	b2278	0.12	-1.6
2,391,505	-	nuoL	b2278	0.15	-1.8
2,391,505	-	nuoL	b2278	0.15	+0.7
2,391,517	+	nuoL	b2278	0.16	+1.0
2,391,567	-	nuoL	b2278	0.18	-0.1
2,391,785	+	nuoL	b2278	0.30	-0.2
2,391,795	+	nuoL	b2278	0.31	+1.3
2,391,809	+	nuoL	b2278	0.32	-0.4
2,391,809	+	nuoL	b2278	0.32	+0.5
2,391,921	-	nuoL	b2278	0.38	+1.2
2,391,985	-	nuoL	b2278	0.41	+1.0
2,392,049	-	nuoL	b2278	0.45	+0.1
2,392,091	-	nuoL	b2278	0.47	-0.9
2,392,137	+	nuoL	b2278	0.49	+0.3
2,392,139	-	nuoL	b2278	0.50	+0.4
2,392,139	-	nuoL	b2278	0.50	-1.5
2,392,161	-	nuoL	b2278	0.51	-1.9
2,392,164	+	nuoL	b2278	0.51	-1.2
2,392,164	+	nuoL	b2278	0.51	-0.1
2,392,168	+	nuoL	b2278	0.51	+0.4
2,392,192	+	nuoL	b2278	0.52	+0.4
2,392,206	-	nuoL	b2278	0.53	-1.6
2,392,226	+	nuoL	b2278	0.54	-0.8
2,392,234	+	nuoL	b2278	0.55	+0.4
2,392,245	+	nuoL	b2278	0.55	+1.5
2,392,245	+	nuoL	b2278	0.55	+0.7
2,392,245	+				-0.4
2,392,253	-	nuoL	b2278	0.56	+0.9
2,392,253	-	nuoL	b2278	0.56	-0.2
2,392,255	+	nuoL	b2278	0.56	+0.7
2,392,290	-	nuoL	b2278	0.58	-0.2
2,392,315	+	nuoL	b2278	0.59	+0.7
2,392,403	-	nuoL	b2278	0.64	+0.5
2,392,403	-	nuoL	b2278	0.64	-0.2
2,392,419	-	nuoL	b2278	0.65	+0.4
2,392,424	-	nuoL	b2278	0.65	+1.5
2,392,488	-	nuoL	b2278	0.68	+0.0
2,392,498	-	nuoL	b2278	0.69	-0.0
2,392,537	-	nuoL	b2278	0.71	+0.7
2,392,555	-	nuoL	b2278	0.72	+1.2
2,392,578	+	nuoL	b2278	0.73	+1.6
2,392,593	+	nuoL	b2278	0.74	+1.1
2,392,593	+	nuoL	b2278	0.74	+2.1
2,392,593	+	nuoL	b2278	0.74	+0.4
2,392,601	-	nuoL	b2278	0.75	+1.3
2,392,601	-	nuoL	b2278	0.75	+0.4
2,392,601	-	nuoL	b2278	0.75	-0.6
2,392,620	+	nuoL	b2278	0.76	+0.1
2,392,627	+	nuoL	b2278	0.76	-0.2
2,392,694	-	nuoL	b2278	0.80	+0.4
2,392,723	+	nuoL	b2278	0.81	-1.1
2,392,723	+	nuoL	b2278	0.81	+1.2
2,392,728	+	nuoL	b2278	0.81	+2.4
2,392,728	+	nuoL	b2278	0.81	+0.3
2,392,771	+	nuoL	b2278	0.84	+0.9
2,392,776	+	nuoL	b2278	0.84	+1.0
2,392,831	-	nuoL	b2278	0.87	-0.5
2,392,831	-	nuoL	b2278	0.87	-0.4
2,392,831	-	nuoL	b2278	0.87	+0.1
2,392,831	-	nuoL	b2278	0.87	+1.2
2,392,874	-	nuoL	b2278	0.89	+0.2
2,392,895	-				-0.2
2,393,077	+				+2.2
2,393,077	+				-0.4
2,393,106	+	nuoK	b2279	0.14	+0.7
2,393,152	-	nuoK	b2279	0.29	-1.8
2,393,213	-	nuoK	b2279	0.49	+1.1
2,393,323	+	nuoK	b2279	0.85	+0.7
2,393,346	+				-0.4
2,393,410	+				+0.6
2,393,412	+				+0.1
2,393,412	+				+0.2
2,393,419	+				+2.0
2,393,456	-	nuoJ	b2280	0.17	+0.1
2,393,463	-	nuoJ	b2280	0.18	+0.0
2,393,530	-	nuoJ	b2280	0.30	+0.6
2,393,530	-	nuoJ	b2280	0.30	+0.7
2,393,536	+	nuoJ	b2280	0.31	+0.1
2,393,559	+	nuoJ	b2280	0.35	+1.5
2,393,581	-	nuoJ	b2280	0.39	+0.1
2,393,586	-	nuoJ	b2280	0.40	+0.7
2,393,606	+	nuoJ	b2280	0.44	+1.4
2,393,611	-	nuoJ	b2280	0.45	+0.6
2,393,617	+	nuoJ	b2280	0.46	+0.5
2,393,637	+	nuoJ	b2280	0.49	-0.7
2,393,638	+	nuoJ	b2280	0.49	-0.1
2,393,638	+	nuoJ	b2280	0.49	+1.3
2,393,645	+	nuoJ	b2280	0.51	+0.9
2,393,724	+	nuoJ	b2280	0.65	+1.1
2,393,731	-	nuoJ	b2280	0.66	+0.8
2,393,839	-	nuoJ	b2280	0.86	-0.7
2,393,840	+	nuoJ	b2280	0.86	-0.3
2,393,876	+				+0.7
2,393,898	-				-0.4
2,393,915	+				-0.9
2,393,915	+				-0.3
2,393,967	-				+0.2
2,393,967	-				-0.5
2,394,043	+	nuoI	b2281	0.21	-0.5