Experiment: LB Anaerobic Allura Red AC 250 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yaeG and yaeH are separated by 88 nucleotides yaeH and yaeI are separated by 161 nucleotides yaeI and dapD are separated by 53 nucleotides dapD and glnD are separated by 30 nucleotides
b0162: yaeG - orf, hypothetical protein (VIMSS), at 182,463 to 183,620
yaeG
b0163: yaeH - hypothetical protein (NCBI), at 183,709 to 184,095
yaeH
b0164: yaeI - orf, hypothetical protein (VIMSS), at 184,257 to 185,069
yaeI
b0166: dapD - 2,3,4,5-tetrahydropyridine-2-carboxylate N-succinyltransferase (NCBI), at 185,123 to 185,947
dapD
b0167: glnD - PII uridylyl-transferase (NCBI), at 185,978 to 188,650
glnD
Position (kb) 184
185
186 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 183.275 kb on + strand, within yaeG at 183.337 kb on - strand, within yaeG at 183.510 kb on + strand at 183.518 kb on - strand at 183.518 kb on - strand at 183.543 kb on - strand at 183.584 kb on + strand at 183.584 kb on + strand at 183.643 kb on + strand at 183.663 kb on + strand at 183.663 kb on + strand at 183.672 kb on + strand at 183.904 kb on - strand, within yaeH at 183.924 kb on - strand, within yaeH at 183.929 kb on + strand, within yaeH at 183.937 kb on - strand, within yaeH at 183.943 kb on + strand, within yaeH at 184.035 kb on + strand, within yaeH at 184.100 kb on - strand at 184.130 kb on + strand at 184.193 kb on - strand at 184.283 kb on - strand at 184.283 kb on - strand at 184.312 kb on + strand at 184.351 kb on - strand, within yaeI at 184.439 kb on + strand, within yaeI at 184.455 kb on + strand, within yaeI at 184.579 kb on + strand, within yaeI at 184.622 kb on - strand, within yaeI at 184.636 kb on - strand, within yaeI at 184.636 kb on - strand, within yaeI at 184.701 kb on - strand, within yaeI at 184.755 kb on + strand, within yaeI at 184.757 kb on - strand, within yaeI at 184.899 kb on - strand, within yaeI at 184.899 kb on - strand, within yaeI at 184.928 kb on + strand, within yaeI at 184.928 kb on + strand, within yaeI at 184.993 kb on - strand at 184.993 kb on - strand at 185.102 kb on + strand at 185.102 kb on + strand at 185.105 kb on - strand at 185.107 kb on + strand at 185.949 kb on + strand at 185.969 kb on + strand at 185.969 kb on + strand at 185.976 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic Allura Red AC 250 uM remove 183,275 + yaeG b0162 0.70 -0.0 183,337 - yaeG b0162 0.75 +0.0 183,510 + -0.3 183,518 - +0.3 183,518 - +0.9 183,543 - -0.1 183,584 + +0.0 183,584 + -0.2 183,643 + -0.3 183,663 + -0.5 183,663 + -0.4 183,672 + -0.1 183,904 - yaeH b0163 0.50 -0.1 183,924 - yaeH b0163 0.56 -0.4 183,929 + yaeH b0163 0.57 -1.0 183,937 - yaeH b0163 0.59 +0.4 183,943 + yaeH b0163 0.60 -0.0 184,035 + yaeH b0163 0.84 +0.7 184,100 - +0.3 184,130 + -3.8 184,193 - +0.3 184,283 - +0.3 184,283 - -0.2 184,312 + -0.7 184,351 - yaeI b0164 0.12 -1.0 184,439 + yaeI b0164 0.22 +0.8 184,455 + yaeI b0164 0.24 -0.7 184,579 + yaeI b0164 0.40 +0.1 184,622 - yaeI b0164 0.45 +0.5 184,636 - yaeI b0164 0.47 +0.2 184,636 - yaeI b0164 0.47 -0.3 184,701 - yaeI b0164 0.55 +0.1 184,755 + yaeI b0164 0.61 +0.2 184,757 - yaeI b0164 0.62 +0.3 184,899 - yaeI b0164 0.79 +0.4 184,899 - yaeI b0164 0.79 +1.2 184,928 + yaeI b0164 0.83 +0.4 184,928 + yaeI b0164 0.83 +0.6 184,993 - -0.1 184,993 - -0.3 185,102 + +0.0 185,102 + +0.3 185,105 - -0.1 185,107 + -0.9 185,949 + +0.1 185,969 + -0.3 185,969 + +0.1 185,976 - -0.5
Or see this region's nucleotide sequence