Experiment: LB Anaerobic Allura Red AC 250 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycgF and ycgZ are separated by 313 nucleotides ycgZ and ymgA are separated by 42 nucleotides ymgA and ymgB are separated by 28 nucleotides ymgB and ymgC are separated by 112 nucleotides ymgC and b1168 are separated by 331 nucleotides
b1163: ycgF - predicted FAD-binding phosphodiesterase (NCBI), at 1,213,487 to 1,214,698
ycgF
b1164: ycgZ - hypothetical protein (NCBI), at 1,215,012 to 1,215,248
ycgZ
b1165: ymgA - hypothetical protein (NCBI), at 1,215,291 to 1,215,563
ymgA
b1166: ymgB - hypothetical protein (NCBI), at 1,215,592 to 1,215,858
ymgB
b1167: ymgC - hypothetical protein (NCBI), at 1,215,971 to 1,216,219
ymgC
b1168: b1168 - putative proteases (VIMSS), at 1,216,551 to 1,218,074
b1168
Position (kb)
1215
1216 Strain fitness (log2 ratio)
-2
-1
0
1 at 1214.366 kb on + strand, within ycgF at 1214.460 kb on + strand, within ycgF at 1214.460 kb on - strand, within ycgF at 1214.478 kb on + strand, within ycgF at 1214.541 kb on - strand, within ycgF at 1214.575 kb on + strand, within ycgF at 1214.590 kb on - strand at 1214.596 kb on - strand at 1214.612 kb on - strand at 1214.614 kb on + strand at 1214.614 kb on + strand at 1214.617 kb on - strand at 1214.718 kb on - strand at 1214.718 kb on - strand at 1214.730 kb on - strand at 1214.730 kb on - strand at 1214.730 kb on - strand at 1214.789 kb on + strand at 1215.133 kb on + strand, within ycgZ at 1215.188 kb on + strand, within ycgZ at 1215.190 kb on + strand, within ycgZ at 1215.288 kb on + strand at 1215.346 kb on + strand, within ymgA at 1215.347 kb on + strand, within ymgA at 1215.349 kb on + strand, within ymgA at 1216.034 kb on + strand, within ymgC at 1216.162 kb on + strand, within ymgC at 1216.213 kb on + strand at 1216.243 kb on + strand at 1216.274 kb on - strand at 1216.358 kb on - strand at 1216.474 kb on - strand at 1216.477 kb on - strand at 1216.479 kb on - strand at 1216.480 kb on + strand at 1216.481 kb on - strand at 1216.491 kb on - strand at 1216.491 kb on - strand at 1216.493 kb on - strand at 1216.517 kb on + strand at 1216.563 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic Allura Red AC 250 uM remove 1,214,366 + ycgF b1163 0.73 -1.2 1,214,460 + ycgF b1163 0.80 +1.1 1,214,460 - ycgF b1163 0.80 +1.6 1,214,478 + ycgF b1163 0.82 +1.6 1,214,541 - ycgF b1163 0.87 +0.1 1,214,575 + ycgF b1163 0.90 +0.6 1,214,590 - +0.6 1,214,596 - +0.9 1,214,612 - -0.8 1,214,614 + +0.3 1,214,614 + -2.3 1,214,617 - +0.4 1,214,718 - -0.6 1,214,718 - +0.6 1,214,730 - +1.1 1,214,730 - -0.2 1,214,730 - +0.3 1,214,789 + +1.6 1,215,133 + ycgZ b1164 0.51 -1.2 1,215,188 + ycgZ b1164 0.74 -0.4 1,215,190 + ycgZ b1164 0.75 +0.1 1,215,288 + +1.6 1,215,346 + ymgA b1165 0.20 +1.6 1,215,347 + ymgA b1165 0.21 +1.6 1,215,349 + ymgA b1165 0.21 +1.6 1,216,034 + ymgC b1167 0.25 +1.6 1,216,162 + ymgC b1167 0.77 +1.6 1,216,213 + +1.6 1,216,243 + +0.6 1,216,274 - -0.0 1,216,358 - +0.6 1,216,474 - -0.5 1,216,477 - +0.0 1,216,479 - -1.0 1,216,480 + +1.6 1,216,481 - +0.3 1,216,491 - +0.6 1,216,491 - +0.6 1,216,493 - -0.2 1,216,517 + +0.4 1,216,563 - +0.1
Or see this region's nucleotide sequence