Strain Fitness in Escherichia coli BW25113 around b1156

Experiment: LB Anaerobic Allura Red AC 250 uM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	LB Anaerobic Allura Red AC 250 uM
1,207,069	+	ycfK	b1154	0.55	-0.4
1,207,070	+	ycfK	b1154	0.55	-0.2
1,207,076	+	ycfK	b1154	0.56	-0.3
1,207,084	-	ycfK	b1154	0.57	-0.1
1,207,097	+	ycfK	b1154	0.59	-1.9
1,207,147	+	ycfK	b1154	0.67	+0.6
1,207,155	+	ycfK	b1154	0.68	-1.0
1,207,156	+	ycfK	b1154	0.69	-0.7
1,207,164	+	ycfK	b1154	0.70	+0.3
1,207,169	+	ycfK	b1154	0.71	+0.6
1,207,177	+	ycfK	b1154	0.72	-0.3
1,207,177	+	ycfK	b1154	0.72	-0.1
1,207,192	+	ycfK	b1154	0.74	-0.2
1,207,246	+	ycfK	b1154	0.83	+0.5
1,207,248	+	ycfK	b1154	0.83	-0.3
1,207,248	+	ycfK	b1154	0.83	+0.7
1,207,258	+	ycfK	b1154	0.85	+0.2
1,207,258	+	ycfK	b1154	0.85	-0.3
1,207,260	+	ycfK	b1154	0.85	+0.2
1,207,267	+	ycfK	b1154	0.86	+0.3
1,207,270	+	ycfK	b1154	0.87	-0.5
1,207,291	+				-0.9
1,207,291	+				-0.0
1,207,339	+				+0.7
1,207,390	+				-0.1
1,207,390	+				-0.1
1,207,540	-	ymfS	b1155	0.45	-1.2
1,207,545	-	ymfS	b1155	0.46	+0.2
1,207,563	+	ymfS	b1155	0.50	+1.3
1,207,605	+	ymfS	b1155	0.60	-0.0
1,207,617	+	ymfS	b1155	0.63	-0.8
1,207,617	+	ymfS	b1155	0.63	+0.5
1,207,624	-	ymfS	b1155	0.65	+0.1
1,207,624	-	ymfS	b1155	0.65	-1.5
1,207,626	+	ymfS	b1155	0.65	+0.5
1,207,633	+	ymfS	b1155	0.67	-0.2
1,207,649	-	ymfS	b1155	0.71	-0.0
1,207,681	+	ymfS	b1155	0.79	+0.0
1,207,703	-	ymfS	b1155	0.84	+1.6
1,207,746	-				-0.1
1,207,746	-				-0.8
1,207,746	-				+0.3
1,207,746	-				+0.4
1,207,772	-				+0.3
1,207,780	+				+1.4
1,207,780	+				-0.4
1,207,792	+				-0.0
1,207,803	-	tfaE	b1156	0.10	+1.5
1,207,815	+	tfaE	b1156	0.12	-0.8
1,207,815	+	tfaE	b1156	0.12	+0.7
1,207,859	-	tfaE	b1156	0.20	-0.0
1,207,959	+	tfaE	b1156	0.36	-0.4
1,207,962	-	tfaE	b1156	0.37	+0.1
1,207,964	-	tfaE	b1156	0.37	+0.1
1,207,967	+	tfaE	b1156	0.38	-0.6
1,207,972	+	tfaE	b1156	0.38	-0.4
1,208,100	-	tfaE	b1156	0.60	+1.6
1,208,167	-	tfaE	b1156	0.71	+0.6
1,208,167	-	tfaE	b1156	0.71	+1.8
1,208,168	-	tfaE	b1156	0.71	+0.5
1,208,168	-	tfaE	b1156	0.71	+0.4
1,208,170	+	tfaE	b1156	0.71	+1.6
1,208,170	+	tfaE	b1156	0.71	-0.0
1,208,172	+	tfaE	b1156	0.72	+1.6
1,208,177	+	tfaE	b1156	0.72	+1.6
1,208,178	-	tfaE	b1156	0.73	-0.2
1,208,178	-	tfaE	b1156	0.73	-0.8
1,208,180	-	tfaE	b1156	0.73	+1.6
1,208,180	-	tfaE	b1156	0.73	+0.8
1,208,184	+	tfaE	b1156	0.74	+1.6
1,208,185	-	tfaE	b1156	0.74	+0.3
1,208,187	-	tfaE	b1156	0.74	+1.9
1,208,194	+	tfaE	b1156	0.75	+0.6
1,208,194	+	tfaE	b1156	0.75	+1.6
1,208,194	+	tfaE	b1156	0.75	+1.6
1,208,352	-				-0.8
1,208,413	-	stfE	b1157	0.14	+0.6
1,208,413	-	stfE	b1157	0.14	-1.2
1,208,441	-	stfE	b1157	0.20	+0.1
1,208,560	-	stfE	b1157	0.44	+0.1
1,208,560	-	stfE	b1157	0.44	-0.2
1,208,575	-	stfE	b1157	0.47	-0.6
1,208,576	-	stfE	b1157	0.47	-0.3
1,208,586	-	stfE	b1157	0.49	-0.1
1,208,604	-	stfE	b1157	0.52	-0.4
1,208,604	-	stfE	b1157	0.52	+2.0
1,208,604	-	stfE	b1157	0.52	-0.5
1,208,606	-	stfE	b1157	0.53	+0.6
1,208,609	-	stfE	b1157	0.53	+0.0
1,208,638	-	stfE	b1157	0.59	+0.2
1,208,642	-	stfE	b1157	0.60	+0.6
1,208,642	-	stfE	b1157	0.60	-0.1
1,208,642	-	stfE	b1157	0.60	+0.1
1,208,648	+	stfE	b1157	0.61	-0.8
1,208,752	+	stfE	b1157	0.82	+0.3
1,208,756	-	stfE	b1157	0.83	+0.1
1,208,791	-	stfE	b1157	0.90	+0.9
1,208,819	+				+0.1
1,208,838	-				+0.2
1,208,899	-				+0.4
1,208,946	+				-1.3
1,208,946	+				-1.1
1,208,964	-	pin	b1158	0.10	+0.8
1,208,964	-	pin	b1158	0.10	+0.4
1,208,968	+	pin	b1158	0.11	+0.1
1,209,015	-	pin	b1158	0.19	+0.0
1,209,110	-	pin	b1158	0.36	+1.6
1,209,296	+	pin	b1158	0.70	-0.7
1,209,296	+	pin	b1158	0.70	-0.1
1,209,306	+	pin	b1158	0.72	+0.0
1,209,306	+				+1.4
1,209,316	+	pin	b1158	0.74	-0.2
1,209,316	+	pin	b1158	0.74	+0.1
1,209,333	+	pin	b1158	0.77	+0.1