Strain Fitness in Escherichia coli BW25113 around b4097

Experiment: LB Anaerobic Sulfasalazine 250 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntphnM and phnL overlap by 4 nucleotidesphnL and phnK are separated by 110 nucleotidesphnK and phnJ overlap by 4 nucleotidesphnJ and phnI overlap by 8 nucleotides b4095: phnM - carbon-phosphorus lyase complex subunit (NCBI), at 4,314,105 to 4,315,241 phnM b4096: phnL - carbon-phosphorus lyase complex subunit (NCBI), at 4,315,238 to 4,315,918 phnL b4097: phnK - carbon-phosphorus lyase complex subunit (NCBI), at 4,316,029 to 4,316,787 phnK b4098: phnJ - carbon-phosphorus lyase complex subunit (NCBI), at 4,316,784 to 4,317,629 phnJ b4099: phnI - carbon-phosphorus lyase complex subunit (NCBI), at 4,317,622 to 4,318,686 phnI Position (kb) 4316 4317Strain fitness (log2 ratio) -1 0 1 2 3at 4315.181 kb on - strandat 4315.192 kb on - strandat 4315.192 kb on - strandat 4315.200 kb on - strandat 4315.288 kb on - strandat 4315.408 kb on + strand, within phnLat 4315.507 kb on + strand, within phnLat 4315.516 kb on - strand, within phnLat 4315.516 kb on - strand, within phnLat 4315.585 kb on - strand, within phnLat 4315.585 kb on - strand, within phnLat 4315.720 kb on - strand, within phnLat 4315.740 kb on + strand, within phnLat 4315.740 kb on + strand, within phnLat 4315.751 kb on + strand, within phnLat 4316.095 kb on + strandat 4316.185 kb on + strand, within phnKat 4316.185 kb on + strand, within phnKat 4316.208 kb on - strand, within phnKat 4316.211 kb on + strand, within phnKat 4316.242 kb on + strand, within phnKat 4316.242 kb on + strand, within phnKat 4316.434 kb on + strand, within phnKat 4316.434 kb on + strand, within phnKat 4316.447 kb on - strand, within phnKat 4316.461 kb on - strand, within phnKat 4316.564 kb on + strand, within phnKat 4316.564 kb on + strand, within phnKat 4316.654 kb on - strand, within phnKat 4316.891 kb on - strand, within phnJat 4316.929 kb on + strand, within phnJat 4316.929 kb on + strand, within phnJat 4316.930 kb on - strand, within phnJat 4316.932 kb on + strand, within phnJat 4317.020 kb on + strand, within phnJat 4317.028 kb on - strand, within phnJat 4317.034 kb on - strand, within phnJat 4317.071 kb on + strand, within phnJat 4317.095 kb on + strand, within phnJat 4317.114 kb on + strand, within phnJat 4317.114 kb on + strand, within phnJat 4317.122 kb on - strandat 4317.154 kb on + strand, within phnJat 4317.159 kb on + strand, within phnJat 4317.168 kb on - strand, within phnJat 4317.177 kb on + strand, within phnJat 4317.177 kb on + strand, within phnJat 4317.210 kb on - strand, within phnJat 4317.215 kb on - strand, within phnJat 4317.303 kb on + strand, within phnJat 4317.340 kb on - strand, within phnJat 4317.349 kb on + strand, within phnJat 4317.394 kb on - strand, within phnJat 4317.402 kb on - strand, within phnJat 4317.428 kb on + strand, within phnJat 4317.428 kb on + strand, within phnJat 4317.481 kb on + strand, within phnJat 4317.481 kb on + strand, within phnJat 4317.620 kb on + strandat 4317.620 kb on + strandat 4317.631 kb on + strandat 4317.631 kb on + strandat 4317.634 kb on - strandat 4317.639 kb on - strandat 4317.658 kb on - strandat 4317.658 kb on - strandat 4317.686 kb on + strandat 4317.705 kb on + strandat 4317.708 kb on + strandat 4317.708 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic Sulfasalazine 250 uM
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4,315,181 - -0.1
4,315,192 - +0.2
4,315,192 - -0.3
4,315,200 - -0.4
4,315,288 - -0.1
4,315,408 + phnL b4096 0.25 -0.5
4,315,507 + phnL b4096 0.40 -1.1
4,315,516 - phnL b4096 0.41 +0.5
4,315,516 - phnL b4096 0.41 +0.1
4,315,585 - phnL b4096 0.51 +0.2
4,315,585 - phnL b4096 0.51 -0.4
4,315,720 - phnL b4096 0.71 -0.1
4,315,740 + phnL b4096 0.74 -0.6
4,315,740 + phnL b4096 0.74 +0.6
4,315,751 + phnL b4096 0.75 -0.2
4,316,095 + -0.8
4,316,185 + phnK b4097 0.21 +0.5
4,316,185 + phnK b4097 0.21 +0.8
4,316,208 - phnK b4097 0.24 -0.4
4,316,211 + phnK b4097 0.24 +0.2
4,316,242 + phnK b4097 0.28 +0.5
4,316,242 + phnK b4097 0.28 -0.1
4,316,434 + phnK b4097 0.53 -0.2
4,316,434 + phnK b4097 0.53 +0.0
4,316,447 - phnK b4097 0.55 +1.0
4,316,461 - phnK b4097 0.57 +0.2
4,316,564 + phnK b4097 0.70 +0.6
4,316,564 + phnK b4097 0.70 +0.1
4,316,654 - phnK b4097 0.82 -0.2
4,316,891 - phnJ b4098 0.13 +0.1
4,316,929 + phnJ b4098 0.17 -0.0
4,316,929 + phnJ b4098 0.17 -0.2
4,316,930 - phnJ b4098 0.17 +0.2
4,316,932 + phnJ b4098 0.17 -0.2
4,317,020 + phnJ b4098 0.28 -0.3
4,317,028 - phnJ b4098 0.29 +0.3
4,317,034 - phnJ b4098 0.30 -0.1
4,317,071 + phnJ b4098 0.34 +0.9
4,317,095 + phnJ b4098 0.37 -0.4
4,317,114 + phnJ b4098 0.39 +0.9
4,317,114 + phnJ b4098 0.39 -0.7
4,317,122 - +3.2
4,317,154 + phnJ b4098 0.44 +0.1
4,317,159 + phnJ b4098 0.44 -0.1
4,317,168 - phnJ b4098 0.45 +0.3
4,317,177 + phnJ b4098 0.46 +0.1
4,317,177 + phnJ b4098 0.46 -0.4
4,317,210 - phnJ b4098 0.50 -0.3
4,317,215 - phnJ b4098 0.51 -0.5
4,317,303 + phnJ b4098 0.61 -0.0
4,317,340 - phnJ b4098 0.66 -0.5
4,317,349 + phnJ b4098 0.67 +0.4
4,317,394 - phnJ b4098 0.72 +0.9
4,317,402 - phnJ b4098 0.73 -0.1
4,317,428 + phnJ b4098 0.76 -0.3
4,317,428 + phnJ b4098 0.76 -0.1
4,317,481 + phnJ b4098 0.82 +0.3
4,317,481 + phnJ b4098 0.82 -0.6
4,317,620 + +0.9
4,317,620 + -0.1
4,317,631 + -0.0
4,317,631 + -0.0
4,317,634 - +0.1
4,317,639 - +0.3
4,317,658 - -0.1
4,317,658 - -0.1
4,317,686 + -0.4
4,317,705 + +0.1
4,317,708 + -0.4
4,317,708 + -0.1

Or see this region's nucleotide sequence