Strain Fitness in Escherichia coli BW25113 around b3810

Experiment: LB Anaerobic Sulfasalazine 250 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyifL and dapF are separated by 36 nucleotidesdapF and yigA overlap by 4 nucleotidesyigA and xerC overlap by 4 nucleotidesxerC and yigB overlap by 1 nucleotides b4558: yifL - predicted lipoprotein (NCBI), at 3,992,545 to 3,992,748 yifL b3809: dapF - diaminopimelate epimerase (VIMSS), at 3,992,785 to 3,993,609 dapF b3810: yigA - hypothetical protein (NCBI), at 3,993,606 to 3,994,313 yigA b3811: xerC - site-specific tyrosine recombinase XerC (NCBI), at 3,994,310 to 3,995,206 xerC b3812: yigB - predicted hydrolase (NCBI), at 3,995,206 to 3,995,922 yigB Position (kb) 3993 3994 3995Strain fitness (log2 ratio) -1 0 1 2 3at 3992.653 kb on + strand, within yifLat 3992.693 kb on - strandat 3992.693 kb on - strand, within yifLat 3992.728 kb on + strandat 3992.728 kb on + strandat 3992.731 kb on + strandat 3992.738 kb on - strandat 3992.745 kb on + strandat 3992.761 kb on + strandat 3992.783 kb on - strandat 3992.790 kb on - strandat 3992.798 kb on - strandat 3993.601 kb on + strandat 3993.601 kb on + strandat 3993.637 kb on + strandat 3993.647 kb on - strandat 3993.778 kb on - strand, within yigAat 3993.778 kb on - strand, within yigAat 3993.782 kb on - strand, within yigAat 3993.813 kb on - strand, within yigAat 3993.813 kb on - strand, within yigAat 3993.815 kb on + strand, within yigAat 3993.827 kb on + strand, within yigAat 3993.827 kb on + strand, within yigAat 3993.829 kb on + strand, within yigAat 3993.837 kb on - strand, within yigAat 3993.837 kb on - strand, within yigAat 3993.854 kb on - strand, within yigAat 3993.892 kb on - strand, within yigAat 3993.892 kb on - strand, within yigAat 3993.948 kb on - strand, within yigAat 3993.948 kb on - strand, within yigAat 3993.972 kb on - strand, within yigAat 3993.978 kb on - strand, within yigAat 3994.051 kb on - strand, within yigAat 3994.088 kb on - strand, within yigAat 3994.090 kb on + strand, within yigAat 3994.090 kb on + strand, within yigAat 3994.093 kb on - strand, within yigAat 3994.098 kb on - strand, within yigAat 3994.098 kb on - strand, within yigAat 3994.098 kb on - strand, within yigAat 3994.098 kb on - strand, within yigAat 3994.098 kb on - strand, within yigAat 3994.098 kb on - strand, within yigAat 3994.170 kb on + strand, within yigAat 3994.192 kb on + strand, within yigAat 3994.192 kb on + strand, within yigAat 3994.202 kb on + strand, within yigAat 3994.210 kb on - strand, within yigAat 3994.279 kb on - strandat 3994.300 kb on - strandat 3994.300 kb on - strandat 3994.314 kb on - strandat 3994.318 kb on + strandat 3994.399 kb on + strandat 3994.765 kb on + strand, within xerCat 3994.776 kb on + strand, within xerCat 3994.932 kb on + strand, within xerCat 3995.087 kb on + strand, within xerCat 3995.093 kb on - strand, within xerCat 3995.093 kb on - strand, within xerCat 3995.095 kb on - strand, within xerCat 3995.113 kb on + strand, within xerCat 3995.165 kb on + strandat 3995.165 kb on + strandat 3995.246 kb on + strandat 3995.246 kb on + strandat 3995.251 kb on + strandat 3995.308 kb on + strand, within yigBat 3995.308 kb on + strand, within yigB

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic Sulfasalazine 250 uM
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3,992,653 + yifL b4558 0.53 -0.2
3,992,693 - +0.3
3,992,693 - yifL b4558 0.73 +0.0
3,992,728 + -0.2
3,992,728 + -0.9
3,992,731 + -0.1
3,992,738 - -0.3
3,992,745 + +0.0
3,992,761 + -0.5
3,992,783 - +0.1
3,992,790 - +0.7
3,992,798 - -1.2
3,993,601 + -0.4
3,993,601 + -0.7
3,993,637 + +0.5
3,993,647 - +0.4
3,993,778 - yigA b3810 0.24 +0.3
3,993,778 - yigA b3810 0.24 +0.3
3,993,782 - yigA b3810 0.25 +0.1
3,993,813 - yigA b3810 0.29 +0.7
3,993,813 - yigA b3810 0.29 +0.2
3,993,815 + yigA b3810 0.30 +0.6
3,993,827 + yigA b3810 0.31 -0.2
3,993,827 + yigA b3810 0.31 -0.6
3,993,829 + yigA b3810 0.31 +1.0
3,993,837 - yigA b3810 0.33 +1.3
3,993,837 - yigA b3810 0.33 +0.1
3,993,854 - yigA b3810 0.35 +0.7
3,993,892 - yigA b3810 0.40 +0.2
3,993,892 - yigA b3810 0.40 +1.4
3,993,948 - yigA b3810 0.48 +0.4
3,993,948 - yigA b3810 0.48 +0.8
3,993,972 - yigA b3810 0.52 +0.7
3,993,978 - yigA b3810 0.53 -0.0
3,994,051 - yigA b3810 0.63 +0.3
3,994,088 - yigA b3810 0.68 +0.5
3,994,090 + yigA b3810 0.68 -0.4
3,994,090 + yigA b3810 0.68 +0.2
3,994,093 - yigA b3810 0.69 -0.0
3,994,098 - yigA b3810 0.69 +0.8
3,994,098 - yigA b3810 0.69 -0.0
3,994,098 - yigA b3810 0.69 -0.2
3,994,098 - yigA b3810 0.69 +0.4
3,994,098 - yigA b3810 0.69 +0.2
3,994,098 - yigA b3810 0.69 +0.3
3,994,170 + yigA b3810 0.80 +0.6
3,994,192 + yigA b3810 0.83 +0.2
3,994,192 + yigA b3810 0.83 -1.6
3,994,202 + yigA b3810 0.84 +0.3
3,994,210 - yigA b3810 0.85 +0.3
3,994,279 - +0.7
3,994,300 - +1.3
3,994,300 - +0.0
3,994,314 - -0.2
3,994,318 + +0.1
3,994,399 + +2.2
3,994,765 + xerC b3811 0.51 -0.4
3,994,776 + xerC b3811 0.52 +3.2
3,994,932 + xerC b3811 0.69 -0.7
3,995,087 + xerC b3811 0.87 -0.4
3,995,093 - xerC b3811 0.87 +0.6
3,995,093 - xerC b3811 0.87 +0.0
3,995,095 - xerC b3811 0.88 +1.6
3,995,113 + xerC b3811 0.90 +1.6
3,995,165 + -0.4
3,995,165 + +0.6
3,995,246 + +0.3
3,995,246 + -0.1
3,995,251 + -0.4
3,995,308 + yigB b3812 0.14 -0.3
3,995,308 + yigB b3812 0.14 +0.5

Or see this region's nucleotide sequence