Experiment: LB Anaerobic Sulfasalazine 250 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfjV and yfjW are separated by 135 nucleotides yfjW and ypjI are separated by 523 nucleotides ypjI and yfjX are separated by 102 nucleotides
b2641: yfjV - orf, hypothetical protein (VIMSS), at 2,770,189 to 2,771,204
yfjV
b2642: yfjW - CP4-57 prophage; predicted inner membrane protein (NCBI), at 2,771,340 to 2,773,043
yfjW
b4644: ypjI - no description, at 2,773,567 to 2,773,838
ypjI
b2643: yfjX - CP4-57 prophage; predicted antirestriction protein (NCBI), at 2,773,941 to 2,774,399
yfjX
Position (kb)
2771
2772
2773
2774 Strain fitness (log2 ratio)
-1
0
1 at 2770.357 kb on + strand, within yfjV at 2770.357 kb on + strand, within yfjV at 2770.422 kb on + strand, within yfjV at 2770.433 kb on + strand, within yfjV at 2770.433 kb on + strand, within yfjV at 2770.570 kb on + strand, within yfjV at 2770.570 kb on + strand, within yfjV at 2770.594 kb on + strand, within yfjV at 2770.594 kb on + strand, within yfjV at 2770.602 kb on - strand, within yfjV at 2770.609 kb on + strand, within yfjV at 2770.609 kb on + strand, within yfjV at 2770.735 kb on + strand, within yfjV at 2770.743 kb on - strand, within yfjV at 2770.743 kb on - strand, within yfjV at 2770.796 kb on - strand, within yfjV at 2770.856 kb on + strand, within yfjV at 2770.951 kb on + strand, within yfjV at 2770.952 kb on - strand, within yfjV at 2770.952 kb on - strand at 2770.971 kb on + strand, within yfjV at 2770.971 kb on + strand, within yfjV at 2771.013 kb on - strand, within yfjV at 2771.065 kb on + strand, within yfjV at 2771.065 kb on + strand, within yfjV at 2771.085 kb on - strand, within yfjV at 2771.127 kb on + strand at 2771.127 kb on + strand at 2771.152 kb on + strand at 2771.167 kb on - strand at 2771.195 kb on + strand at 2771.195 kb on + strand at 2771.205 kb on + strand at 2771.208 kb on - strand at 2771.208 kb on - strand at 2771.208 kb on - strand at 2771.230 kb on + strand at 2771.244 kb on + strand at 2771.247 kb on - strand at 2771.279 kb on + strand at 2771.320 kb on + strand at 2771.332 kb on + strand at 2771.332 kb on + strand at 2771.371 kb on + strand at 2771.371 kb on + strand at 2772.159 kb on - strand, within yfjW at 2772.159 kb on - strand, within yfjW at 2772.290 kb on + strand, within yfjW at 2773.013 kb on + strand at 2773.098 kb on - strand at 2773.115 kb on - strand at 2773.123 kb on - strand at 2773.125 kb on - strand at 2773.132 kb on - strand at 2773.132 kb on - strand at 2773.137 kb on + strand at 2773.143 kb on - strand at 2773.145 kb on - strand at 2773.147 kb on - strand at 2773.147 kb on - strand at 2773.147 kb on - strand at 2773.147 kb on - strand at 2773.147 kb on - strand at 2773.156 kb on - strand at 2773.156 kb on - strand at 2773.203 kb on - strand at 2773.210 kb on - strand at 2773.215 kb on - strand at 2773.232 kb on - strand at 2773.248 kb on + strand at 2773.248 kb on + strand at 2773.256 kb on - strand at 2773.258 kb on - strand at 2773.281 kb on + strand at 2773.284 kb on - strand at 2773.284 kb on - strand at 2773.284 kb on - strand at 2773.286 kb on - strand at 2773.289 kb on - strand at 2773.289 kb on - strand at 2773.290 kb on + strand at 2773.326 kb on + strand at 2773.335 kb on - strand at 2773.388 kb on - strand at 2773.569 kb on - strand at 2773.634 kb on - strand, within ypjI at 2773.638 kb on + strand, within ypjI at 2773.638 kb on + strand, within ypjI at 2773.657 kb on - strand, within ypjI at 2773.675 kb on - strand, within ypjI at 2773.675 kb on - strand, within ypjI at 2773.805 kb on - strand, within ypjI at 2773.883 kb on + strand at 2773.883 kb on + strand at 2773.883 kb on + strand at 2773.883 kb on + strand at 2773.883 kb on + strand at 2773.897 kb on - strand at 2773.948 kb on + strand at 2773.956 kb on - strand at 2773.964 kb on - strand at 2773.980 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic Sulfasalazine 250 uM remove 2,770,357 + yfjV b2641 0.17 -0.1 2,770,357 + yfjV b2641 0.17 -0.5 2,770,422 + yfjV b2641 0.23 -0.2 2,770,433 + yfjV b2641 0.24 -0.9 2,770,433 + yfjV b2641 0.24 -0.1 2,770,570 + yfjV b2641 0.38 +0.3 2,770,570 + yfjV b2641 0.38 -0.1 2,770,594 + yfjV b2641 0.40 -0.1 2,770,594 + yfjV b2641 0.40 +0.1 2,770,602 - yfjV b2641 0.41 -0.1 2,770,609 + yfjV b2641 0.41 +0.8 2,770,609 + yfjV b2641 0.41 +0.6 2,770,735 + yfjV b2641 0.54 +0.1 2,770,743 - yfjV b2641 0.55 -0.1 2,770,743 - yfjV b2641 0.55 +0.0 2,770,796 - yfjV b2641 0.60 +0.3 2,770,856 + yfjV b2641 0.66 -0.9 2,770,951 + yfjV b2641 0.75 -0.4 2,770,952 - yfjV b2641 0.75 +0.4 2,770,952 - -0.4 2,770,971 + yfjV b2641 0.77 +0.0 2,770,971 + yfjV b2641 0.77 +0.3 2,771,013 - yfjV b2641 0.81 +1.4 2,771,065 + yfjV b2641 0.86 +0.1 2,771,065 + yfjV b2641 0.86 +0.1 2,771,085 - yfjV b2641 0.88 -0.9 2,771,127 + +0.2 2,771,127 + +1.1 2,771,152 + -0.4 2,771,167 - -0.0 2,771,195 + +0.4 2,771,195 + -0.3 2,771,205 + -0.5 2,771,208 - +0.3 2,771,208 - -0.2 2,771,208 - -0.8 2,771,230 + -0.3 2,771,244 + -0.3 2,771,247 - +0.5 2,771,279 + +0.0 2,771,320 + +0.4 2,771,332 + -0.2 2,771,332 + -0.9 2,771,371 + +1.6 2,771,371 + +0.3 2,772,159 - yfjW b2642 0.48 +0.8 2,772,159 - yfjW b2642 0.48 +0.2 2,772,290 + yfjW b2642 0.56 +1.6 2,773,013 + +1.6 2,773,098 - -0.3 2,773,115 - -0.3 2,773,123 - -0.3 2,773,125 - -1.7 2,773,132 - +0.9 2,773,132 - -0.2 2,773,137 + +1.6 2,773,143 - -0.2 2,773,145 - -0.1 2,773,147 - +0.5 2,773,147 - +0.5 2,773,147 - -0.5 2,773,147 - +0.8 2,773,147 - +0.5 2,773,156 - +0.0 2,773,156 - +0.5 2,773,203 - -0.1 2,773,210 - -0.3 2,773,215 - -1.6 2,773,232 - +0.0 2,773,248 + +1.6 2,773,248 + +1.6 2,773,256 - -0.4 2,773,258 - -0.5 2,773,281 + -1.0 2,773,284 - -0.2 2,773,284 - +0.6 2,773,284 - +0.2 2,773,286 - -0.3 2,773,289 - +0.2 2,773,289 - +0.3 2,773,290 + +0.3 2,773,326 + -0.2 2,773,335 - -0.3 2,773,388 - +0.3 2,773,569 - -0.6 2,773,634 - ypjI b4644 0.25 +0.2 2,773,638 + ypjI b4644 0.26 +0.7 2,773,638 + ypjI b4644 0.26 -0.2 2,773,657 - ypjI b4644 0.33 -0.3 2,773,675 - ypjI b4644 0.40 -0.5 2,773,675 - ypjI b4644 0.40 -0.3 2,773,805 - ypjI b4644 0.88 -0.2 2,773,883 + -0.6 2,773,883 + +0.1 2,773,883 + -1.1 2,773,883 + -0.6 2,773,883 + +0.2 2,773,897 - +0.6 2,773,948 + +0.2 2,773,956 - -0.5 2,773,964 - +0.1 2,773,980 - +0.6
Or see this region's nucleotide sequence