Strain Fitness in Escherichia coli BW25113 around b2217

Experiment: LB Anaerobic Sulfasalazine 250 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrcsD and rcsB are separated by 16 nucleotidesrcsB and rcsC are separated by 199 nucleotides b2216: rcsD - phosphotransfer intermediate protein in two-component regulatory system with RcsBC (NCBI), at 2,311,510 to 2,314,182 rcsD b2217: rcsB - DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI), at 2,314,199 to 2,314,849 rcsB b2218: rcsC - hybrid sensory kinase in two-component regulatory system with RcsB and YojN (RefSeq), at 2,315,049 to 2,317,898 rcsC Position (kb) 2314 2315Strain fitness (log2 ratio) -1 0 1 2at 2313.222 kb on - strand, within rcsDat 2313.297 kb on + strand, within rcsDat 2313.307 kb on + strand, within rcsDat 2313.338 kb on + strand, within rcsDat 2313.349 kb on + strand, within rcsDat 2313.349 kb on + strand, within rcsDat 2313.349 kb on - strand, within rcsDat 2313.422 kb on - strand, within rcsDat 2313.518 kb on + strand, within rcsDat 2313.521 kb on - strand, within rcsDat 2313.521 kb on - strand, within rcsDat 2313.525 kb on + strand, within rcsDat 2313.525 kb on + strand, within rcsDat 2313.590 kb on - strand, within rcsDat 2313.598 kb on + strand, within rcsDat 2313.671 kb on - strand, within rcsDat 2313.741 kb on - strand, within rcsDat 2313.750 kb on - strand, within rcsDat 2313.807 kb on + strand, within rcsDat 2313.959 kb on + strandat 2314.038 kb on + strandat 2314.038 kb on - strandat 2314.102 kb on + strandat 2314.202 kb on - strandat 2314.311 kb on + strand, within rcsBat 2314.319 kb on + strand, within rcsBat 2314.323 kb on + strand, within rcsBat 2314.362 kb on - strand, within rcsBat 2314.362 kb on - strand, within rcsBat 2314.439 kb on - strand, within rcsBat 2314.439 kb on - strand, within rcsBat 2314.490 kb on + strand, within rcsBat 2314.490 kb on + strand, within rcsBat 2314.565 kb on + strand, within rcsBat 2314.599 kb on + strand, within rcsBat 2314.773 kb on + strand, within rcsBat 2314.812 kb on - strandat 2315.032 kb on - strandat 2315.045 kb on - strandat 2315.139 kb on + strandat 2315.341 kb on + strand, within rcsCat 2315.548 kb on + strand, within rcsCat 2315.559 kb on - strand, within rcsCat 2315.678 kb on + strand, within rcsCat 2315.702 kb on + strand, within rcsCat 2315.745 kb on + strand, within rcsCat 2315.782 kb on + strand, within rcsCat 2315.785 kb on - strand, within rcsCat 2315.785 kb on - strand, within rcsC

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic Sulfasalazine 250 uM
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2,313,222 - rcsD b2216 0.64 -0.1
2,313,297 + rcsD b2216 0.67 +1.2
2,313,307 + rcsD b2216 0.67 -0.4
2,313,338 + rcsD b2216 0.68 -0.4
2,313,349 + rcsD b2216 0.69 +0.5
2,313,349 + rcsD b2216 0.69 +0.2
2,313,349 - rcsD b2216 0.69 +0.0
2,313,422 - rcsD b2216 0.72 -0.2
2,313,518 + rcsD b2216 0.75 +0.5
2,313,521 - rcsD b2216 0.75 -0.3
2,313,521 - rcsD b2216 0.75 +0.3
2,313,525 + rcsD b2216 0.75 +0.6
2,313,525 + rcsD b2216 0.75 +0.5
2,313,590 - rcsD b2216 0.78 +0.1
2,313,598 + rcsD b2216 0.78 +0.9
2,313,671 - rcsD b2216 0.81 -1.2
2,313,741 - rcsD b2216 0.83 +0.4
2,313,750 - rcsD b2216 0.84 -0.1
2,313,807 + rcsD b2216 0.86 +0.7
2,313,959 + +0.5
2,314,038 + +0.7
2,314,038 - +0.6
2,314,102 + -0.5
2,314,202 - +0.0
2,314,311 + rcsB b2217 0.17 +1.6
2,314,319 + rcsB b2217 0.18 +1.2
2,314,323 + rcsB b2217 0.19 +1.7
2,314,362 - rcsB b2217 0.25 +1.7
2,314,362 - rcsB b2217 0.25 +1.6
2,314,439 - rcsB b2217 0.37 +1.4
2,314,439 - rcsB b2217 0.37 +2.0
2,314,490 + rcsB b2217 0.45 +0.9
2,314,490 + rcsB b2217 0.45 +0.9
2,314,565 + rcsB b2217 0.56 +1.3
2,314,599 + rcsB b2217 0.61 +1.8
2,314,773 + rcsB b2217 0.88 +1.6
2,314,812 - +1.7
2,315,032 - -0.3
2,315,045 - +0.1
2,315,139 + +0.7
2,315,341 + rcsC b2218 0.10 -0.3
2,315,548 + rcsC b2218 0.18 -0.4
2,315,559 - rcsC b2218 0.18 -0.3
2,315,678 + rcsC b2218 0.22 -1.1
2,315,702 + rcsC b2218 0.23 -0.3
2,315,745 + rcsC b2218 0.24 +1.1
2,315,782 + rcsC b2218 0.26 -1.2
2,315,785 - rcsC b2218 0.26 -0.0
2,315,785 - rcsC b2218 0.26 +0.4

Or see this region's nucleotide sequence